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CRIMIC CR00780

Gene CG8677

CG8677

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:21221947..21221947	39B3	FBti0200675	80656

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGCCGTGTTCACCAAATGCCATAT	CTGCTGTCTACGTCTTCGATAGAACG

Images

Construct Design Sequences

sgRNA sequence	GCCAAGCACGCTCTCCAGAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCGTACAAGAGCTGTCTCG	CCAAGCACGCTCTCCAGA	CGTGCTTGGCTCGTCAGA	TGATCCTCTTGCTCGGGAGA

 

Amplicon 5'

Amplicon 3'

CCCGTACAAGAGCTGTCTCGCGGCAAGAAATATGCACCCTCGAAGTCTCGCAGTGCCGTGCGACCAGAGAACTTGATCCTTGGACAAATGACTCGTGTTTGCAGTGCCCGTTTTGAGGTTTTGCCAGAGTAGTATACAACACCCGAAAATTTTCGCTGGTTCTCGGGGACGCATGAAAAATGGCGGCGTCCTCAGCTGCGGCTGCGGCGCCCACCAATCCGATCCCGCTTCCGACGGACTCCATATCGGAGGAGTGCAAGGACGACGACGCCCTCACCTCCACGCTGCACACATGCGCCAATGATCCGGACTTTGCGGTCATTTGCGCCTTCCTGCAAAAATTCGGCAAGGACCTGGGTCTTAACCTGCCCAACTTCAAGCATCTGCAGGAGTGGTTGACAAACAACAACGATGGTGAGAGGGTGGGGTGGTGGTATCAAGAGAGCGGGGTGGAAGCAGTTTTTTGCCAAATTAATTTATGTCAGGTTCAATTATCGTGGGGCAAGTGGCGGTGGAAACTACGGATGTAGTGTTTTCGAATAGGTCTGCTCCAGAGATTTAACAATGCTCCGCTCTGGAGAGCGTGCTTGG

CGTGCTTGGCTCGTCAGAGAGAAAGAGATTGCTCAATGGCAGAGAGAGCACTGCGATTTGTTTTCATCTCAACACAAGCGCAATATACAAAACAAATCTGGGAGAGACGTAAATATTTCCCACATTTGCTCTGGTCTTCTTATTTGAGAGATAGTAAGAGAGCTACAATTGAACACTAAAAGTTGGTTTTTTTTTTTTAATGTACACCTGCATCTGGAAATATATTAAATATTTAGGGTCACGAGTGGGAGGGAACCTGAAAATATATTTGTTTAAAAAAGTTATAATCATGCTAAGTTTCTACGTTTTGTATGTATTTGCGTGAGCAACAGCAGACACAAAAAGTGTACACTACAACAAACGCGGTTGCATTTATTTCACTTGCCCTCATAATCCACCCACAATCCATTTATATTCCGCTGTCACTTTCTTCCTTTTCGCAGAACTTTTACTAATATTTAATTATAACGAATCTTTGTAGTTCCTGAGCTAAGGGACTTGCACATAAAGCTGCTCCGTAAGACCCGTAAGACGGTTCACGAAAAGAGTTGGGAGTCCGCCCTCAGCAAATTCTGCTTCGGCTACTCGGTGCAAGATGCCTGGGAAATCGAACGTTTCGGTTATAGGAATTCCAGCTTGAAGGTTAAGCTGCGAATATTTCGGGTGAGTAACGAATTTCGTACCACTTGATAAACCGTTCTAAAGGGCGTCTTTCCCGTACAGGAACTTCTCGAAAGTCAGTTTGAACGCAATGCCAAGTTTCGGGCCCACATCCTCACCTTGAATGCCGATACTTTACGCTCTGAGCCAATTGGAAGGGACCGGTTGGGACATGCCTACTGGCTGACTCAGGATGCCCACTGTAATCTGCGGATCTACCAGGAGCATCTAGACGAGGAAATTTGGCAGGTGGTTGCCACGAACAGGGACGAGTTTGTAAATCTCATTTCAAGGCTGCGCGGAAATGAGGTGGTACTTCCTTCGAAGGACATTGGTGAGGCCGATGAGGACACCAGCAGCAGCAACAGTTGTCATCCCAAACCACCGCCCCCCGAACAGAAAGAAGAAGAGGACGATGAGGAAGTTGGAGATGAGCCCGAAAAAACTGTACCCAATCTAAAGATTAAACTCCGCTCTCCCGAGCAAGAGGATCA

 

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