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CRIMIC CR00761

Gene Arf51F

CG8156

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:15323600..15323603	51F6	FBti0200663	80646

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TGGCCAAAAAATCACGATATCCCCGC	TGGTTCGACGTTAACCAAATGAGGCC

Images

Construct Design Sequences

sgRNA sequence	AGGGTTCTCGAATTCGATTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTAACGGGCACATTGCTTCG	CGAATTCGAGAACCCTTTATTGT	TGTGGGCCTGATTAGTACCC	CGCGCATCTCCCTATCGTTA

 

Amplicon 5'

Amplicon 3'

CTAACGGGCACATTGCTTCGTGTATGTGTATATAAATACAAGAAGATTAGTGCAAAATTGTTGTTGTTAGTTATAGTTTTTTGGCCGCCGTGTGCTAGCAAATGCATTTTATGCCCCGGCCCGCGCACTTTGGCACTACATGTTAACGGATTTCAGGCTGGCGTGGAACGAATTGGGCTGGAAAGCGGTGCGTAACGGCGGTCGTCGGAGACTTGGGTGTGTATACATGTGTATGTGTGTTACGGGCGTGTTTGTGACGTAGGCAAGCAGCAGCGAGAGAGCGAGCGAAATTCCGTAGAATCCGAAAACCATCCGATATCGTTGCTCCCAAGCCGGCTTTACTGGGTCGTCGAGGGGTGCGGTGGCTGTTCAGGGGGCGGGGGTGGAGCGGTCGGATCCACCCGCCCATTCAGTGGAGCCAGCGATAAGCCCGCAATTGAGATAACTCTGCCTCAATTTGCGAGACACTATAGTGTGTTTATCTGTCCAATTGTACTTCCTATGTGTAAGCATGTGTGCGTGTGTTTGTGTGTGCGACTGTGTGCTGCAAGCTCTTCACTGATAAATTCTGCCTCTCCACTTTTTTCTCGCTTTTTAGCAACGAGCTGATGACAATGTAAGTGAAATTATTATGCAAAATAATGGTCTTAGCTTCCGCCGTCGTACAAGCTCTATATGGCCACCAAATTGCCTGTGCCCGCCGTATGTGTACATATGTAGCTACATTTGTATGTTCATATATCACTTACGCGCCTTCGCGCGACGGCGTTCTTATATCATAAATTGATATGTTAACTGCAAACTGTATTACAATGGCCCTATTATCTGGAAATTCACTGACCCTGCCCTGAATCTCGCCCAGCTATTCTTATCGATATGCTTATTTGGAAGACCTCGCCAACTCTACTCGAACTGTTTTTCAGATTGGCTAAGGTTTAATGGATGACACATCAGCACCATGGGAAAGTTACTATCAAAAATTTTCGGCAACAAGGAAATGCGAATTCTCATGCTCGGACTGGACGCGGCTGGAAAAACAAGTAAGCACATAAACAATAAAGGGTTCTCGAATTCG

TGTGGGCCTGATTAGTACCCATTAGTTTCATCAGTTTCCTAAGTGGAAACCAATCCCATTTAAAAAATACTCTTGAGGCGAGGCAATTATTGTTTTGTTTTTATTCTTATTGTACTTTGAAAAAATGTACAATATAAAGACCTTTCAATACGTACACCAGTACTATCACTAATCGAATTCGAGCTATTTTGTTTATACATCCTTTGCGTGTCTAAAGTAATATTGTTTTTGTCCACCCACAGCGATTCTGTACAAACTGAAACTTGGCCAATCTGTTACAACGATACCCACTGTGGGCTTTAATGTGGAAACCGTCACCTATAAGAATGTCAAGTTCAATGTGTGGGACGTCGGTGGGCAGGATAAGATTCGACCGCTATGGCGACACTATTACACGGGTACACAGGGTCTGATCTTCGTCGTGGATTGTGCGGATCGGGATCGGATAGACGAGGCGCGCACGGAGCTGCATAGGATAATTAACGATAGGGAGATGCGCG

 

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