CR00747

CRIMIC CR00747

Gene Trp1

CG4758

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:10010651..10010662	30F5	FBti0200655	83188

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAATGTCGGAAAAGAAGCGAGCCCG	CGTCTTACCCAGCTTTGGAATCCTTG

Images

Construct Design Sequences

sgRNA sequence	GGGGGACTACAAGCGAATATAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCCTCTCGCTCATTACCCA	AGCGAATATAGGCCACGACA	CCATTTAACCAATCGATCGTGAA	TCCTCGGCATCGCTTTCTTT

Amplicon 5'	Amplicon 3'
TCCCTCTCGCTCATTACCCACACATGTCAGGTGCAGGCCCACTTATCAGCTGCTCTGGTTGTTGTTATTGTGGTTATTCGTGTTGTTGTTGGGGAAAGTTTGAGTACGCGTGTAAGTTGGGCAGAGGCGGCGCTTAAGAGGGTTTTGTTAATCATTGGCTCCACACATACATGGGAAATTATCACTAATATCATTTCAATCGTTAAACAGCTATGAGTATCAATACAGCTTCAAACCCCCTTGCATATTTTCCAACTGCTCCCCTGTTTAGTATCCATGTGTTTGTTTGTTGTGGTGATCACCAGCTCGTTTGATTCAATACTTTCTAGACTCTCAGAGCGAATGCAACACGTCACGGCTAAGAAAAACTACAACCGCTTCTGGAATAGTTGTTTCTATTTATATAGTTCGCAGTTTTTAAGTTACGCATATTAATTCTCGTAATGAGTGGAGAGGAGGAGTACTTCCAGGAGGAGGAAGAAGAGGAGCAGGAGCAGGATATTTACGACGATGGCACGGTACTTAGGAAAAAATAAAACCCACGTATAATAAAGCCATCTCAACGGCTTGAGGGTGACGAATACAAAATTGGTGTCGTGGCCTATATTCGCT	CCATTTAACCAATCGATCGTGAAAGTGAGTTTCTGACCTGGTTTTCGGACCTTTTTCTCTTTTTTCCTCTTATAGGAATACACCGAACCGGGTGCCCAGAAGGTAGACAAGCCGTCAAAGGACGAGAAGAACGTGGCCAAGTGGCTGAAGAAGAACGTTAAGACGAAGAAGACCAAATTCCTGAGCCACATCGTCGAATACTTCACCTCCAGCAAGGCCATCGATGCTCTGCTAAAGTCCAAATTCACCGAAGGCAGCAATCCGCTTTTCACCACCCGGGAGCAGGTAATCGAGTTTCTAGACGTGATGCTGGAGCACAAGTTCTTCCATCGCGCCAAGAAGGTGCCCGTCACTCTGGAGGAGATCAGAGGAAAGTCTGGAGGCGACAAGAAGGCGGACAAGGAGAAGACCCAAGATAAGGAAAAGGACAAGGCAAAGGATGAGAAGAAGGATACCGATCCGGAAGGCGATAATGGCCAAGGAGACGGAGGTGCGTCTGGCAACGAAAAAGAGAAGGAGAAAAAGGAGAAGAAAAAGCGCAAGATCCGCCTGGACATGCATCCTGAGCAGATCTTCGTGGACGGCTCCGAGGCATACGTCTGGATCTACGATCCCATACCGCTCCACTACTGGATATTCGGCTTCATCCTGCTACTTGGCGCCGTTGGCATTTGCCTATTCCCTCTGTGGCCTCCACTGCTACGCAAGGGTGTCTACTACTTGTCCATTGCGGCTGCCGGCTTCCTTGTTTTCATCCTGGCTCTGACAATTGTACGTCTAATTGTGTTCATCATCGTGTGGGCATTAACCGGCGGCAAGTTGCACTTCTGGATCTTCCCCAACCTGACTGAGGATGTGAGCTTCTTCGCCTCCTTCTGGCCACTATATGAGGTTTGTTTCGAATTATCCATTTATGTTAGTTTACTAAATTACCTTCATCTCTAGAGCAACTATAACAGTGACCCAAATAATTCGTCCGCCAAGACCGACAAGAAATCGAAATCGAAGGACAAGAAGAAGGAAAAAGAAAGCGATGCCGAGGA

