• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00789

Gene CG34194

CG34194

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:17544752..17544752	54C3	FBti0200682	83194

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAACTACAGGGTAAGCCTTGCTATC	GTGAAGTCGCCTCCTTGACACATCTT

Images

Construct Design Sequences

sgRNA sequence	GACTAAGACCGCCGAGTGACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCTCTACGGCTTCACCCTA	CCGAGTGACCGGCATAGATA	GGCGGTCTTAGTCATGGAAC	CACTTTCTCTGCCTCCGGTT

 

Amplicon 5'

Amplicon 3'

AGCTCTACGGCTTCACCCTAAACTTTGCGTACACCAATCTGGCGGCGATATCGGAGCGCATCCGGACCAGCTGCATCCACTACAACCACGACGCTTCGGTGGAGTTCTGTGTGGCTGTCCATTTGCACGCCCACGCCAACGACGTGCTGTCCGTGTGGCTGTTTCTGCTCTCCATGGTGCCATTGGTGGAGTGAGTCACAATCAAGCCCAAAAGTCGGCACACTCAGAAATGCCAGAGGCAATCCCCCTGCCATTAGGGATTCGCTATCTCGGTTCAGTTTACAAGTGCAAGCGTAGCCGCATGGACTGATTTTTATCGCAACGCCCAACTGACGGCCGAAAGAGGCCCGAAATGAAATCTATTTAATTTGGTTTACAGCCCGAAAAATGTATAAAAACATATATATATATACATAAATATATGTGTGGTTAGAGAATAAAAACGCGTGTAGTTGTATGCAGTGATAAGGCGGTGACGGGGACACAGGTACCAGGTGTCGAAGTTGGCACTAGGCTAGTCCGCCGCGCACCCTGACACCGTCCACATCGCCTGACGTCGATTGCAGAAGCCCATGTCGCAAAGCATGTTCCCAAAGCTGGCCGAGGATTACGCGAAATTCAAGCGCTATGTGAAATGGCTGTACACCCTCTACGAACTGAACACACAGATCGCCATATGTGAGCCGTGGGAAAAGGTCTTCCTCAGTGAGTACTAATGGACGGTGCCCTTTCCATCGAAAGGGAAAGATTAGCGCCGCGCCACCATTCGTATCTACGGAGTATCAACTTTAGCGCGATATATTGTCTTTACCCATCGATATCTTATCTATGCCGGTCACTCGG

GGCGGTCTTAGTCATGGAACCAGCTAATGGATTGTTATGTCGCAACATGCTATTTTCACTTTTAGATGTCCTCCTCGGCAGCTTCGTCTCCCTGATCCTCTACGCATCCTTTGCCTTTGTGCCGGGCTATTGTGTCACCGTCTTCCAGCTTTTATGGCCCCAAACGAGCGTGCAAAATCTCACCAGCGTTTGCAGTACGAGTACGGAGGGCTTTTGCGGCAACGAAAGCGGATCAGTAATAACATAATCTTAGTGTAGCTTATCTTAATCAATAAATAATTTAATACCTAGCGGGTAACGAAGTTTTTTTGAATTCATTTGGCGGTTTTCGTTTTGAATTTGTACAGCACTGCCAGGCGATAAAAGAATATGCAAGTCGCCAACCTTGGTGGAAAACCCCGCTTCCGATTAAATTTTAAATCGATTTAATGTCGATAAATGCAGTGAAATTAATCGATTGCGTCGCATAAACATTCATTTTGCATTTATCTTGTTTGTTTTCTTTATTGTGGTTTCATGAATTAGTTAAACCATGTCTAACGACAAGCGCACCATTTATGTGGGCGGCCTGGCTGACGAGGTCACAGAGAGACTGCTGAACAATGCCTTTATACCCTTTGGGGATATTGCAGATATCCAAATGCCGGCGGACTACGAATCACAGCGACACCGCGGATTCGCCTTTATAGAATACGAACAGAGCGAGGATGCGGCTGCTGCCATTGATAATATGGTAAGAAGTTGTTGCACAAGTAGATCAACCTGCAGCGGAAATACAACGAATCAACTATTTCCAGAATGACTCCGAGCTCTGCGGACGCACAATTCGCGTGAACTTGGCGAAACCAGTGCGCGTTAAGGAGGACAGTTTCAAGCCTATCTGGGCAGATGACGACTGGTTGCAGAAGCATGCGGGAGCCACGTTGCAGCCGGAAGGCGAACCGGAGGCAGAGAAAGTG

 

---

Search Again