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CRIMIC CR00790

Gene stmA

CG8739

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:8733144..8733171	44E3	FBti0200683	80661

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAAGCTCGACTTATCCCCTGAGCTT	GTTGGTTGTGTTGTGGTTGGTTTGGG

Images

Construct Design Sequences

sgRNA sequence	GGGGCAGCGCGGAAGTCGGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCTCGTTGATGTTGGCGAA	CGGAAGCCATCAACCTACGA	GAGCACCACCCAACTCCATC	CAACAACAACCTGTGCGGAG

 

Amplicon 5'

Amplicon 3'

TCCTCGTTGATGTTGGCGAACTTCACAAACTGCAAGGGCAGAGGAGTGAGTAGAGTAAAGTGAAGTGTTCCACTAAGTGAAGACTTACCGAATTGGTGGCCATTATCTTAAGGTTGGGGTTCGAGTCCTCCAGAAGCTTTTGCACCATCCGCAGGAAGGACTCAACAAACAAGTTGAGTGTGGTCTGGGCATGACAAGCCTGCAGAAGCAGGTCCATGGCCTCCATGGCAATTTCGGCTAGTCTAAACAAGAGTAAAGATTAACAAGCTGATTACGTAGCAAGTTCTTGACCCACTTACTTATAGCGCTTTCGGTTAATATCCTTGGTGGCCTTTTGGTACAGGTACTCCCCAATCCGGTCCAGCTTGTCCGGCGAGCTCAGCGAGTAGAAGGTCAGCTTCTCCATGTTCGACTTGACCAGCCCGTCCTCAGGGTTAACCGGGAAGATGTTATCGACCAAGCGCTTGTAGCGCGGCCTGAGCGCTGAGCAGCATCCACAGCAGCCTAGAAGAGCGCGGGGAAATGCACAGCGGTTAGAGGGATTAGGCAGACAAACGTGGATGCAGCGGTAGGTGAACATGGCGCCACCGCTCCGCTGGAGAGAGTGATGGTGGTGGAGGCGAAAGTGGTGGTGTGAAAACCGACTCGGGTCGACTGGGTGCGACCACCATTTTCCGCTTAATCTTATCGCGATAATTGCCCCAGTGGACTATAGTTTTGCTGTTTGAATGATTGGACGGCTCATCGCTCGTAGGTTGATGGCTTCCG

GAGCACCACCCAACTCCATCGATTTGTCGGTGTCGTCATTCCTCCTGGCCAATTGACTGGTGGCGAGGTCATCTGGCTCAATGGCCCGTGGCACGCCAAGCTGATGACACCTGAGCGCGCCGACAGCTTACCAGGCATTTTGGCGTCGCGATTCGCTTCTCTGGGTGTTTATCGCGGCATTAGATAATAAAACGCAGCGATAAAAGAGCGCAGCGCGACTTTTCTTTTGTTTTCCTTTCGTGGATGAGACACTCTCGGCCGACTTATCGATAGGCAAGAGGTGTGACCGTGCGATTAGTGCAGGCAAATACAGTGTAGAGTGCGGTGTAAGAACACTGCCATACTGTAAAATAATAATTGTATCTGCTAACTTGCAAATGATGCCCACTGTGCGGGGCTACAATGTGCTTTTTGAATTTCACTTCAAATCAACAAAAACTGGAGCTATTTTATTTTTATTTAATAGTTAAGAAGTAACAAATTAACTATAAACCGTAGTCAACCTTTAAACTATTTTAACATTTAATATTTCGTACATGTTTGCCTTCAATATTCTTATTTTATTAATTTATATAAATATTTTTACTTTTAGAGAAAATCTCCTTCATTATATGTACTTACAACTGGGATCCGTGCACTTTTGCACGAAGCTGTCGAAGAACTCAGGCAGTTCCGGTGGCTCGAAGCAGCAGCGAATCAAGGCCATTTCGGGATGCCAATATCAGGGATTCAATCAGCCCGCTCCGCCCAGGACGTTGACCATGAACTTTGGGTATTTTTAGCGCGAAACTTTGTGCCTCCGCACAGGTTGTTGTTG

 

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