CR00767

CRIMIC CR00767

Gene net

CG11450

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:85936..85936	21B1	FBti0200668	80651

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	GTTCTTGAATGTCCTTCGCGGCTATG	GACCTCCACAAGTATTGAAGTGCCGA

Images

Construct Design Sequences

sgRNA sequence	AAGACGAGGCTTCCCACACAGGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGGACACACATTTACGGA	AAGACGAGGCTTCCCACAC	GTGGGAAGCCTCGTCTTATCAT	AGCAGACTTGTGAGGATGGC

Amplicon 5'	Amplicon 3'
TGCGGACACACATTTACGGAAGGTAAGCTTTTTGCATGTTTATTCAACTATTTATAAGCACAATATTCTGCTATCTTTTCTAATTTTAGGCAGATAAACAACGAAACGAAATTCAAAGCAACTGACTGACATTGCGGCCACGAAATCAGGCTGTACAAATAACATCAGTTTTATAGCCCTAACTCTATCCCATGTCTTGCGTGGTTTATATACCGCATTTTAATTTATATTTAGACAAATGTTTACTTTTAAGCCACCGTCCTCGCAGGCGTCGTCTAGTTTAAAACACCTGTGCCAAACGGAAACATTTTTAAAGCTTGCCAAGTTTTTAAAAAATAAATTTCTACCAATTTATATTTAAGTTGTCCGTATCAGACACTTAAAGTTACATCTAACGACCAAAGGGCAATACCTGCACATTTTATTTTATTTTTAGTATACACGGAAGAAGGTATTATTCTCGATTGTTTATATTAAGAATATTTTAAGATGTTTTTTGTACGCCCAAATTAGTTTATTTTTTATGGATCTGATCCGTCTTTAGGTGCCAAGTACAAAATAGCAAGTTTTGCTCAGAAATTGACAACGGTTTTCCCGTTATTGGCCTTTTTTTCTAAGAATGAATGCCCTCCTGCGAAAATCAGCTAAAACCTACTTAAAAACGGCAGACATTAACTGTCAGAGGCATAAGAAAATCCCATATTCACGCCAAAAGTCAACGAAAGGTTATCATCATAGGCGGCTTTTGGCTGGCGGTCGGTCCACTGGGAAGCGTTCCCTGTGTGGGAAGCCTCGTCTT	GTGGGAAGCCTCGTCTTATCATAACAAGTGCATATACTCATACGTACATAAATGTAAATATGTTATAGCGACTATATGAAATATATTGTAGTAAAATGACGCATCACTTTTATGAATGAATCGTAAGAACACATCCAGATCATGCAGTACTTTGACACACTTGGTAAGGACATTTTTACGTTGGTTATAAAACGAACCCAATATCATTCGGCTTTCCTGTTACTACATTGCGTTATGTAGTAAAATAATATAAGTGGAATTTTTATAAACTCATAGTGATCAATTTAAATTAAAGACACCTTATTCTATCCAAACCAATGGCCATCTAGACTAATCATTTCGAAACGTTGCTGATTAAGACTAGCATTTTAAATAAATGATCACAGAAACAAAAAATAAAATCGATTTTATGAAATGCTGACGTAAACAGTACCAACAATATGGAAGGAGTTAACGTCTGTCCTCGAACATTATAACAATCAGCAGGCGGTGGTGAAAAGGGACACACAATGCAGAGTCCAACTGTCGCTCCAGCTTCACAAACAGACAAATTGCCAATATGTCTGGCGACATCGTCGTCCCAAATTCGCCATTGCGGGCAAAGTGCTTGGCCGACATATGCAACACAAGACGAAAGTTGGAGTACGCTGGCGACACATAGTTGGCTTTATTTACGTATCTGTCTGCATCTTAATTGCCATCCTCACAAGTCTGCT

Amplicon 5'

Amplicon 3'

TGCGGACACACATTTACGGAAGGTAAGCTTTTTGCATGTTTATTCAACTATTTATAAGCACAATATTCTGCTATCTTTTCTAATTTTAGGCAGATAAACAACGAAACGAAATTCAAAGCAACTGACTGACATTGCGGCCACGAAATCAGGCTGTACAAATAACATCAGTTTTATAGCCCTAACTCTATCCCATGTCTTGCGTGGTTTATATACCGCATTTTAATTTATATTTAGACAAATGTTTACTTTTAAGCCACCGTCCTCGCAGGCGTCGTCTAGTTTAAAACACCTGTGCCAAACGGAAACATTTTTAAAGCTTGCCAAGTTTTTAAAAAATAAATTTCTACCAATTTATATTTAAGTTGTCCGTATCAGACACTTAAAGTTACATCTAACGACCAAAGGGCAATACCTGCACATTTTATTTTATTTTTAGTATACACGGAAGAAGGTATTATTCTCGATTGTTTATATTAAGAATATTTTAAGATGTTTTTTGTACGCCCAAATTAGTTTATTTTTTATGGATCTGATCCGTCTTTAGGTGCCAAGTACAAAATAGCAAGTTTTGCTCAGAAATTGACAACGGTTTTCCCGTTATTGGCCTTTTTTTCTAAGAATGAATGCCCTCCTGCGAAAATCAGCTAAAACCTACTTAAAAACGGCAGACATTAACTGTCAGAGGCATAAGAAAATCCCATATTCACGCCAAAAGTCAACGAAAGGTTATCATCATAGGCGGCTTTTGGCTGGCGGTCGGTCCACTGGGAAGCGTTCCCTGTGTGGGAAGCCTCGTCTT

GTGGGAAGCCTCGTCTTATCATAACAAGTGCATATACTCATACGTACATAAATGTAAATATGTTATAGCGACTATATGAAATATATTGTAGTAAAATGACGCATCACTTTTATGAATGAATCGTAAGAACACATCCAGATCATGCAGTACTTTGACACACTTGGTAAGGACATTTTTACGTTGGTTATAAAACGAACCCAATATCATTCGGCTTTCCTGTTACTACATTGCGTTATGTAGTAAAATAATATAAGTGGAATTTTTATAAACTCATAGTGATCAATTTAAATTAAAGACACCTTATTCTATCCAAACCAATGGCCATCTAGACTAATCATTTCGAAACGTTGCTGATTAAGACTAGCATTTTAAATAAATGATCACAGAAACAAAAAATAAAATCGATTTTATGAAATGCTGACGTAAACAGTACCAACAATATGGAAGGAGTTAACGTCTGTCCTCGAACATTATAACAATCAGCAGGCGGTGGTGAAAAGGGACACACAATGCAGAGTCCAACTGTCGCTCCAGCTTCACAAACAGACAAATTGCCAATATGTCTGGCGACATCGTCGTCCCAAATTCGCCATTGCGGGCAAAGTGCTTGGCCGACATATGCAACACAAGACGAAAGTTGGAGTACGCTGGCGACACATAGTTGGCTTTATTTACGTATCTGTCTGCATCTTAATTGCCATCCTCACAAGTCTGCT

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