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CRIMIC CR00770

Gene H2.0

CG11607

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6016580..6016597	26B2	FBti0200671	83191

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGGTATTTGCCGTACTTCCTGCTG	TGGCATTTGGCTTGTGTCAACCATCG

Images

Construct Design Sequences

sgRNA sequence	TACCCGGTACAGTCATCCATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGGGTCTGGGTTGCAGATG	TGACTGTACCGGGTATGGGT	TCCCTGTCGTTGGCCTAAAC	GCCAAATTCCAGCACACGAT

 

Amplicon 5'

Amplicon 3'

AAGGGTCTGGGTTGCAGATGCATCTCCAGTAGGATGTGTTTGCGAATCGTACATTTCGCAGCCATATTTAAGTTGCATCGATTTATTAGCACGATGCATTCCACATTTACGACTTGGCGAATATTGTGGCTTATTTCGCGGTAAATTGCGAATGATCTTTTGGGGTTAAGCAGAGCACTCCCAGTGGGCCATGTGCTATGGAAATTTCGACTACCAGCTCGACAAGCGGAGTATGTGTTTATTTGATTGATTTTAATATATTATACATATGTATCTACTTCCGAACATCCGAATCTCTGACTTTTACCAGCTAACTATGCACTAAGATATATTATCTGCATTATGTACTTTCAAAATATTTAAGTGAAATCAAAAGTATAGCTAATATTTCAATTGTATATTTCGTGAAATATCTTTAGCTACTTGGGATTGTCGTCAATTTTCACTCAATTCATTATAGTTAATCCTATAGTTGTGGTATCTACATACATATATCAGTCCCGTTAATTTGAGTTGAGTGCGTGGTAATTATTATTGCTACAACCCTCCCCTGCTGTTGGGAACTTGAGAAAGAGTCTTCAAAGTTCCAAAAGAAATCGTCTGAGGGCCATCATTAATCAGGGATCTTCGAACGCTTTGAAAGTGCATCTATCAGTTGGATTAACATATAAATTGCTAATTAATTAGACCCACGGCGCCGGATTATTGTTCAGACAGAATTTCCATATCAGACAATGGTCTGTAGCATCCAATCGATCGATGGATGGATCGATTGATCGGCAGCCAGAAGAGCAACAACAACAGATCAACAAACTACTCATCGCCAGGCGGGACAGGCAAACAGCAACAAATAGTCGACGGAGGAGCCGCAGAGATCTCGCTGGTCCATGATTTTATGATGTGCGATGCGATGCAATCAATCCATCACGGAATAATTCCGCAGGGGCCGGCAGGTTGGACACGGCGGGCAGCCACAGGGTAGCCTCCTCCTGAAAAGGCCACCACAGTGGGTGGTTGTGTAAATACTTGGCCTTTTGGCTCGGCCCCCAAAGAACAGCGCATAGTTTCACCCGTGCCCGTGCCCGTACCCATACCCGGTACAGTCA

TCCCTGTCGTTGGCCTAAACGGCAGCCGAGCCTCATTGGAGTCGGTTGAGCCGCAGTAGTTGGAACCGTGGCGCCCAATCTATCAGAGATCCGCGTCTCTAGCCGGGCCAAAGATGCCTCCACCTGTCGAACTCCTCGGCCGGCGGAACAATTCATTAGAGAGCGGGCAATTAGATGGATAGCTATCTGTATCTGCCCATGAATGACGGGCCAATTGCCCAAGCAAATAAACAAACTGTTCGCCACAAACGTTTTCCACGATCCGTGTGAGCTTGAAGTTTATTTTTAAAGACTGACTCGTAATTTGTTCATAAATTGTTCTTAATTTTCCACTTTGGTCTTTGGCATCTCATCTATTTGCGGTGCCCAAAGGATTGATGAAGCCTTTCAGGTGCTGTCTGTTCTTCGTTTTTTTTTTACCGATCTTTTGTTGATTGAGCTATGTCGAATTTATTCGCCTTACTAATTGCTCCTCCCATTTTTGGCTGAGCAAATGTGTTTGAAAACCACTTAGCACTGATGATAATTTTCATTAATGTTCCATCATTGTGTGAAGTGTAATTTATTTAAGTTTGGCGCTAATTTTGCTACATAATTTAATCGTGTGCTGGAATTTGGC

 

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