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CRIMIC CR00748

Gene Trx-2

CG31884

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:9615087..9615105	30C1	FBti0200656	83189

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAATAGAAGGAACTCACGTTCGGT	TGTGTCATTCCTAGATCCGACCTCAC

Images

Construct Design Sequences

sgRNA sequence	TATCGGGCGAGATAGTAGCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCACACATAAGTTGCGCG	CGATAAGCCTGTTCCCTGCA	GCCTCCCCAACGCAATATGT	ATCGGTTACAAACGGCGAGT

 

Amplicon 5'

Amplicon 3'

CTCCACACATAAGTTGCGCGCCATGCAAGTTGATCCAAAGCCAAGAAAACAAAAGACGCGAGTGCGCGGGGTGAAGAACCGGTTCCATTTCATGAATACAAAGTTACGTCAGGAAATATTGGGAAGTAAATACGTGAAAGCATGCCTTCTTATTTTATTTCTCAATATTAGTTTATTTATATTTGTATACACACTTAAACACTATCAAACATTGACTCTCGCGGTCTAGTTCTATACAGCAAACCACACAACAATAAGCTCAAAAATCAAAAAATGCTTATCGAAGGCATAAGGAATCCACGAAAACATCAAAACATTTATGCATACTGAATTAGACAATATAACAAAGTAGTTTAACTACTGATTTTAGTTTTTTATGTTTACTCAGATATTGTCTTAAAAAATGAGTCAGCAATACATGTTGACAAATTGAATTGTAGCCAACAGTCATCGAGTACCATCACCATCTTGAGATTAATCATTCCATGACGTATGTGTCTGCAATAGAAATGATCTAAGCCTGATTAAAATATGTAATAGGGATGATCAAGTGGTTTTGTGCTAGCAAAAATAGCGTGTATAAAGTTAAATTTCTTGGGCATATACTTATGCAAATTCCTCCAAACCGGTTGCCCATTCCAATTGAAATCGTCAATTTCATTAGAGCTCTGTGCTTGCATGTGCTGTTCTGCGATTGGCATCGCTAGCAATTAATTAAAGGTCATTGGGAAAACGTGTCACGGGCAGAGAAATGGGTGGAAGAAAGGGGATGGAACTGCCCACGACCGCCACACCTTTCGGCATACATACATATAATTTGGAAATTCTTTAAATATTGATTTGCTTTGCTGCCCAATGGGTGCAATACAGAAACTGAAATGAAATTGACCCGATTTCACAAGTTTATGAAGCACACACACATATTTAACCCGTCAATCGGCGATTATTGGGGCTGATTACAGCTTGGCAACTTTGGGGATGTCTGACAACGTTGTGTTTGCAGGGAACAGGCTTATCG

GCCTCCCCAACGCAATATGTATATTTACAATAGCACCGGATCAATGCTCCACAATCGGAAATCATAAAGTTTGAAAACATGACCACCACCTGCTAAAGGGAAAATTACTGAAACATCTCTAGGGAGTGGGAAATTATTAGCGCTAATGTATGATGATATTACTCAGAGATAGTACTAATTTACATTTTCATTTGCAGGCCGATCTCGATGGACAGCTGACCAAGGCATCCGGCAAGCTGGTGGTGCTGGATTTCTTCGCCACTTGGTGCGGACCCTGCAAGATGATCTCGCCCAAACTGGTTGAGCTTTCCACGCAGTTCGCCGACAACGTCGTCGTCCTGAAGGTAAATCTAAAAATGTGCTCAGCTTTATTTTATTTGCTTAACACTTTTTATGGAAATCCTTACTAAATGCCCTTCTCCTACAGGTCGATGTGGACGAATGCGAAGACATTGCAATGGAATACAACATCTCCAGCATGCCCACCTTCGTGTTCCTCAAGAACGGCGTCAAGGTCGAAGAGTTCGCCGGAGCCAACGCCAAGCGTCTGGAGGATGTCATCAAGGCCAATATCTAAGTGGGCAGCGCATAGACGTCTGGCCGTCCCCCTGATCCACCTATGAGTTTTCTTCATAACATTTAGTTAATTAATTAATTCCATAAGTGCAGGGTTTTGCTCCATTCCGCCTGCTCCGCCTTGTCCTTGAATCCCTTAGAGCTGCAGTGCAGTTCGCAAGGTGAAGGTCAGCAGTGGCAGCAGTTGGAACAGCAACAAACGTGAGAAACTAAGTGGCTTTTAAACGTAAAATTTGCTTTGTTAACAAAATCAATAAATCACACAAAGATTGTTCATTTTTGTATGAAAAACATTAGAAAACGCAGTTGTAGCCAGTTGTCCAAGAGAATACGCTAAACAATAAATGTATTACACACTCGCCGTTTGTAACCGAT

 

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