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CRIMIC CR00768

Gene mle

CG11680

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:5977920..5977928	42A6	FBti0200669	80652

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAATACGCTAGTAACTCCCACATCG	AACATCTCCGGCGTAAGATTGTCCTC

Images

Construct Design Sequences

sgRNA sequence	TAACCTGCATTCAAATTTCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAAACCTTGCGTTGCTCGT	TGGGGTGAAACTCTTCGACA	TGAATGCAGGTTATACATACGGA	CGCAACAGGATGGCGAAAAA

 

Amplicon 5'

Amplicon 3'

GAAAACCTTGCGTTGCTCGTCTCGTGGAATCTGCGAGTGGCACGGCAAAATTTGGTATTGGGATGTATCACCAAAAATGTTTGTATTTTGCAGAAACTTCATAAGCGCAAAGATCAGGTTCCAACCGGGCAGGAATACTAAGATAGCACCGGGAATATTTTTTGACTTAATATGCATCAATAATGCCTCCAGCAGCTCAAAGCTAACGTCCGATTCGGATAGCATGGCCATAGCATTTCGAGTCTTTTGTGAATACTTGTCCTCGCACACCTTGTTATAGTTGATTTCCGCCTCGTCCTTGTCCTCGGAAAGCAATTGCTCCTCGTCCTCCACTTCCTTGCGCTTTCGGCGCGACTCCGCCGAAGGCACAAAGTCTGTCATCTGAATAATGTCCTCCAAGAAAAATTGCTGCACGGGAAATGCTCTTCCGGGTACTTCCAATACGGGACAGATGCCAAAATATTTGGAAAATTTCGTTGTATCAATAGTGGCCGACATAAGAATAACTGCAATAGAAGAGTTTCTATTAATTATAGTGTTTTCTAAAAGTCATATCATATTAAATTAGGACTAGAAATGTATGACCCGTAATGCATATAAGTTTTAAGTTCTGAATAGTAATTTTGTTGGGCATAAGTAATTTTGGCAAGTCAACTCTACATTAAATATGAAACACAAACAATTGGCAATGCAAATTTTCAATGGAACAAACTGACAAATCGACTATTAAGAACATAACATATACACTCTGGGCGGATCCGGTACAAACAATATAATCATAATCTTCATTAATTAAGCCAGTGAATTAGTGCAAAATAAGTTATTTTCGCGGCTCAAAATCTGCTCAACATTGCTTTTTATACATGTAAATGTATGTATATATACAATACATATATACAAATGTATGTATATATAAATATATAAGTATAATCAACAATTGTCGAAGAGTTTCACCCCA

TGAATGCAGGTTATACATACGGATTTATGTTAGAAATATTGCACCTTATTCGAGCTTACCATGCAGGTCCGGATATGTGTCGACCATGTCGCGAAGGATGACCAGCAAAAAGTCGCTGTTCACGTCTCTCTCGTGGATTTCGTCCACAATAATATGCGACACTCCTCGTAAGCCAGCCTCCAGCTTGCGCAGCAACACACCCACGGTGCAAAAGAGGATTGCGCCGTAGGGTCGTGGAAACACAGACTCAAATCGCACCGAGTAGCCTACAGTGTCGCCCAATTGCTCGCACCGCTCGCGAGCTACGCGCTCGGCCACAGAAATGGCTGAAATGCGACGAGGCTGGGTCACATAAATATTAGCGTACCCGCCCTGGCCGGAGCAGATGTAGTCATCAAGAATGTATTGGGCAATCTGGGTAGTCTTTCCGCACCCTGTGTTTCCACGAATGATTACCACTGGATTGTCATTAATTGCGGTTAAAATTTCCGACCGCATCGCTGCAATGGGCAGCTTTTCGCGGAACTCGAGGAATTGTCGGTACTCGTTGTCGTTTTGGCGGCGATCACGCAGCGAGCGTTCGTAGTCCATCGAAAGGTCATCAATAGAGGTGGTTGCCAGTTCGCCTTCGTCGATGTTGCAGGCATGCCACGTATTCCAGTTTGCCTGAGGAGGAGCCCACGGAATCACGGAGCTTTCCTGACGTTTTTCGCCATCCTGTTGCG

 

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