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CRIMIC CR00757

Gene TTLL3A

CG11323

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6738446..6738458	27A2	FBti0200661	80644

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGGCCAAGCAGAACAAGATCTTTAC	TATTCGGTGGCATTGAAGGTGGGCAT

Images

Construct Design Sequences

sgRNA sequence	GGCCTGTAGGTCTTTAGGCCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAATGGGTTTCGTTTGCCCG	AAGACCTACAGGCCCACATA	GCCAGAGGCTCTAGGATGTT	GCGAGCGAAACATCATGTCC

 

Amplicon 5'

Amplicon 3'

AAATGGGTTTCGTTTGCCCGACTTCAACTCACCCTTGACAACATCCTCCAAACACTGGGGACACATGCGCGCCGTCACACTCGTGGTGGCCCCTAGATCCGGACTGGAGTTGATCTCGATGAGCCAGGGATAGAAATTCTCACAGATCATGAAGTCGGCGCCGAAGAGCTCGAAGGTATTTGGCCGTCGGTCCATGTTCTCCTGTGAGGCTAGCATACAGCCAACGATGGCCTTGCGCATTCCGGGAAAGATCCGCTCCAGCCACATGTTGTACTTGCCAATTTGACGCAGGTACGCCTGGAAGGAGTAGCAATCCCACATGTTCTCGCTTGGCAGGCGCTTGTCCCGCTTTCCATTGGTGTACTTCTTTTGGATGGCGTAGTTGGTCAGGTGCACGGACTCGTGGTGGTTGCTCAGACTGTACTCCTGTGAACTGAATCGCAGGTAGCTCTCCCGGTAGAACCACACCACCAAAGGCTATGGATTTAGTAAAGGAGATTATGATGCGCTACTTGGAGTGGGTCGATATTCGCTCACCTGGGTGTTGGTTATCAGAAACCACTGTCGAATGTCGAATTTGGTTTGGAACAGAATCAATGGGCGTTCTGCAAGTACATAAATGGTTAGCTATTCCCTTGACATTAGTAACACTTTCTTAAATATTTCACACATAATGAAATTACTTATTATAAATAAATCCTTTTCATATTGATTGACTATCTTCATTCCGACCAATGACATTCCGGTCTGAATAATAAATCAATATCCTTGCGGCAAACCTGTTGCACTTCTCAGGCAGACAGAACAATAAAGAAAACGAGCACGACAGCGGGAAAAGCAATGATTGCGTTTTTAGGCCAAATATTTATGCATTTATGTGGGCCTGTAGGTCTT

GCCAGAGGCTCTAGGATGTTTGAGCCCATGTGCGTGCGTTGGGCGCCACAACTTACGATTCGAGTTCGTCGAAAACGAGGCCTACATAGGAAGCGAAAAAAAAACATCAGCTCGAACAACAAACTTTGGCACCGTCCTGATTTACGACCTACTTCCGTGTGTTTGTTTATGTTTTTATGTTTTAGGCCTGCACTTAAGATACTCTAGTCGCTATCGATTTGTAAAGGTGGAATTAGTTTCTAGTCAAAGAAATAAGTACTTAATGGCTTGTTTAAAAATTAAATCGTATCGATTGAATGAGATTTATGCCTTTCTCCAGCAATTTAAACTTCACTCACCTATATACTTTTGCACCACATAACGACTCTTGGAGGCAATCGAGAGATTAACCACGCCCAGTATTTTCTTGAGGTTGTCCATGAGGATGATGCCCCTGCCGCGGCACTTGTTGGCCGGCTTGACTATCCACATATTCTGGTATCCATCAAGGCTGTACTGCGGCCAGTGCACCTTCATCTTGTCCACCAGCGAGAGGCAGCTCTTGACCATCGGCTCCAGCCGCTGGCCACCGTCGTGCTGGATGCGACCATCCTCGTTCACCAGCTGCTGATGCTGGAACAGGAAGGCGTCCCAATCGTGCTCCCATATCTTGGGCGGATCCTCGAAGTCGATGTCGTTGTGCTGGCAGCTGTCGATATACTCCACCAGCCGCTTGTAGGCGAAGTCTATAGCGGAGTAGGGTATCTTGCCGCTGCAGGAGAAGACCGCATCGGAGCCCTGTTTGTGGTACTTGCAGAGCATGGCCCGCAGGAAGGCCACGCAGGCGGTCAGCTTGAAGTTCTCGATAAACTCGCCCAGCTCCTCGGGACTCCAGACGTTGTAGCACCTGGGAAAGTACATCTCGGCGGTACCCTCCTCGAAGAACCAGTGGAAGTCGCGCAGTTGGCTGCACAGACCCTCCTTCGAAGTGAACGGCGCTCGGTGGAATTTGTTGATGGTCATGCCTGGGTTCTTGGCCTGGTCGATGTAGTCGCACTTCTCCTTTCTGTTTGTCCACAGGAAATCGACGGGCATGTGCTCCAGGAAGCGGGACATGATGTTTCGCTCGC

 

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