CR00764

CRIMIC CR00764

Gene Psc

CG3886

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12975863..12975893	49E6	FBti0200666	80649

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GCCAATGTCCAGGGATTGGGAAAAGT	GCTCCTTTCGCAGGTGATTAATGAGG

Images

Construct Design Sequences

sgRNA sequence	TGGGGACGCCGGACGCTGGACGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGGACGAAGCACGAAGGA	ACTGGACAGAGTTGACAGTCG	TCCCCACTTCACATCGGTTT	ACAGACGACACGCAGATGTT

Amplicon 5'	Amplicon 3'
GAAGGACGAAGCACGAAGGACGAAGCACCAAGCAAGGGGCAGAGCATTAAACATGGAAATACAACCTTCAAGGCAAAGTGAGTGCCGTGCTGCAAAAAAGTTGTTTTGACTTTAATGTCAATGGCAGTGTCTGTCTCTTTCTCCGTGTCTGCGTAGCTCCATCCCCCTCCCCCTCCCCACCCCCTGCCATCGCGCTACGTCCACGGCCAGTTTTCCCCTGAATACATCCCGCCCGCTACAACTGTCACCCAGCAGCAGACCATGGCGATTCCCAGTGGGTGGGGTTCTGCATAAAGCAGGAGGCGAAGGATAGTATAGTCCACTGGGGGTCGCAGCACCGTCAGTCAGTCCAAGCTGGCCCGCCGCCTGTTACGCTTGTCCGTCAGTTTTGGTCCAATGCCGAAATAATAATAATGAAAAAAACACTCACACAGCCAGCGAAATACAAGGAAAAATTCGCACTGAGCTGAAGAAAAAAAATATATAAAGCAGGCGGTTTAGTGGGGCGTCAGTTGGGCGCTTTTGGGGCGGTGGCGTGTGCAAGCTAGCAAAAGGAAAAAAAAAAATGGTTTTACTGCCCGCCAAATGGCCTCGAATGGGGAGAGCAACAGCAGCCAGCTTTCTGCAAGAGTTGCAGCAGAATCGGTGCACTGAGAGAAATACGTAAGCCATACCAAATCAACTTTCTGACCCCTTGTCTCGTTAAAAACTTTTGTTATACAGTTGGTAATCAATCTTCCACTATCAAAATAGATACATTATTATATACCCAAACAAAATCTACAATTTTAGCTTGCTTTTTGTTTACAAATTTCTCGCAGTGCCCCCATGCAAAGGCAGCTGTTGCTACTACAGTCCGGTTTTCGGTTTGGTTTGGTTCGGTTTGGTTTGCTCTGCTTGCTCTGCGTTGCCTTTGCTTTTGGTTTCGGCGTTGGTTTGCTTTTGCTTTGGCCTGGGTCCCAGTGCCAGTGCCAGGTCCGGTCAGCAGTAAACAGTCCACCGACTGTCAACTCTGTCCAGT	TCCCCACTTCACATCGGTTTGTTTTGGTTCGGTTCGTTTGACAGTTAGATGGGACTCCCGGGTGCTCTACATACGTAAGCATGTACATACATACATATATCTCTGCGTCCCTGCCGGCCAACAGATTTAAATGAGCCTGACCAAATGTATCCAGTGTCCATTATCAACACATTGGTTGCCAGATTTACGGACGGCCAACGGAGTTTGGCAAGCAAAAAAAAAAAAAAGAAGAATCGGCTGAATGGAAAATTAACCTTACGCTGCAAAAGGGGCAACTTTTAATATCCGAACGGAATGGAGAACCGAACGGCTGTTGATGAATGGCCACATTCGCTAGTTTTATGGCGTTTAGTCCCCCGCCTGAATGCTAATGTTGTGTTAATATCTGTGTCGTTGATAATGCATCCGGGATTTTTACCAGTGGTGCGAGGAGCAGGGATCATCTCTCGCCCGCCACCTCGAACAGTTTCTCAATCAATCGAGCTAAGTACTTTCGAGGCCGGGACCATTAATGTGCCCACAAACTTATCGCCCGGCAACATCTGCGTGTCGTCTGT

