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CRIMIC CR00763

Gene RabX1

CG3870

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23522268..23522271	59E2	FBti0200665	80648

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTGCCCAAGAAATCAGACGCAGCA	TGTATCCCAGATCTACGAGGAGAGAG

Images

Construct Design Sequences

sgRNA sequence	TGAAGCGAAATAGGGCTCACGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGCATTTTGGGGCAACGAC	ATAGGGCTCACGGGTACACA	CGCTTCAAGCATAATGCCGT	ATGGCTTCCGTAGTGCACAA

 

Amplicon 5'

Amplicon 3'

AAGCATTTTGGGGCAACGACATGTTCATTGGCGAAACTAAATCGAAGCACACAAAAGCTTACCAAAGAAACAAAGAGCCCCGCAAGCGAATCTAACAAGCGGAATACGAAAACTATATCCTATTATGTATTCATACTTATTGTGCGCCGGCGACTGCTTCTGCTGGTCAACTGGGGTCAACCGGGGGAAAAACAAATCTGACCTGTTTCTTTCAACATTCTGAGCAAGCACTTCGTCCAAGAAAAAGTATTGCTTTGCTGAAACGCTATGTGATTGGATTAAAGAGCTTGAATTCTATTCTACTAAAAAGTTTCAAACTTTCTCAGTTTAAAGCCTATCCACCTAGCTGCCTGTGCATCCATGTTAGAGAAAAAACAGTTTGGCGTTGAGAGTAAACGTATGGCCATTGTAGTGGCCATTGTTATATGGCTGCGCATAAAATCGGGATCACCGAAGAGTAAATACGGCAGCCAGGGCCAAGTGGGTGGTAACCCTTTGGCAATGGGCGAAGCTGGTGCTGCTGCTGCTCCTGCTACTACTGCCGCTCCCGTTTAGCTTATCAATAAACTATAAAAAAAAAGGAACGAGCTAACAAAAAAGAGAATAACACAAAGTGCAGCGGACGCACAAATTGCAAACAAAAAAGGACAAATAAAGTGACCAAAACAGTGGGCGGCGGAGACGGCGGACGAGAAGTACCCGCGGCGCAGCCAGTGGCAGAATAAACCCAGATTAGCGTTCCGGCAGCCCAGCCGGCAAATAGGATTAGTGGGCGCGTGGGGCAGTGGCAAATTCCTTTGGCTTTGCCTTTGGCTTCGGCTTTTTTACGAGGCAATTCCATTTGCGTTCGCCCCAAAAGAGTTGGTCGCACTGATACATATTCTGGCTGCTGTATACGCACTTGCAATTTAATTTTGACCTCGTCCAGGATGATGTTGCACGTGAAGAAGGACACGGCTATCGTGGGTACCTCGCTTTCCTTGCGGTGCAGCGTGTTCTTTATGTAGCGGATGACCAAACGTGTTTTGCCCACACCTGCAAGACAAGAACACAAATTATGCAATTAATCGAGAGATACAGCATTGTCTTTCTGTGTACCCGTGAGCCCTAT

CGCTTCAAGCATAATGCCGTGCAGCTTATCAGTGAACGTGGAATGGCGCACGCATTAATTGACCGCTGATTAATCACGCAAATTGAGCCGAATAAACAAAACAAAAACTGGAGACGCACTAGCCCGCAAAAGGTGGTAAACAGCGATAAGGAATCGCCTACCGACCACGAGATTCTTTGTTCGGCGCCCACTTTTTTGTGGCTCAGAGAAGCCCCTCTGTGTTTGATCAGACGGGGAATCTCCGCGTGGAGCTGCAGTATGTGCATGCACTGAATCATCCGGCGATTATGGGAAGAACGTATATTCCGAGGAAAGTACCGCCTTTTTTTTATCAATGCGTCCCTTTGTTTGGCGAAAAAGCAGAGCAAATACTGCACCCGCTGTGCATATAAGCCCCCTTAGTTGACCTTGGGCCAAACTTAAATACATATACTCATTGCGAATTCGTCAGCCGATTACGCTGCAAAAAATGTCCACTTTGTTGTGTAGAAGAGGGTAGACGGTGGAGGCTTCCCCCTTGATATGTTTTTGGAGTACAAATGTAGAAATGAACAATTTTAAGTTTCGGAGTTGTGCACTACGGAAGCCAT

 

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