CR00751

CRIMIC CR00751

Gene CG6115

CG6115

Information

Donor Location Cytology FBti Stock number
pM37-p02L:16887758..1688778236B3FBti020684283190

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK CCTGCTGCGTTTGTTGATGTTGCTGT CTACTTGCAATGAACGCGCGCTTTTC

Images

Construct Design Sequences

sgRNA sequence AGTTGCGCACAGTTTCCCGGCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTCATGTTTTCGTCAGCGCG CCACCCCACCCAACGAAAA GCGCAACTTACGTGCTTGTA CTCGGACGAAAACACTCCCA
 
Amplicon 5' Amplicon 3'
TTCATGTTTTCGTCAGCGCGAAACGAAAAGCTCAATCAGCTCGAATACGTCGGCGATTGTTTATCACCAAAACATGGACGGTTAAATGGTCAGCGTGTATATATTTATAGGTATACTCGTACTGGGGCGACCTGCGATCAGCGGTCAGATGAATTTAAATACGCAGTATTGTCAATACGCGACGGCGAGACGGCGCCCAACGCATGCGGGCATGTTGGCAGGCGCACCGAATATCTGATAGATACTTTTTATCACTGCGACATATCCGAAATAGACCCAGATAAACATACACCTGCCAGGCCCCTTCCGGCGGAATCCCTTGAAGGTCACCGCCCAGAGGGAAAGTTGGGGATCGAACAGAAAAAGAACTTACACCTGAAATGGGAATATGGCTTATTATTGCTAAACTATTTCCAACATTATCTTAGAGCACGAATGATAATGCCACACAAGTTACCATTGGAGGCCGCCAATGCCTTGGCTATTTTAATAGCCACATTTGTTTTGAAATCATAAAAGTCAATTGGTTTATTCAATGACTGCTTCGTTTATTCGCTACGTCCGTTTTTCGCTTTTTTTTGGGTGCCTCAATAACAAACTTTAAGTTGGACATTGACAAGACATTCGAAATTATTATTGTGTGTTTTGGGTTACGTTTCTTTTGGCCGAACAATATGCATTTCAGTATGTATATATTTTTTTAGTTTTTGTGTGCGACAAGCAAGGCGAAACGTATCATATTCTCATGATTCTTTCTACACCTCCTTCTTCGTGCTCCCCCCGATCCACAGCATCAGTCCTTGAGTTTTTGCAGCGATTTCCGCATCGCTGCCACGCCTCCCGCCCGCCGATTTACGTTCCGCCCACTTAGTCATTGTAACTATAGCGCTGCTTGACACTTCGGTACTTCTTCAGCGAGTACAGGGCCTCCACCTCCTTGGCCAAGTAACGTCCTTGGGCTAGCAGGGCGACGATCTTCTTGGGATCCTGCTCGTCCTTGTGGTTCATGAAGGCATCGTGGATCTGCTTCCTGAACTTCTGCGGCCCGTTCAGGCCGGGATATTCGCGGCCCAAATACTGCAACTGCAAGTGTTTACGCAATGTCAATTGGGGATGGTCTAGGTTGAACTTTTTTCGGTTCTGCTTTTTCGTTGGGTGGGGTGG GCGCAACTTACGTGCTTGTAGAGGCTAATGACTTTCGAGCGCAGCTGTGACATCACCAGTGGTCAGCGGAATTGGTTTGTTTGTTAAATGCAAGTTAACAACTGATTTGCAGAAGCTTTTCTCTGCTTTTTGTTTATTTTATTTCAAGCGAAGTGAATCACACGGCTGTTTGAAAGGGACTAACGCGTAGCTGTGAGTTCCCCGCTAGCGATGGCAGCGTGAGTGCTTTTGGCGAAGGCACTAACTAATCTAGACTAGGTCGTGACTCATTCGTGACTCGTGAAGATACAAGTAGATAGAGATATTCATATGTGATACGAATATTATATTTTGAGCGTAATTTGTTGCACACTATTTAACAATGCATTTCAATAATAATGGTCAGTCGGATATTTCTTGTTATTGTTCTACCATTGAAATTAATGTTGCATTTCTTCTTTTGAAATAATAATGATAATTATAATGATAGACTATTTTTTACCTAAACGATTATGTTACTTTATTTTTTAAGATTATTTTTACAGCTAAAATAAATGACAAGTAAGTGTTGTGTCACAACACAATTAAATTTTATTATTATTCAAATGACAGCTGTACGCGCCATCTTCACCAGAAACCTGCGCATCTCTCCCGTGAGCCGTGACTATGGCGCGTCACCCGGCGTTGCCAGTCAGTTAATAGCTGCTTGTTGTGCATCCCCGATCGATCAGCTGTTCGCGAAGAAGAAGAAACGATGGGAGTGTTTTCGTCCGAG
 

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