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CRIMIC CR00750

Gene IFT46

CG15161

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:18615615..18615615	36F7	FBti0200657	80640

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAATAAGTTCCGGAAAGACGGCGCCT	ATCTATTATTCCCACGCCTGACTGGC

Images

Construct Design Sequences

sgRNA sequence	TGAGTGGTAAAGAATTTCCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCTCGGCCAATCCTCCAG	GTGGTAAAGAATTTCCACGGTG	TACCACTCACCATTCGACGG	ACCCTGGCCCATAACAAAGG

 

Amplicon 5'

Amplicon 3'

GATCTCGGCCAATCCTCCAGGAGGGCATCTATGTCCTTTCTGGGCTGGACATCGTACATTGACTGAGTCATATGCACTTGTTCCACCTCAGTGATCCACTTCTCTATATCCTTGCCCGATCGGGCGGTGGGAACCAGTGATGCGGAGGAGGCGTGACGCGGATTTCGCCCCATGCTGCCACTCAGTACCGACCGAAGTTTCATATTCAGCAGACTGGGCTCACTTTGAGCGCCAGAGGGCTCATCGAGCAACCATAAGCCCAATTTCTCCAAGTGATCGTTCAGATCCTTCTGGCGTTGGGGCTGCAAAAGAGGCGGAGCCTGGACTTTCAGAAGGGCATCCACATCACCCACAGCGGGCACAAACTCGGGGATGAAAGGTTGCAGGTGAAACGGAGTATCGATCGTTTGCGGGGTGTATTTGACGATGTAGGGAAAGAGTTCCTTGAGTTCTCCGGGCACGGACAGATCCTCCCATTTGTCGGCACTCATAATAAAGGATTGATCATCCTGCCGCTTAGATCTTCCTGCTCCACTAGCACTTCCCCTCATGGGAAGCGTTAGCTTTTGATGTGGTAGCTTGGCTCCGCCTCTTGCTCCTCCGGATCCCATTCTGCCACCCATTGCGGCCTCCTCGCTGCCCGAGCTCAGGGTGTCATCCGGTACTTGCTTGGAACTTCGGTTGCTTCCCGGCCGCTTTTGCATGTTCGGCCCGGGCACCAGACCCGTCATTCCCACGCCCATGGTCATGCCCTGGGGCAGCTGCATCTCGCTGTCATCGTCATCCTCGCTGTCCTCCGCCTCGTCATCGTGATCAATGATGTCCTGCAGCAGACCATCGTCATCAGTAGAGTTGTTTTGGAACTGTTGCATGGAAGGAATATGCATATATGAAATTATTATATTCATTGAACAAGAATTGTACGCATTTAAATTGCTCCTTAATACTTATTTTCATTTCGTCACCGTGGAAATTCTTTACCAC

TACCACTCACCATTCGACGGTGCCTGCCTGCGGCTGCTCTTCGTACAGCTCCTTCTCCGCCCACTGCTCCTGGTCCACCACCGCCAGCACCACCTCTGCCACCGCCTGTTCCTCCATTTCGGGGCTCCATTTGAACACTGCTCAGGTGCTTGGAGTCGGGTCCGCCGATTGTGATCTCCTCGTCGTAGAAGTTCATGGCCAAGGACCAGCTGCGAGGACCCGGATTCAGATTCAGATTCAAATAGCTCTCCAGCTGCGAGTCCTCTCGAGCTATTCAGTTTCCTTTCACCGTTTTCCTGCCCACTTCCTGGCTTTCCGCGAAATTCGCACAACACTTGTCCCGCAATTTTCCGCTCCTTTTGCAGGTGCTGGGTCCTCGTCCTTGTTTCCTATCTCCAGAGCAGAGTTTTCTTCCTTTTCAGCGTTTTCCGCGTCGTTTTTTCTGTTTTCACTTGCTTACCTGCTGGAAACTTGTCAATTGCTTTGGCCAATGCCGGTGTCGATGTCGATGTGGTCGTCCTGACAACGCAACCCACTACGCTGCACTGCGAGAAATATTTTCGTGTGTTTAAACTGGAAGTACGTATATGTGCATTCATTTTTAAGAATTTGCATTATTCGAAACTGTTGTATATGACAGACAATACAATTATAATTTTTGTACACACGTTTTCTCTCTGTGCATCAAGTGGTTGTTATTGTCATATCCTTTTTGGCCTGCGTAAACATTTACAGCTGAAACAGTTGCCCTTTGTTATGGGCCAGGGT

 

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