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CRIMIC CR00751

Gene CG6115

CG6115

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:16887758..16887782	36B3	FBti0206842	83190

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CCTGCTGCGTTTGTTGATGTTGCTGT	CTACTTGCAATGAACGCGCGCTTTTC

Images

Construct Design Sequences

sgRNA sequence	AGTTGCGCACAGTTTCCCGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCATGTTTTCGTCAGCGCG	CCACCCCACCCAACGAAAA	GCGCAACTTACGTGCTTGTA	CTCGGACGAAAACACTCCCA

 

Amplicon 5'

Amplicon 3'

TTCATGTTTTCGTCAGCGCGAAACGAAAAGCTCAATCAGCTCGAATACGTCGGCGATTGTTTATCACCAAAACATGGACGGTTAAATGGTCAGCGTGTATATATTTATAGGTATACTCGTACTGGGGCGACCTGCGATCAGCGGTCAGATGAATTTAAATACGCAGTATTGTCAATACGCGACGGCGAGACGGCGCCCAACGCATGCGGGCATGTTGGCAGGCGCACCGAATATCTGATAGATACTTTTTATCACTGCGACATATCCGAAATAGACCCAGATAAACATACACCTGCCAGGCCCCTTCCGGCGGAATCCCTTGAAGGTCACCGCCCAGAGGGAAAGTTGGGGATCGAACAGAAAAAGAACTTACACCTGAAATGGGAATATGGCTTATTATTGCTAAACTATTTCCAACATTATCTTAGAGCACGAATGATAATGCCACACAAGTTACCATTGGAGGCCGCCAATGCCTTGGCTATTTTAATAGCCACATTTGTTTTGAAATCATAAAAGTCAATTGGTTTATTCAATGACTGCTTCGTTTATTCGCTACGTCCGTTTTTCGCTTTTTTTTGGGTGCCTCAATAACAAACTTTAAGTTGGACATTGACAAGACATTCGAAATTATTATTGTGTGTTTTGGGTTACGTTTCTTTTGGCCGAACAATATGCATTTCAGTATGTATATATTTTTTTAGTTTTTGTGTGCGACAAGCAAGGCGAAACGTATCATATTCTCATGATTCTTTCTACACCTCCTTCTTCGTGCTCCCCCCGATCCACAGCATCAGTCCTTGAGTTTTTGCAGCGATTTCCGCATCGCTGCCACGCCTCCCGCCCGCCGATTTACGTTCCGCCCACTTAGTCATTGTAACTATAGCGCTGCTTGACACTTCGGTACTTCTTCAGCGAGTACAGGGCCTCCACCTCCTTGGCCAAGTAACGTCCTTGGGCTAGCAGGGCGACGATCTTCTTGGGATCCTGCTCGTCCTTGTGGTTCATGAAGGCATCGTGGATCTGCTTCCTGAACTTCTGCGGCCCGTTCAGGCCGGGATATTCGCGGCCCAAATACTGCAACTGCAAGTGTTTACGCAATGTCAATTGGGGATGGTCTAGGTTGAACTTTTTTCGGTTCTGCTTTTTCGTTGGGTGGGGTGG

GCGCAACTTACGTGCTTGTAGAGGCTAATGACTTTCGAGCGCAGCTGTGACATCACCAGTGGTCAGCGGAATTGGTTTGTTTGTTAAATGCAAGTTAACAACTGATTTGCAGAAGCTTTTCTCTGCTTTTTGTTTATTTTATTTCAAGCGAAGTGAATCACACGGCTGTTTGAAAGGGACTAACGCGTAGCTGTGAGTTCCCCGCTAGCGATGGCAGCGTGAGTGCTTTTGGCGAAGGCACTAACTAATCTAGACTAGGTCGTGACTCATTCGTGACTCGTGAAGATACAAGTAGATAGAGATATTCATATGTGATACGAATATTATATTTTGAGCGTAATTTGTTGCACACTATTTAACAATGCATTTCAATAATAATGGTCAGTCGGATATTTCTTGTTATTGTTCTACCATTGAAATTAATGTTGCATTTCTTCTTTTGAAATAATAATGATAATTATAATGATAGACTATTTTTTACCTAAACGATTATGTTACTTTATTTTTTAAGATTATTTTTACAGCTAAAATAAATGACAAGTAAGTGTTGTGTCACAACACAATTAAATTTTATTATTATTCAAATGACAGCTGTACGCGCCATCTTCACCAGAAACCTGCGCATCTCTCCCGTGAGCCGTGACTATGGCGCGTCACCCGGCGTTGCCAGTCAGTTAATAGCTGCTTGTTGTGCATCCCCGATCGATCAGCTGTTCGCGAAGAAGAAGAAACGATGGGAGTGTTTTCGTCCGAG

 

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