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CRIMIC CR00754

Gene CG17059

CG17059

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:13220448..13220475	49F10	FBti0200659	80642

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTACGCGCCTTTTGCAACGATATCCG	GCTGAACAATGCTCCCAAGTACCAAG

Images

Construct Design Sequences

sgRNA sequence	TTCGATGGGAAGCTATGTTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGCGGCTAAGAGAATCGC	ATTTCCATGTAGCCGGGGC	TCCCATCGAAGAATATGGTCGTC	TGCAGAGGGCCTACAACTTG

 

Amplicon 5'

Amplicon 3'

AGAGCGGCTAAGAGAATCGCATGATGATCACAAATAAAGTTTTAAATATATCTAGAACACTTCTGTATTGTTTAAGAGCAGGAGGGGTGAATTAGAAAAGCATTTTGGAAGAATTACTTGACAATGGTGATTTATAGTTAACAAAACAATTCGAAATGCCTTTATATGCTTAAAGGATATTTGTTTTCGGGTGCCGTGTAGTGTACATTTATCAGTTTGAGGCGTTAGCCTAATATCAAGAAAATCCTAGGTAAAGTGCAGCAGGATTCACTTCTTCAGCTTAATGAACTTCGAGGCGAATTCATAAATGCCCACAGAGTTGTTGTTCTTCTGGTCGTACTCCACCCAGTCGTCCTCGGACAGGTCCACCTCCTCGAACTGCTGGCCATTTTCAGCGGATGAGACGCGCCAACCGACGCGCGGCGAGAATTCGACGGGCTCTGCGCCGCGGCACTCGAAGACCACGATGGTCTTAAATGCGCCAGAATCGTCCGCCGTGTAGGGAGCTGTGTGGATGGATGCACTCAGTAGTTTGGTGGGGATACGTTACTCAAAGCCAGCCTACCATTCGACTTGTCGACGATGTCGATGCTGTTCTCCCGGCTGCACATCTTGCACTTCATGAAGAAGTTAAAGCCGGCCGCATTGCGCGAATCCTGTTGCACACGTTCGGATTCCGTGATATCGTGCCACTTGTCCGACTGCTCGCCGCAGTTGCTGCACTTCAGCTTCAAGAAGAAGGAGTAGTCCGGATGAGACGTCTCCAGCTTGTCCACGTTCTCCAGCGTGGCGGAAATCTGAAGGCCCACACGAACCATTTTTACAATAGTTAATATTTATATTGAAGTTCTTAGCGGCAATCAGCTGTTTCATAGGGTTGCCGGACGGGTACCTATTTTCTGTGTGTCATTGCAGTGGCAACGCCTTCAGCAAGTAAAGATTTTTCTGCGCTAAAAAAAATAACAAAAAAGATAATTGGAAGCTAAAATAGAAATAAGTATGGATTTAAACATGAAACCGTCTCTGGCTGCCGACGAAATGTTTTCCGAAGGCCCCGGCTACATGGAAAT

TCCCATCGAAGAATATGGTCGTCGAAAACTTTGTTTATCCTTGTAGCCAACATTGCAATTAAAAGTAAACAACAATCAGATAAATCTACAATCTTCTTTAGAAGTAGTGTTTCCCCGCAAAACAGGAATATGTATCTTTCATTTCATCTTTTGGCGACTTTATTTATTATATTAATTGTTCCCATGTCCAGAGTTTTCATCAATGCTCATGGCAATATCCTACATAAATGTTAAAAACCTGCCACCATACGCTCATATACATATGTATATATTTATATTGTATTCCTTTAGTCCGGCGGAGCCACAGGAATGATGATGGACCACCTGCCCTCCAACGATAAGCACGTCCACGCTGACTTCTACAATGATTTTGATGATCTGTTCGATGAAGACAACTGGGCCAAGATGAAAACCGATGGGAAACAGTAGAGGCCACCACGCAATAAATGGATTGAAAGATTTGTATTCGCGACTCGTTCTTCTGTCTGTAATTTTACTTGCACAATCAGCGATCATTGGTGTATAAAAAATTGCTTCAACTATTGTTTAATCGCTTATTATATTCATTTGGTTCGTATAACTAAGGTAAATTGCCATGTGAGATAGTGCCGAGGTGGATATGAACTTTGGCTTAGCGTAAATCAACAAAATGCATAATTTTGTACAAAAAAACGAAACTATTAACTAAATTTATCGAACTGAGTTTCAGGTGGAATGAAGCAATCATAATTGAGATAAATACAAATGTACACAATAACCTAATGGATCCGTGGAAGTTGTTAACCAAGTTGTAGGCCCTCTGCA

 

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