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CRIMIC CR00781

Gene frj

CG9526

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:6457116..6457136	26D5	FBti0200676	80657

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTGTCTGGTATCCGTTGGCTCCTA	CCAAGTAGCTGAAGGCGAACCACTTA

Images

Construct Design Sequences

sgRNA sequence	ATAGGAAACTAGGTTGAATCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGCATCGACGACGTCATCT	CACCTGTAAGAACTCCGATGT	CCTAGTTTCCTATCCACAAGCAC	GAGTCCGGTGTAGATGCGAG

 

Amplicon 5'

Amplicon 3'

GAGCATCGACGACGTCATCTATGTGATCTGCCTGCTGGGCTGCATCGGAGCCGGCAGCTATGTGAAGAAGATCGCGGACGAGGGCCAGCGCAAGCTGGTGTCCACTGGGCTAGGCGTTCTTGTGGTTGTCATTGTCTCGGGGCTCCACAGTCTGCACTGCTTTGTTTCCCTGGCACTGGGCACGGCCGCCGTGCTTCTCGTGCACCCGAGGTTAGTTGGTGATCCTGAACCTGTGGAAAATTACCAATAACCACACTGCTCCCTCTTCCAGCAAGGGTCACCTGGTCACGTTTGCGGTCATGTTTGGCTACCTGGTGTTCTTCCGCATTTTCGACTTCTACTTCGGCATTCCCGGCCACACGAACATGATACAAATGATACTAACACTAAAGGTGGGTTGCATAACCATTCATTGCCATTACTTAGCTCGTAATCCTTGCATCGGCAGGTTTCGGGCATTGCCTTCGAGAAGACTGCCGCCTGGAAGCGACTGCAAGCGCACGACGAGCAAAAGAAGAACGACCAGCGGGATGTGCACCAGGAGAGTCCAATCGAGATCACCGACTACGATGTGGAATTGCAGAGCCTGAGTGCCGCTGAGATCCTGCACTACAGCTTCAACTACATCGGAGTTCTTACAGGTG

CCTAGTTTCCTATCCACAAGCACTAAAAATGTAGTCACAATACAACCAGATACCAAAGTCCAATTCAATTGCTGATTGTAAAACATTACACACACATGCTTATAATCGATTTCAACCGCATCAATATAATCATCAGTATCAAGTAGATAATAATTTGTAAATCTGCATTTAAAACGAAACCACTCATGTCCATTGATTGCAGGTCCCTATTATCGGTATCGCACATACAGGGACTACTTTGAGATGCCTTTCAAGACCTACGCTCCAACTGTTGAAGCCACCCTGGAGAAGCTGAAGTACGCCGTCTTCTACTGCGCATTATACCTGGCAACCAACTACATGTGGCCACTGGATGTGAGTGCTTGAGCACACCGCAAAACATTACTAAATTGAATACATTTCAGTATGCGTTGAGTGATGAGTTCTTCAACGATCGCTCCTTTGTGTACCGTTTGCTGTACGTGTGGCCCACGTTCTTCACATTCCGCGCTCGCATCTACACCGGACTC

 

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