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CRIMIC CR00795

Gene HSPC300

CG30173

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24028583..24028584	60B4	FBti0200688	80666

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCGTACTTCTCGCCCTTTTTCTTG	CCCGGTTCCGGAAACTCTTGGTTATA

Images

Construct Design Sequences

sgRNA sequence	CACATGCGTCAGAGGGAAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTTTTTCGTTCAGCACCGC	CCCTCTGACGCATGTGTGT	AAGTGGGTGCTTCGTATGCA	GATTGGAACACGCGGAATCG

 

Amplicon 5'

Amplicon 3'

AGTTTTTCGTTCAGCACCGCCAGACGGGAGCGACAGGACATATCTGAAAGGATATGATAGCACACGATGATACAGTTTATAATGCCAACAGTGTACATAAACCCTATTAAGGATATGGGATATTTTAGCAACCCAACTACCTCGCACTCTTTGGCTTATCATTTATCACAGTATTTAGCGTATCTTAAATTATGCATAGAGCAACCGAAAAAGCTTATCCAATTCATTTAGGTGACTAATTCGGATAGAGATAAAGCCGAATTTTCGTTTTGAGCCCTCCGATGATGTTACATACATACATTTCCATATGTATGTAGATACATATATTGCTATACATATATGCCACCACGCACAAACCACTGTTTGTTTCTATAGACTTAAGACTTGAGTAAATAAAGTCATTGTCACTGATGAATATTTGTTAGTAATATTACTTATGCGTTCACCCACACCTACAAAGGAAGTGATAATAAACACGCGCACACACACATGCGTCAGAGGG

AAGTGGGTGCTTCGTATGCAGGTTGCTTACCGAAAGAGTTCAGAAAGTCGGTGATTCTCTTTATGCTGGCAGTTATCACTTCGATATACTCCCTGTTCGCCCAGTCCTGGTGGATCTGCTTTTGGATCGCCTCTCTGTGAGCCCCACTCATCTTTATTTATTCCTATTGTTTCTGTTCGTTTTAATTGTGTTTATCTAGAGATGAGAGATGGGTTGGGCCGGAACAATTATTGTCAAAAATTTGGAACTATCGATAGGCATACAATTACCGTGTTCACCGTGATTCATTGAGATCAAATTTTTACTCCAACAAGAAATTTTAATGGTGTTTTACAACACTCCCTAAGCACTACTATTTAAAAATTACGCGGGAAAGTGCGATGAAAGAAATAACTTAATATTTTTGTCTGCCAACTATAGCGTGTAAATATGAATAATAAAATAAAGGTAAATAATAAAAGTATTGGGGTAGACACAGCCGAATATATAAAGAAACTTATCGCCGCAATATACAATTGTATATCGCAATATACATAGATATATTCGAATTTGCGTTTGCTATATTTTATATTAACCGTGGGTTAGTTTTCGATGTTTTATTGTTTTAAATGATATTTATTAAGGAATTACTGCAAACCAGCAAAGTATGTACCCATTTTGCGGTCCGATTCCGCGTGTTCCAATC

 

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