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CRIMIC CR00756

Gene CG7102

CG7102

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:7885791..7885791	28D2	FBti0200660	80643

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTGGCAGAGATGAGCGCATATATGC	TCCCCTATGTAGATGATGCAGCGTTC

Images

Construct Design Sequences

sgRNA sequence	TTGCCTGTTCGGACGAATCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCGTGGGACTGACGTTCTG	GTTCGGACGAATCGAGGGAA	CGTCCGAACAGGCAAGCTAT	TTCCCGGATGCACAGTATCG

 

Amplicon 5'

Amplicon 3'

TTCGTGGGACTGACGTTCTGTATGAGCGTGATGTTGTCGCCCTTGAGCATGATCCTCCCGAGGTTGCGGCGCTGCCGGGTCTTCACATAGACTTCCTCGGCGTCGTCCAGCACCAGATTCATGTACTCATCGAATCCCACAATGTGGCCCTCGATGCGCAGCGATATGTTCTCGTATAACCAAACTTGCACGCGGGACCGGTTCTGCAGGTAACGGAAGATCAGGTTGATGGGCTGCACCATCACCTTCTGCACCTTGGGATTTCCTTTGAACGACATTTTTGCTGATTTCCGACGACTTAATTTGAGTTAAACAAATTTTCGCTTCTTTTTGCCGGCGTCTTCTTTTCGTCCAGGCGATAGGCAGAAATATCGATGGCACAGCACAGATGGTCACACATAATCGCGGCCTACCGAACTATCGACCAGCCTAAAGATACCAAAATGGTAAATCCTCTGGTAGCATAACCATATCGATAGAAACACTATCACAATCGCACTGTTAAGTTTTTGTGGTTGGGCAGAAGTTGTTGCCATTTATTCATAGCTGAATTAAACTATAGTTTATAAAGAAAACCAAACAAATGGCTATGGCTAATGTGGACAAAGGTGAACTTATGGACCAGGTTAAGCAACAGATTGCTGTTGCGAATGCCCAGGAATTGCTCACGCAAATGACCGAAAAGTGCTTCAAAAAATGTGTCAATAAGCCGGGAACTTCCCTCGATTCGTCCGAAC

CGTCCGAACAGGCAAGCTATACATATCTTATTTTGCACTATAGAATATGTATTTAAATAGTATTTCATTAATATCCAATTTAGAAATGCATTTCCATGTGCATGGACCGGTTTATGGACTCGTGGAACCTCATTTCTCGGGTGTACGGCCAAAGAATACAACGTGAACAATCCAAGTTCTAAACACACGAATCGGCTTCTGCATTTTGGAGCAGCTTGGGACTAACTATATCTGCCAGCTATTGATTAGTTGTTATATCTAAAGTTTGATAATGCAATAAAAACATGCATTGCGTGTTACGTGATTCTGATAACACAACGTGCATATGTTTTTTCTTATCGATATGTGATGTGGTACAAAAAATACCAAAACTTTCAGAATGGTTAGTCTCAATACATTTGACATAGCATATTATGTAGAATGTATAAAGTGTTTTGGCGCGAATATGCAACTTCACATAATTGCTTACTTTTAAAGGTGTGATTTTCTAAAAGAGCTACTCAATATTACTAACCTGCTTTTTCGTGTATATCCATAACGGCGGTTAAAAATGTGCAGGCATTATCCACCGACAATGTCGATATAACGTGATCCTCGCAGGCAGTTCGTAGCTCAACCACACCGATATCCTGTGCCAATATCATCATTTGAAATACTTGCGAGTCCTGGAGAACCAACTGAAAAATGAATTTTGGTTATAATTTTATTATGTTAGATGTTACTAATAAGTAGTTTTCTTACCTTTGCGGTGTAAACGTACAAGATGAACTGCCTGAATAGTTCCGGATTCACGTGTGGTAATCGTATGGTGGTCGGAGTTGAAGCTCCTGGGGCTGCTGGCGATACTGTGCATCCGGGAA

 

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