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CRIMIC CR00801

Gene AsnRS

CG10687

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:19131748..19131761	37C1	FBti0200694	83195

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTAAAACTAAACCACGCTGCGCGCCT	TCTGAGGCGGTTTGAAGTCAGGGTTA

Images

Construct Design Sequences

sgRNA sequence	GGCAACGTGGACCAAATAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGGCGTCACCTCGTTATAG	TTGCCAGCGAACCAGATGTA	AGTTGGCCCAGTTCTATCACT	GATAACGGTGGCACTCGGAT

 

Amplicon 5'

Amplicon 3'

GGGGCGTCACCTCGTTATAGCCACGCGCCTCGTAGTGGCCACGGAATGCTTGCGTCACAACGGATCGCATCTTGAGCACTTTCGAGGTGTTCTCTCCACGAATCATGATATGCCGGTTGTCGAGCTGCACGTCCGGTTGTGCTTCCTCGTTGAGAATGGCATCGGCACCGCCGGGCGGAGCCAGACCAATCAGCTCCCAGTAGTCCACGTTCAGTTCATGCCCTCCCGGCGCCGTCTTTCCTTCTGGAACTAGTTTCAGGGTACCGAACAGGACTACAGAGCTTTCCGTGCTCAGGGTGAGCGCATCGTAGGTCTGGCAGAGCTGATCGTTTAGCACGCACTGCAGGAAACCGGTGCCGTCGCGCAGTGTTATGAAGATCAGGGACTTGCCCTGACGACGCAGACGATGCACCCAACCGTAGACCTTGACACGCGAGTCGCGGTTGGCAGTGCCCTCGTAGATTCGAATTTTCCGGGCCACCGGCAGACTGGGATCCTCCGAGATCTTCACCTTTCGAGCATCCTCCAGATTCTGCTGGCGCTTCTCCGCATCCTCCGCCTCCCGTTGCAGCTTATCGGCATTCTTGTGCGACTCCCTGACGAACAGTTTCTGAATTTTCTTCAGCTGGGACTTGGCAGCCGGCTCGTAGGGCTCCGCTGCATTCGGATCCTTGGTGTCTACGTAAATCGTGGGAAAGGGTTCCTTGCCCGCATGACGCATCGCCTGCAGGATGGTCTTGAAGGGCTTGGACTCACTGCCATCGCCGGACTCATCACTACCCTTCTTTTCCGACGTAAAGATTGCACCTGCATATGGTCGTACATATCAACATTGCACATTATGAATCGATACATCTGGTTCGCTGGCAA

AGTTGGCCCAGTTCTATCACTTAAAAACGTGCTTACCCAAGGTTAACTCAGCCAACTGATCGGCGGGCATCTTGCTTGCTTTGTGACTAAGAGTTTACTAAGAACTGAACTTAAATATCGCTAATATACGCACAAAATTGCATCAGCTTCAAGTTGACAACTTTGGCAAAACGATACCTCTCGCCGGATACTCGATAGACACGATGCAAAGCAAGTGCTGGAAAACGCCGATTGTCAAGTGGGGGGCTGAAAGTTTAAAAATGCAATAATTTGATTTTTGGACGGGAAATCTTCTATTACATTATAAATAATTGTGTTTATTTCTTAAAAAATATTTCTCTGCATAAAAGGGTTTTATAAAAACAACTATCCCTATGAACGGGAATCAGTATGTATTGTTTATAAATGAGTATTCAACATTATTTTGGTTTTTGAGCAATAAAGTTCGACGTCATTATTGTTATATCTGGTTTTTTTATGCAAAGGTCTTTAATCATTATTTTTACCTTTCCGATATTTTAAAGTAACTAAATCTGGCTATAATATATCGCCTCATTTGCAAGCCCTCATTTATTATCGATAAATATAAATTCCTTCTCACCTTCGTGAGAAAACGTAAACAATTTAAGAAAACAGAAAATGCCCTCGGAAAACAGGTGATTGCAGCATAAGTACTTGCAAAACTAATTTACGAAATGCAGCTATACTATTGCCACTCAACAGAATCAAAATCCTACCCAAGGCGGTGGTCTGCGAGGAGAGCAGCCTGCGCGGTGACATCACCTTCTCGTCGGGCTGTGTGGTTCATCCGAGTGCCACCGTTATC

 

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