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CRIMIC CR00784

Gene Sin3A

CG8815

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12581160..12581185	49B6	FBti0200678	80659

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGCGAGGACTCCACTTTTGTTACA	CGCATGTAGCCGCAGGAATAAATACC

Images

Construct Design Sequences

sgRNA sequence	AATTGACAGTGAACTGATGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTTGGCTTGCCTTCGGTTC	CACTGTCAATTAACCTCCAACAT	TGACCACGAGAACTTACCGTC	TGTCACGAATGTCCACCGAG

 

Amplicon 5'

Amplicon 3'

CTTTGGCTTGCCTTCGGTTCCTGAAACAGTTGATGACGATGCCGCTGATGCTGCCGCCGCTGCTGAGGATGTTGATGGCGTAGCATCGTCCACAGGCGTCGCTGAAGATGCCGTTGCCGAGGACGCTGGTGCAAAAGATCCTGTCGTCGCTTTCGAGTCATCAGTCTCTTTCTTAATTCCCGCTGGTGTTACGCTGCTAGTGCTTGCATTGCTACGCGCCGGAGAGGCACTTTCACTGGGCGTACTCTTGGCATTTCGAGTCGAAGACTCCGTGCGACCCAGTGGCGAGTCCACGTCCATTTTCGTATTGTCATCTACCAAAAATGGAAAGGCTATATAGAGAAACGGTGTGCACAACACATATGTTCGGATATTCGATGCAGTTCAGGGGTCAGTGTGTGGGTGTTGGTGCATTGCAGTCCAGTTTTGGTTTTATTTTATTTAATAGCATTTTGATTACGTTACAAAATTTTGTTTTTATTTGTTTATGTTGTTTTTGTTATTAATTGCAAAAAAAAAATGGAGAAAAAGAAACAAAAAACATACACTATGTTAAAAAATACAATCGGGCAAAAGTTATTAGGGAGAAACTTTATGTATTGAAAGAAAAATAGTCATGCAAATTTCTATCGAGAAGTAAATTTTTTTACATGACTAGTTAACGTTTCAAAATCACAAAGCACTACTCCTTAATAAAAAAAAATGAATATAAATATTGTTAGTATGAGTACTGATGATGATGAGTTTTAAGTCAACTCTTAAAGAGATCTTGAAAATATTAGTTAGTTTTAGAAGCTACTAAATTAAAGAATAGTGTTAGCGCAACCGAAAAATTAAATATGTTGGAGGTTAATTGACAGTG

TGACCACGAGAACTTACCGTCATCGCGTTCGTCATCGCTTAGTGGCTGTCGCGGCGCGAAGAAAAGGTCAGGCACAAATTGTCGGATGATCTGCTTAATCTTTTGCTTCTCCTGCTTCTGGATGCCAGTCTGACGCTTCACATGATGAATCAGCAGGTTGGCGGCATCGTCCAGAATGGTTTTATCCTTGTACGGCAGCACCAGATGCGGCCCAAACGGTTCCATGGCGTCGTCCTCTTGGTCGTGCCGCTCATCGTACAGCGTCTCGATTTCGTTAAACAGACTCTTTGAGCGCAGGGCTTTCATGTCGTTGGGCTTAAAGTTGATGGCCTGGTGATCGAGAGACTTGAGGTAGTACTTCTCGTTCTGTTCCCGCCATTGCTTATTGAAGGTCTGAAAAAAGAAGGGTTCATTTAATTTACATAAATGTAAAGCAATGAGAGAGTTCACTTACCTTTTGCGCTTCTCGCCACTCCTCCTCCTTGACTTTCAGTCGCTTCAGTACAATAGGCACCGCCACAAACGGATTTTTCTTCATTCCCGTGATTATCTCTCCCGACTTGTCGCCGTAGATGCGATGAATGGCCCGCTGGTGTATCGTTTGAGATGTGCCGCCCAGATGATCGTCCAGATGGAATTTGCTCAATTCCTCGGTGGACATTCGTGACA

 

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