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CRIMIC CR00802

Gene jing

CG9397

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:6615873..6615886	42B2	FBti0200695	80672

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAATCCCAAATGAAGCCGTCTCCTGG	ATGAGAGATCAGCTGCCGAACTAACC

Images

Construct Design Sequences

sgRNA sequence	GCGTTTCCTTACAGATCGGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGCAGTGGATCTTCAGGCA	AACGCAAGCTTATTGACCGA	GGACGGCTTTGATTCTCGGA	GACAGGGTGCAGGAGTTTGA

 

Amplicon 5'

Amplicon 3'

CAGCAGTGGATCTTCAGGCAGCTCCTCGTCGTCCTCGTCTAGCTCCTCAAATGGCTCGAATACGGGAAAATCGCCAACGAAGAAACACGTGGCCATCGCACCCAGGACGCCGGAGATGAAACAGCAGCAGCAGCAGGGAAAGGCGGCCATGGTCTACCGACCGCCAGTAACAAGCACCGCTTTGAAGCAAAAGCAGATCTCACCACCCGCCCCCGTTGCGGCCCACACACCCGCCCCAGCGCCAGCATTAAATCCAACGCCCGCCCCCGCCAATCCCACGCTTTACCAGCTGCCCGTACAGCTTCCTAATCTAGTCCAGCTTCCGCCTCAGCTGGCGGCTGCCGCCAATATTATGCAGCTTAATAACGTGGCCAAAGCTGCAGTGGCTGCGGCGGCCACCAACAACGCGGCCGCGCAAGCGGCACAGGCTGCTCAGGCAGCTGCTGCCCAGTACTTCCTGAACGGCACTGTCTTTAAGCTGCAACAGGTGACTACGGCCACGACGACGACGGCCACCACGGCCACAGCAGCGGCGGCCCCAGCTGGCAATCCCTTTGGACTGCTTAACTTAGCCACGGCGGGCAATCCCTTTGGCTTACCCAATGCCAATGGGCAATTCCCCATTGAAGCCATTCCGGGAGCTGGAAGCTATCTCCATCATCAGCTGCTTTTGGCCAGACAGAACAACATGAGTTTAAACGGTAAGTATAGTATATTTTTAGTTTAATTAAATGAACTCCGAAAGTAGGAATAGCTAAGCTTCTAGTTCGACATAACTTGCAGAGGCATAAAACACTAAAGAACTCGAAAAAGTCGGTCAATAAGCTTGCGTT

GGACGGCTTTGATTCTCGGAGTTTTCGCTTATGCTTACTTTTCCATTACAGAAACTATGGCTTCCAATTCTTGCGGCAACATGAACTTTGTTAATCCACTTAACAATCAGCAAGTTGGACTCAAAGATAACAAATTATATATTGTAAACTCGGCCGAGTACCTAGGATACTTAATGAGTCTGCAGATAGCTCTTAATAACCAACAGCACCAGCAACAACAAATCATATCTGCCACACAGCCGCCACCACTGGCAGCCACAAACAATAACAGTACGAACACTGCCACTCAGCCGCCGCCATTGGCAGCTACGAACAATACCAATTCGAACACTGCCAACAATTCGACTGCCAGCAACAGTATCAACCATAATGCCTCGAATAATCTAAGCAATATCAACAACACTGCCATACAGCCTCAGCGGGCGATTGTGAAGCCGCCATTGCACAGTTCCACGCAACCGCCGCCGCTTGTGACGATTTCCTCCATGCCCGCGTGCTCACCGGCTGCCGCATCTTCGCCAGCGGCCAAACGGTCACTGCCCGCCGCCAAGCCTTACCAGAAGCGGCTGACAGCAAAGGGCAGAGTGGGGACAGCACCGATAATCGCAACGCCTACAACGCCGCCTCCTCTGGTACCCACGTCTGCTACTAAGGAGCTGGGTCAGTTGCGCAAGAGTACGGGAACCACGGGAACCCCTACTGGCACACCCACACCAACGCCACCGCTAGTAAGCATCGCACCCTCGAAGCTGACCCCAACGCTGAGCGTTTCGAAGCAAGGACCGACAATGAAGCTTGCAAACAGCGCTCCCGATCTCTTTGACCTGGTGAAGAACTCAAAGCTGGTAGCCAAGGTTTCGCAACCGTTGACGCCGCTTCCCTTCAGTTCCCCCAGCAGCAGTAGCAACGGAAGCCACGGTAGCCACGGTATGCGATCCTCGCCGGCCCTCTCCACGTCAAACTCCTGCACCCTGTC

 

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