Amplicon 5'

Amplicon 3'

TCCCTCTCGCTCATTACCCACACATGTCAGGTGCAGGCCCACTTATCAGCTGCTCTGGTTGTTGTTATTGTGGTTATTCGTGTTGTTGTTGGGGAAAGTTTGAGTACGCGTGTAAGTTGGGCAGAGGCGGCGCTTAAGAGGGTTTTGTTAATCATTGGCTCCACACATACATGGGAAATTATCACTAATATCATTTCAATCGTTAAACAGCTATGAGTATCAATACAGCTTCAAACCCCCTTGCATATTTTCCAACTGCTCCCCTGTTTAGTATCCATGTGTTTGTTTGTTGTGGTGATCACCAGCTCGTTTGATTCAATACTTTCTAGACTCTCAGAGCGAATGCAACACGTCACGGCTAAGAAAAACTACAACCGCTTCTGGAATAGTTGTTTCTATTTATATAGTTCGCAGTTTTTAAGTTACGCATATTAATTCTCGTAATGAGTGGAGAGGAGGAGTACTTCCAGGAGGAGGAAGAAGAGGAGCAGGAGCAGGATATTTACGACGATGGCACGGTACTTAGGAAAAAATAAAACCCACGTATAATAAAGCCATCTCAACGGCTTGAGGGTGACGAATACAAAATTGGTGTCGTGGCCTATATTCGCT

CCATTTAACCAATCGATCGTGAAAGTGAGTTTCTGACCTGGTTTTCGGACCTTTTTCTCTTTTTTCCTCTTATAGGAATACACCGAACCGGGTGCCCAGAAGGTAGACAAGCCGTCAAAGGACGAGAAGAACGTGGCCAAGTGGCTGAAGAAGAACGTTAAGACGAAGAAGACCAAATTCCTGAGCCACATCGTCGAATACTTCACCTCCAGCAAGGCCATCGATGCTCTGCTAAAGTCCAAATTCACCGAAGGCAGCAATCCGCTTTTCACCACCCGGGAGCAGGTAATCGAGTTTCTAGACGTGATGCTGGAGCACAAGTTCTTCCATCGCGCCAAGAAGGTGCCCGTCACTCTGGAGGAGATCAGAGGAAAGTCTGGAGGCGACAAGAAGGCGGACAAGGAGAAGACCCAAGATAAGGAAAAGGACAAGGCAAAGGATGAGAAGAAGGATACCGATCCGGAAGGCGATAATGGCCAAGGAGACGGAGGTGCGTCTGGCAACGAAAAAGAGAAGGAGAAAAAGGAGAAGAAAAAGCGCAAGATCCGCCTGGACATGCATCCTGAGCAGATCTTCGTGGACGGCTCCGAGGCATACGTCTGGATCTACGATCCCATACCGCTCCACTACTGGATATTCGGCTTCATCCTGCTACTTGGCGCCGTTGGCATTTGCCTATTCCCTCTGTGGCCTCCACTGCTACGCAAGGGTGTCTACTACTTGTCCATTGCGGCTGCCGGCTTCCTTGTTTTCATCCTGGCTCTGACAATTGTACGTCTAATTGTGTTCATCATCGTGTGGGCATTAACCGGCGGCAAGTTGCACTTCTGGATCTTCCCCAACCTGACTGAGGATGTGAGCTTCTTCGCCTCCTTCTGGCCACTATATGAGGTTTGTTTCGAATTATCCATTTATGTTAGTTTACTAAATTACCTTCATCTCTAGAGCAACTATAACAGTGACCCAAATAATTCGTCCGCCAAGACCGACAAGAAATCGAAATCGAAGGACAAGAAGAAGGAAAAAGAAAGCGATGCCGAGGA

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