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CRIMIC CR00760

Gene Mtor

CG8274

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:11853012..11853012	48C5	FBti0200662	80645

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTCGGATTCGTTGGTTGCCCCATA	TGGCGCTTTCCAAATTACTTTCCCGG

Images

Construct Design Sequences

sgRNA sequence	ACAAGAAGCGGTATCTGAGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGCTTGGTTTGGGTTTTGC	AAGAAGCGGTATCTGAGCCG	GCTTCTTGTAATAAGCAATCCGC	TTCGGAGATGCCCTGTAGGA

 

Amplicon 5'

Amplicon 3'

TCGCTTGGTTTGGGTTTTGCATTCGTTTATGTTATTAAATAAAGTCCTTTCTTTTTTTATTCGTAATAAAAACGGATTTTTCAACCACGTTTAAAAAATTTTTTTTATGAATGAATTTATCTCTTTTTAAAGAAAAACATTTTGTTTTATTAGGCTGTGAATAGGCTGTTATAACGCTGTTGAAACAGTGAGAATAATATACTCGAATCGATAGTACCGAAAAATATCGATATTGTTCCATCTCCACTGCATTGACGTTCATTTTTTCGCGTCCCTAAAGCGACTTTGTGCACAATTCGCAAAAAATTAAGACTAAAGTAAAGTAAAAAGTAAGAAAGTAAACTTCGCACCGCTCCATATAAATTTAACCGCTGCTTGACAAGGATCAACTGCGAACATGGATCTCAGTGGTCCACAAACTCTGAACAACATACTTCAGCCCGACGAGTTGAAACTCGTGCCGGAAGACGTCCAGAAGAAATTGTCGGAGTACATCAACAATTTCTCAGATGAGTACTGCAAGAACCGTGCGGCCGCCAATCGGTTGGGTAAGTTTTTATATTTGTATATATACTAAAATGCGCCATATTTTGCAAATAACCCTTTATTTGCATGCGTATGCTTTTACCTCCAACTTTGCGGCGTTTACCGGCTCAGATACCGCTTCTT

GCTTCTTGTAATAAGCAATCCGCGACTGTATGGTTTCTAGCGTGGTAAACACTCTTGCGCCATTTACATATTTTTTGTATAGAAATTTAAATATAAAATTCCGGGTTGTTGCAATTAAAAACAATGGCTGCTTGCAGCAACATTATTTTCCTTATTAATTTTCACCTCACCGGTGTGGTAACACTGGCCGGACATTTTCCCACCAAAACAGTTGATTAGTCCGAGTTTGAAATCTTCAGGGAATTTCTGTGTATTCATTTTAATCCAAATACTTTGTGGTTACTTAAATCCAGTTAAGAAGATTATTTATTTTTGCCCTTTTCACAGCCGAAGCCGAGCAAAAAAAGGAAGAGCTGGAGAACAAAATGGAGGACTATCTAGTAAAATTTACCAGCTTTGAGTTAAATGTAAACGAGCTGCGCACTCATCTAGATCAGATGTCTTCGGAACGAGTTAACCTAATGGACACGATCGCCAAAGGCGAGCAAACTATATCACAGTTGCGCAAGGAAAAGGCGTCGGTAGTTGAAGAACGCGATTCGATGATGAAGGTAATCGAGCGCCAACAAGCGGAGCTGGAACGATTGAAGCAGGATCTGCACACCTACCAGCAGCAGCTGAGCAGCGCGATTGCTGCCAAGTGCGAAGCCATTGCTCGCGTGGACGAAATTCAAAGTAAGGAAGTGGCTCTGGAGCTCAAAGAGAACCGCATGGAAAGCGAACGCGATATGCTGCACAAGGAGATTTTGTTAATCAGCGGAGATCTGAACAAAAGCAACGCCGAGTTACAGAACATCCGACGGGAGCACACAATAAACACCATGCAGCTACAGTCTTGCCTTAAAGAAAAGACCGAATCGCTAAAGCTGATGCAGGAACAGTACGAACAGGCCGTCAAGACCATCGGGGAACTAACCAGCAAGATTGAAATGCAAAACGACACCGCCTTCAAGCAGAACCAGGCCACGGAAGAGTATGTGGGGAAGCTGAAAAAGGAACTTGATGCCAAAGAGAAGCTTTTTGAAATCTTTAAGAGCACCGAATCCGATCATCTGATCCAACGGGAGGAACTCCTACAGGGCATCTCCGAA

 

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