CR00788

CRIMIC CR00788

Gene CG6495

CG6495

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:10763536..10763557	32A5	FBti0200681	80660

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAATCGCAAAGTGGTGAGTTGTGGT	CGTGGAACAACACAACGAATCTTGGC

Images

Construct Design Sequences

sgRNA sequence	CACTGGAGCGCCGTAGCCTCCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAATCGTGAAGATGCCGCTG	TCCGGGTATCACTCCCCATT	ACATTAGCACACAAATGGCCG	TGCTCCAGGGCAAGTTCAAT

Amplicon 5'	Amplicon 3'
CAATCGTGAAGATGCCGCTGCCTGGGCCAACCGCAAGATGATTGCCGAAGGGCAGCAGCAGCTGCCGCAGCAGCAACTGCAACAGCAACAGCAGCAACAATCATCGCAGCCAAAGACGGAGATCATCAACACGGGTTAGTATAGATAAGGGGTCTAGGAAAATATAGTTAAGATTAGAAGTTAGTTGATAGGATGTATTAAAAGCATATGATGTGTCAAAGATTAGATTTAATGATTATTAACACAACAAAGTTTAGGAAATGAAACTTTTGCAGCTGTAGTATACGATGTAGTTCCCAATTGATGATATCTGTCTTATCTATAGAATCTTCAACGCTAACAAACTTATGATTGGCAATCATACAGGGCTTTTCTTCATTTTTTTTATATAATTTATTTTATTTTATGCCTCACAACCCAGTAAAGCTGCTGCAGCACTGCACTAAGTTGCATTAAGTTGTTAATTAAACTCGATTGAGTGTCCAGGAGCAATTAGTTCTAGCCAGGCACTCCAGCCCAACAAAAACTAAAAGCTGAGTAAATATTTATGAGGACAGGGAATGGGGAGTGATACCCGGA	ACATTAGCACACAAATGGCCGGCACAGTGCACGCAATGATCCCACTGCCATGGCCACTTGGCCACGACACACTCGCTCCCTTGTTCCGGAGGGCTTTTCTGCTGGGAAAATCGCAAATAGCCCATCAGTTTTCATTGTGTGAAATGGGAGCTGCCGTCGCTCGAAATTGATTTACGAATGCTTGTGCCCTATGCGTGCAGTGCCTCTGTCTGCGCGTAATTAATTCAATTAGCTGTGCGTTAACCGGGATACACACACTTCATGCTTCCTAGTGACGCCTTCGCCTCCGCTTTCTTACAGACGCACAGAGCCACATATTTAGCCATAAAGGCGGCCTCCAGCTGCAGCAGCCCGCAAACGGACAGGGTCCACCGCCGTCGCCACTCCAGGTTCCGGGTCAGATGGCGGGAGCAGGTCAGGTTCAGGTTCAGGGGCAATCCCCAACGCTGCAACTGCCCGGCTACGAGGCCAACCAGGCTGTATACGGGATGTCACGCACAGGAATCTACATGCCACAGCAGCCGGCGCAACTCCAGGCTCCAGCTGGCCAACAGCAGCAGCAGGTGCAAGCGAGTGTGGTGTGGCCACAGCAGGCTCCATTAATTCCGCAGCAACAGTCGCCCGTGCAGCAGTCGCAGTCCCTGATGCCCCAGCAATCTGGTCATGTCTATGCCGCCGCTGCTCCGGCCATCCAATCTCCGCCAGCTGCGCAGCAAGCCATGTTGCCAGTCCTAAATGCAGCACCCGTAGCTGGGAAAAATCAGGGTCCAGCGGCAGATGGGGACTACGATGATTACGATGAGGAAACGTACACGGAGGCACCAAAAAAGGTAAGATCTGATGAATGAATATAGATTAAGATTAACCATTCATTTTAAAACCACAGAAGCAGCACAAACACAAGAAGGTCAAGACGAAGACGGCCAAGGATCCCATTGAACTTGCCCTGGAGCA

Amplicon 5'

Amplicon 3'

CAATCGTGAAGATGCCGCTGCCTGGGCCAACCGCAAGATGATTGCCGAAGGGCAGCAGCAGCTGCCGCAGCAGCAACTGCAACAGCAACAGCAGCAACAATCATCGCAGCCAAAGACGGAGATCATCAACACGGGTTAGTATAGATAAGGGGTCTAGGAAAATATAGTTAAGATTAGAAGTTAGTTGATAGGATGTATTAAAAGCATATGATGTGTCAAAGATTAGATTTAATGATTATTAACACAACAAAGTTTAGGAAATGAAACTTTTGCAGCTGTAGTATACGATGTAGTTCCCAATTGATGATATCTGTCTTATCTATAGAATCTTCAACGCTAACAAACTTATGATTGGCAATCATACAGGGCTTTTCTTCATTTTTTTTATATAATTTATTTTATTTTATGCCTCACAACCCAGTAAAGCTGCTGCAGCACTGCACTAAGTTGCATTAAGTTGTTAATTAAACTCGATTGAGTGTCCAGGAGCAATTAGTTCTAGCCAGGCACTCCAGCCCAACAAAAACTAAAAGCTGAGTAAATATTTATGAGGACAGGGAATGGGGAGTGATACCCGGA

ACATTAGCACACAAATGGCCGGCACAGTGCACGCAATGATCCCACTGCCATGGCCACTTGGCCACGACACACTCGCTCCCTTGTTCCGGAGGGCTTTTCTGCTGGGAAAATCGCAAATAGCCCATCAGTTTTCATTGTGTGAAATGGGAGCTGCCGTCGCTCGAAATTGATTTACGAATGCTTGTGCCCTATGCGTGCAGTGCCTCTGTCTGCGCGTAATTAATTCAATTAGCTGTGCGTTAACCGGGATACACACACTTCATGCTTCCTAGTGACGCCTTCGCCTCCGCTTTCTTACAGACGCACAGAGCCACATATTTAGCCATAAAGGCGGCCTCCAGCTGCAGCAGCCCGCAAACGGACAGGGTCCACCGCCGTCGCCACTCCAGGTTCCGGGTCAGATGGCGGGAGCAGGTCAGGTTCAGGTTCAGGGGCAATCCCCAACGCTGCAACTGCCCGGCTACGAGGCCAACCAGGCTGTATACGGGATGTCACGCACAGGAATCTACATGCCACAGCAGCCGGCGCAACTCCAGGCTCCAGCTGGCCAACAGCAGCAGCAGGTGCAAGCGAGTGTGGTGTGGCCACAGCAGGCTCCATTAATTCCGCAGCAACAGTCGCCCGTGCAGCAGTCGCAGTCCCTGATGCCCCAGCAATCTGGTCATGTCTATGCCGCCGCTGCTCCGGCCATCCAATCTCCGCCAGCTGCGCAGCAAGCCATGTTGCCAGTCCTAAATGCAGCACCCGTAGCTGGGAAAAATCAGGGTCCAGCGGCAGATGGGGACTACGATGATTACGATGAGGAAACGTACACGGAGGCACCAAAAAAGGTAAGATCTGATGAATGAATATAGATTAAGATTAACCATTCATTTTAAAACCACAGAAGCAGCACAAACACAAGAAGGTCAAGACGAAGACGGCCAAGGATCCCATTGAACTTGCCCTGGAGCA

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