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CRIMIC CR00794

Gene CG8858

CG8858

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12198690..12198712	48E8	FBti0200687	80665

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCTCCCCTGTGTTCAGGTAAACAT	GGGGACCACTAGGGTAACAAAAACGT

Images

Construct Design Sequences

sgRNA sequence	CTGAAATCGAAGGATTGGGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGGAGTGGCTACGCTGAAA	CCTTCGATTTCAGAGCTTCTGG	TGGCACAGCTTTGGTGTATT	GGCCTCCTACTAATCCGTGC

 

Amplicon 5'

Amplicon 3'

CTGGAGTGGCTACGCTGAAACGTGTGTCCGCGGAAGCCACCACCAGTGGCACGAATATCTGCAAATCACTAAACACACTGGCGCACAGGAAACGAACTATTCCCTTCTTGACCTTTTCCAGTTCTTCGGCCCGCCAATTGTAGGCGATGATGCGTGTAAAACTGTACGGGCTCAGACCCGGAGGCACATCCTGTTCCTGGGTGATGCCATAGGGCAGGAGGAGCACATCCTGGAGCAGTGCCAAAAAGTTTGCACAGATGGCTGGCTTGTCCTGCAGATCAAGTAGCTTTGAGCGCTGCGTCGGGTCGTCGGGAATCTTCATGTCCGACAGTACGGGCAGCAGCAGGGCGAACAGCTTGTCCTGGTAGCTCTCCAGCTTGTCCTCGCAGCCCACCACTTTGCTGGCCAGCGCAGTCTGTTGTTCCAACGCCAGGCGCGGAAAGCCCATGGTAATGAATATGATGGCGAAGTTCTTGAGGAAGGTGCTGCTATCCTGGGCGGCATACTGATCCAGCAGTGCCTCTACGGGTATTTGCACATGTCCGCGCGACGACAGGCGCCTCTTGATATGGGTGAGCACTTCTACGACCTAAACAAAATTTAGTGCATTAGTCAACCGGTCAATTTTGCAATGTCACGATGCGAGAAGGGTGTACAAAACCACCCAGCCAATCGTTGTTTGACCATCCACTTGCCTTTGTGCGCACCACATTGTGGGGCGAGTTCATTTTGAGGATCACCGGGGTCAGGAACTTGCCCACAGCGGCTTCCAGCTTCTCATCCGAGTCCGCATTTCCCAGACGCAACAGGACGCGCTCCAGAAGCTCTGAAATCGAAGG

TGGCACAGCTTTGGTGTATTATCAGCCCATCTCTTCTATTCGCTGATGAAACATAAAGTTTGATGCACACAATGCTAATTATAATTTTTACCAATTTCTGCATTCGGTCCAGTTTCCATTTTTTTTGCTTTATTTTTGTTTCCCTCTTTCAGTGTTACCAGCTGTTGTGCTGAACATTTCACAGATGCCAATTTTTGGCTACATTCGAAAGCTTTGCAACACTCGAACTTTTCGAAATGAAACCGCCGCCAAATTCAAAATGTAAAGCGAAAATCTTTGCCATTGAAATCATTCAATAAAAACATAATTTGTAATCAATCAACTTTTATTTAAGAAAATCATATTAATAATGTACATTCAATGCAATTCGTGTCCATATTCTTGCGGTCATCTTTAGTTTCTGTAAAAAATAAGAAATCGTGTCATTAGTATGGAGTATTTGCATTTATTTCAATATGCTTTTCTTTGATAAATATCAGTATTCTCATTTTTATTGAGAAGAGTCAAGGTTCTTCAGCAATTTGTTTTATGAAGACCGTCATGATTTTATCATCAGAATGCCAGTTAATAGCTGCCTTACAAAATAATATATTTTACACACCTTTTAATGGAATAGCTGGCTGCACGACTGCTGAAATGCTGTGGAAACTGAGAGAAGAACTGGAGAAAACCCATGGTTAAAGTTACTTTTTTTTGTTTTATTCAAAAAGAATAATTGCTACAAATATTATCGCCCGAATGGTAGTTGTCCGCTCTAGTACTTCTTGAAGCTCTTCTTGGCTCCCTGACCCTTGCTGACTTTCAGGACGTTGAAGCGCACAGTCTTGGACAGAGGACGGCACTCACCAATGGTGACAATATCGCCATGCTCAACATCTCTGCAAATTAAGAAATAGCACGGATTAGTAGGAGGCC

 

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