Amplicon 5'

Amplicon 3'

GAAGGACGAAGCACGAAGGACGAAGCACCAAGCAAGGGGCAGAGCATTAAACATGGAAATACAACCTTCAAGGCAAAGTGAGTGCCGTGCTGCAAAAAAGTTGTTTTGACTTTAATGTCAATGGCAGTGTCTGTCTCTTTCTCCGTGTCTGCGTAGCTCCATCCCCCTCCCCCTCCCCACCCCCTGCCATCGCGCTACGTCCACGGCCAGTTTTCCCCTGAATACATCCCGCCCGCTACAACTGTCACCCAGCAGCAGACCATGGCGATTCCCAGTGGGTGGGGTTCTGCATAAAGCAGGAGGCGAAGGATAGTATAGTCCACTGGGGGTCGCAGCACCGTCAGTCAGTCCAAGCTGGCCCGCCGCCTGTTACGCTTGTCCGTCAGTTTTGGTCCAATGCCGAAATAATAATAATGAAAAAAACACTCACACAGCCAGCGAAATACAAGGAAAAATTCGCACTGAGCTGAAGAAAAAAAATATATAAAGCAGGCGGTTTAGTGGGGCGTCAGTTGGGCGCTTTTGGGGCGGTGGCGTGTGCAAGCTAGCAAAAGGAAAAAAAAAAATGGTTTTACTGCCCGCCAAATGGCCTCGAATGGGGAGAGCAACAGCAGCCAGCTTTCTGCAAGAGTTGCAGCAGAATCGGTGCACTGAGAGAAATACGTAAGCCATACCAAATCAACTTTCTGACCCCTTGTCTCGTTAAAAACTTTTGTTATACAGTTGGTAATCAATCTTCCACTATCAAAATAGATACATTATTATATACCCAAACAAAATCTACAATTTTAGCTTGCTTTTTGTTTACAAATTTCTCGCAGTGCCCCCATGCAAAGGCAGCTGTTGCTACTACAGTCCGGTTTTCGGTTTGGTTTGGTTCGGTTTGGTTTGCTCTGCTTGCTCTGCGTTGCCTTTGCTTTTGGTTTCGGCGTTGGTTTGCTTTTGCTTTGGCCTGGGTCCCAGTGCCAGTGCCAGGTCCGGTCAGCAGTAAACAGTCCACCGACTGTCAACTCTGTCCAGT

TCCCCACTTCACATCGGTTTGTTTTGGTTCGGTTCGTTTGACAGTTAGATGGGACTCCCGGGTGCTCTACATACGTAAGCATGTACATACATACATATATCTCTGCGTCCCTGCCGGCCAACAGATTTAAATGAGCCTGACCAAATGTATCCAGTGTCCATTATCAACACATTGGTTGCCAGATTTACGGACGGCCAACGGAGTTTGGCAAGCAAAAAAAAAAAAAAGAAGAATCGGCTGAATGGAAAATTAACCTTACGCTGCAAAAGGGGCAACTTTTAATATCCGAACGGAATGGAGAACCGAACGGCTGTTGATGAATGGCCACATTCGCTAGTTTTATGGCGTTTAGTCCCCCGCCTGAATGCTAATGTTGTGTTAATATCTGTGTCGTTGATAATGCATCCGGGATTTTTACCAGTGGTGCGAGGAGCAGGGATCATCTCTCGCCCGCCACCTCGAACAGTTTCTCAATCAATCGAGCTAAGTACTTTCGAGGCCGGGACCATTAATGTGCCCACAAACTTATCGCCCGGCAACATCTGCGTGTCGTCTGT

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