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CRIMIC CR00762

Gene Wee1

CG4488

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6913336..6913342	27C4	FBti0200664	80647

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTCGAATGTGCAGAGGACGAAGCAT	TTGCTCTTGGAGTCAACAGCACTCAG

Images

Construct Design Sequences

sgRNA sequence	TAAGCTGGGATGGGTTAAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGGGCAGGTAACGACAGTC	ACCCATCCCAGCTTAGTCTT	TGGAGTGCAAATTAAAGCAACGA	CATCGGGTACGGCCACATTA

 

Amplicon 5'

Amplicon 3'

TTGGGCAGGTAACGACAGTCTCCCTCCTCGACGTAGGGTTCCTTAACGGAGGTCACGTGTCCCAGGTCGCCGATCTTGTACGTAACCAGATTCTCTGAATGGCGCAGTTCTTCATAGACGCTGTCCATGCCATCGTCATCTTTGGTCTGCTGCGGCTGCACCTCCACCAGCTTGTGTGCATTGGGATTCATGGTGGAGAATATGTTTTCGGGCTTCAGATCCATGTGCACCAGGTCATTTGAATGAATATAGCGTAGGCCCTCTATCACATGCATGAGAACAATCTTTAGCTCTGCCTCACCCAAACAATGATCTTGGATGCGCGCGTGCAAGCTGCCACCATCGCAGAATTCATTCTGTATCAGCATATGATCGTCCTCCGCCCACGCGGAATAGTATCTAACCACGTTATCGTGCTTGCCCAATACAGCATGGGCCCAGACCTCGTTCAGGGCTCTCTTTCTGCAAGCAAATAGATTGTTAAACGACGCTCGTTGCAATAACTAATGAATACTCACTCGAAAGAACTGCCTGCCACCGGCTTCTTACTCTTTTTAATCGCATATATGCAGCCATCTAGTCGATTGACGCATTGGAACACCACACCGAACTCGCCAACGCCGATTACGTTGACTTGCATGAACTCCCGCTTGAAGCGACTGATGTTCGTGTCGTGCAGGGCCAGCCGCTTGGGTGCCTGCTGGTGGATTTCGCGCATCGAGTCGCCCGCCTCCTCCGTCACATCGTCGTCGCAGGCATCACTGAGCAAATACTTTTGCATAGCATTCACGTTCAGGTTGAGGTTTTCGCGTCCGAATTGCGTGCGACAGCGCTTCTTATTGTGAGCCATGAGGCCTAAAAATAAAAAAAATGATTATTAGATTGCTAATAAGACTAAGCTGGGATGGGT

TGGAGTGCAAATTAAAGCAACGAACTGGAAATTGATACCTTTATTTAATTAAAATATCAAAAGCGAGAAACGTTTTAAACTTACTATCTGGCGTGAAGGGGTTCACATTTGCCGTGTCCTGGGTGGGCAGTTTGCGGTTGTGCAGTGGCAGAGATCGCGGGCGCTCTGACAGGCGGAAGAGGTCGTCGCTGCGCCTGGAGGCATTCACTGCCGCTGCTGCCGCCGACAGATGGTTGCTGCACTGCGTCGTCGACTTCTGCAGGATGGTCTTTGGAGTGGCCGGTGTGCTAAAGAGTCGCAGGGCCCGCACCTTTTGGCATGGCGGCGACATGGACACGTCCCCGTCGCCGCCTTCTTTGCCTATCTTACTGTCCAGCTGCTGCTCCTCACCGGAGGATAGCTTCGGCCGCTGCTGAAGGGGATGCTGCGGACTCGCTCCCTCGGGCGTGTCCTTGTCAAAGTCATCATCGGCGAAGCGGAGCTTGCGCGGATTGAAGACCTGCGGCGATGGCGAACGCGATCTCAGTTCGACGCTTGAATCGAGTGAGGTGACGCTCATCTCGTGCTCCGACTGGCGGAATGCCATTGTTGCTGTAGCTGTCCAGCTGACCACGTTGAAAGACGATTCTGTTCGTTATTTCTGATTTCAGAACTATTTGTATGGAAGCCGGCGGCAGTAAAGTAAAGGCAATAGTTAAAAAAAATGCAAAACAAAACACAAAACAAAATTTAACTGGAGGAATGCTAGTCTAATAATGTGGCCGTACCCGATG

 

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