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CRIMIC CR00796

Gene CG8401

CG8401

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:16029673..16029691	52D15	FBti0200689	80667

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGTTGCTCTTGGGCCTGAAATACG	TTGGAGAAGGAGCAGGCATGTCCAAT

Images

Construct Design Sequences

sgRNA sequence	GTTCAGTGGGCCGGTGATGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTGTCCAGGGGGAGAGTAT	GAACCCTGCTCACCTTGCTC	GGTGGTGGTGATGCTGCTAT	GCTCTCGTCCTGCTGCATAT

 

Amplicon 5'

Amplicon 3'

GGTGTCCAGGGGGAGAGTATGCTTGCTACAAAACTAACTACGTATTATTGTTTATGCTAATTTCTGGTTAGTTCTCAGCATTGCCTTTCAGGCGAATGTGTTGCTGCTGCCTCCCTCGCTGAAACTGGGCGAACTGAATGCCAATGGGGAGTACGAGCAGCTGGCCGAGGTCTACCCGAAAGTGGTCAACGAGGGCCAGCTGAACAGGACGCAGTCCGGTGAGTTTGAGTTCATATATTATATATAGCATATATATAATATATATCATAATAACAATACATTCATTGAACCTATCGTTTGATTTACCTTTGTGTGTAGACCTCTGCCTCCGGGACATTGTGGAACTGAAGCCACCCCACTGCAAGCTGCTCTCCGGCGAATGTTTGGAACTTTTGGCCAGTGATGCACCAGCCTACGGATACCAGAGAACCCATCAGTAAGCGTACATATACATATATCCACCGCTTAAGACATCTAAAGCTAGACGATATTTCCATCTAGGGTCCTGCTGCTCTACGTGCTGGAGCATCAGGCATGTGCCCCCCACCTCAGCCCACCTCAGGTTTACGAACTGCTGGCCAAGAGTCAGTGCGATGAGGTGGAACGCGAGCAGAACGCCATCCGGAGCCTGGGGCTGCCAGTCGTGTATCGCGATCTGTATCTGGAGCAAGGTGAGCAGGGTTC

GGTGGTGGTGATGCTGCTATCGCTGTTGCAGCTACTGCTGTTCTATGGGCTATCCTTGTTGGGCTATCGCTGACATATATCTTTGCCTTGCCACATATCTCTGACATCGCAGCCACCATCTGCGGCCTGTTTGGCTACAATGAGTTTGTCAACTGGCGCAACGTAATGGAAATTGGCAGCTGGCTGGAGGGGGACTCGCCGAAGGATATGGATGTATTGGCTGACGACGGCGATAGCCAGCACATAAGCGATTTGGCCTTGGTCTTCTACACAAATGCACTGCTGCTGCTGCACTAATCCATCGAACATTCCCCCAATACCAAGCCCATCCATAATTTCTGCTTCTGCTTGATAAGTTAATAAAGTGGCGCTTATTGCTTTATGGTTGTGTCGATTTCAGCAAGTGTCGTGGTTAATACTTTTGTTATTGAACTGCATCGTGGTGCAGTGATGCGGGAGGGGTGGTGGTGGTGTACTTCGCTGGGAACGGAAACAACATTTGTAATGTGACACATTACTTGCGCTTGGTTGCTCGCCTGATGGATATGCAGCAGGACGAGAGC

 

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