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CRIMIC CR00782

Gene Flo1

CG8200

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:15568943..15568962	52A13	FBti0200677	80658

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATAGCTTTTCCGGCCATCACCTCGT	ACAGAGGATCCATGTGGACATGCAAG

Images

Construct Design Sequences

sgRNA sequence	AATGTTGTTGTTGGGATAACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTCATCGCGCAAATCCTTG	AGCTGAATATGCGGCAGTCC	ACAACAACATTTGCGGTACAC	ATGCGAGACTTTGTCCAGCA

 

Amplicon 5'

Amplicon 3'

CCTCATCGCGCAAATCCTTGATGGTGTAGGAAACCACGGTTATTCCCATGTTGGCCAAATCGCTGGAGGCCACCTCAAACACCTGCTTGCTGAACTTCTTGCGGTCCTTGTAGATCTCCTCCACGGTCATCGAACCCATGATGGCACGCTGATGCCCCTCCAGGGTGACCAAGGCGATGTGGTTGATCTCTGCCTCTGATTTGCCCAAGAACTGCTCACAGGCGGTCAGCAGCATGTCCTCGTTCTGACCCTGGACCTTCACCTGTGCAATGCCTGTCACCGAGATGGGCACTCCTTGGCTGGTGTACACGCAAGGACTCTCTACCTGGAGGGTCATCGTGTTCAACGAAATTCTGGGGAAGATTGGCGGACATTTAACATCTGAGGGCTTGTTATCTTAAGACTAGGGACTCAGATACGAAGCCAGAAATATTAACTAAATATTATCTGATAAAATAAGATTATTTTATTATAATTTAATATATTTTAATAAAACCGAATTCTTACGAATAATATTTCGAATACCCGCGCTTTTTAGCGCCTCCCTAACCTGTGGCTTTTCGTTGGAACCCCTGGTCTAAGCCGGTTAAAACTCACCGCTGAACCTGCTGGCCCACTGGCCAAACGAATGCTCGTCCTCCCGGCACCAAAAGCGGCTTCATGTAGCAGCACCCTGCGCAGAAAACAAACAAAAAACACTTTGTTTACAAAACATTGCAGCATTTGGATAACAGCACCGCAACGCCGTTCAAATGCGCACGCATGGCTTCGGACTGCCGCATATTCAGCT

ACAACAACATTTGCGGTACACAAAAATCGATAGTGACGTCACTTGGGGTGGGGTGTATATATATGTGTGTGTGCGTGACAATTTCATTTTTATTCACTGACTTATTTATTTAATCGATTTGACATTATTTTGAGCATATAAGGATCGTGTGATTTACCAGAGACAACTAAAGCCTCGTTGGGGCCGCAGGTTACAAATCCCCAAGTCATTTCTGCAGCACTTATTTGTTATTTATATTAGAAAATGGGTTGCTGCTGATAACAGCTTTTGTTTTATCTGCGCGGTTAACACAGTTTTCCGATTATTAACTATATAGCGAATTATCCGATCCACAGGTAGCCTTGTTTATTCGTCTAATAATATATATAAAAGAATTTCCGAGGTACTCGGTGCTTAAGATTCAGAAATCCGACCGGTTCGGTTAGCAATTCGTAGCACAATAAAAAAATCTCTCAATCTGTAAAGTTCTCAATTTCTAACTCGCGAGTGCGCAGAACGACTTTAACCCCTATTGAGTGCCGGGGGTTGAGGCGACCACTTCAAAATATTCATCATGCTGCGACGACGTAGTGCTGCGGTAAGTGTCTTTATTGGTTTAAAAATCTAGGTTACATCTCAATTTTTTTTATTCTAATTTACTTAATGACTCATATCATTCAGAAAGATCGAAATTCTTAAAAATACACAATAAATGTAATAAGAAAATCGTCGACACTCATTAAATTTGGAAACCAGATGTATTGCCTAGTTTTTAGTCTTTGAAAACAGAAACTAGGATTTCAAATATTTTTTCCACATTCACACAAACAGTAGATATCGTCTAGGAATTTGAACAATTTTCGGATCTTAACGATACACTCAACATAAAAATTTTTAAGAATAAAACTATTTTACTGAATCAATAAGTATTTTATCGATAAATGTTAATTTGGCAGCTCAAATGGCTATATTAAGTGTCACATTGGTAGGGATTGTCATAAATCAAACCTTTTGCTTTGGTAAATCAAACTAATGAAATTATAAAGAACTTTTTATCTTATACTATAATCGAACTCTACTGCAAGGACATACATATATAATTAAAAGATCTTTGCTGGACAAAGTCTCGCAT

 

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