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CRIMIC CR00752

Gene CG14767

CG14767

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8723138..8723138	44E3	FBti0200658	80641

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AGAGATAGAGAGAGAGCGACAGAGAG	TGCAGCCTGTGTGCTGATAGATGACA

Images

Construct Design Sequences

sgRNA sequence	ACCCTGCTCAGTGTCACGCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGGTTGAAGGCCTTGCAAC	CCTTTGACCCTGCTCAGTGT	ACACTGAGCAGGGTCAAAGG	GTTTTGCTGCAGTTGGCTCT

 

Amplicon 5'

Amplicon 3'

CAGGTTGAAGGCCTTGCAACGACCCGAGATGCGGCAACTTGCCATCTTTAGTTAAATTATTCATCAGAAATTCATCATGAAATTTAATCTGCAGTGCGCCGCAGTATATCAACAATTGTCACTCTGGGGATGAGTCAACCAAACAACAAGCAACCTTCGATTTGCGGAATTGCTTTGTTTTTAAAGCCTCTAACGCCATACATATCTACAAAATGTAAACAAAGAAACCCTTGAGATTGGACAGATTAGAAAAGATAACTCCACTGCTGTCCACACCATAATCAACCCATGTGACGGAGTTTTCTACTGGAAAACGTGACCTGATCAAAGCATAAAGAAAGTAGCGGAACAGTGTAAAATATACACGAATAGCGTAGTGAGACAAATACTATTAAGCAAGGGTTTTTGCTCAACGACTCAAATCTTTTATTGGCCGCAGATTCGCGCTCTCTTAAGGGCAGAGCATTCTCTTTCATTTTCAGAAGAGACATCAAAGGTTTCTGGGTTTCCAAAAACATTGATAACAAGACTGAAAATACTGCACTTTGCACATTTATATCCCTTTTTTTAGCCAATAAAACTAATTTCTTATGAAACTTTTGAAAATTCGACAATGCTTTACATTTCTCCAAGTGTAGCGATTGCTAATGCGAAGTCAGATCTTGTGAAAGGTTAGTTTCGGACAATGGACATGGGATGTGGAAACTTCCGCAGTGGAACCGATGGCAGAAATAAGTTGTTGTGCCAAAAAGCCCCGATGATAGTACAGTATAATTCGCTTAGCTGCATCGATAGTTAGCTGCATCGGCAAGATATCTGCATTATTTTTCCATTTTTTTGTGTGAATAGAAAATAGAAAATAGAAAAAAAAAAATTAAGTTAGCTGCATTTTTAAGTTACCTGCATCGAGGCATTGTGCAAAGTACTCGAGGCAGCTAAGCGAATTATACTGTAGTTAAATTTGATCAGATGACGCAGCAGCGGCCAATCAGCCACCAATTGACGTCACAGGCGGGGAAAGTGCAGAGTTGGCTCGTCTTCGTCTTCGCAAAGTTTTGCAGTCATTGGCTGATTGATTTTTCCCTTGCGTGACACTGAGCAGGGTCAAAGG

ACACTGAGCAGGGTCAAAGGTTTTTCGCAAATGCCGCAGGACTGCATGCGTGGGTGTATCTCGATATACACACACGCATGTATGGATTAAAGGATACCCCCCTTGATGCACTTACCAGGTGCCACAATCCGATCATCAAAGTGGCCGTGTGGACATGCAGGCCGAAGCAGCAGGTGAACTCCTTCAGGTGACGGTTTCCGGTGCTGCTGCTACTTCCGGCGGCACCATTGTTGTTGTTGCTGTGGAGCTGGTTGCGTTGGGGACCCATCTTGAGGTGGATCCTAAACATTGCACGCCCCACGAAACTGCGGCTGTGGAAAATGAATGCGAAAACGAAAGCGATAACGATAACGAGTGCAGAAAATAATCAGCAATTGTGGGTGTTTTATTCCTCCCACGGATGTGTGTTTGTGTGTGTATGTGTGTTTAGGTGTAGGTGTGTGTTGCATCTACGAGTATGTATGTATGCATTTGTATTTATGTGTGCCACTTTTTCACGACTGATGCTTTGACCTTTTGCACTACTAATTCGGCAATTGATTCACTCTCCTTTATAAGGCGTACCTTTGATTTGTGGGCCAATGCAACTTGCACTGGTTTTCAACTCCGATTTATATCTGCAGCTCTTGATGTATTCAATTTTATTTAACTGCTGCGCAGCACGAAAACCCGTGAGCACTAATCTCTGAAAGTGGTAAAATGCCGCGCAACACGGTCACACTGACATTATGCGTTTTGCAGAACGGTAACGTTTATACGAGTCATTATTCGCAAATATCATGATATTATACAATAATTTTTGAATTAGATGTCATATAAATAAACAACAAAACGAAGAGATGTAAGCTCATTTATTTCTAAATATTCATAGTTTATGTATTTTGTGTCCCCAAATGTAAATTGATATGTTTTTATTACTATTATTGAAGTTAATATTATTTTTAAAATTTACTTCGAAAGGAGCAACTCAAGATAGGTTTGTGCTTCCGCATCGTAAAGTAAATATTAATTTTAAAAAGTACGGTAAGTGTGACCGGTGCGAACGCTAAAGCGCCAATCCGCAAACATCGATAGCAGCGATGCAATCAAAATACATGCTTTGCTATCGTAGGGTGACCATAACTAAAAGATTTGGAGAGCCAACTGCAGCAAAAC

 

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