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CRIMIC CR00805

Gene RYBP

CG12190

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22671325..22671341	58F7	FBti0201837	80674

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCTCTGTTTGGTCGGGTAAGTTGAG	GTGTGCGGACCTCTCTATATCTGGTT

Images

Construct Design Sequences

sgRNA sequence	GCTTTCTTGATCATGTGTCATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCAGCACCATACGCTAGCC	GCGAAAATCGGAACATGGC	TCATGGATCGGTTTGTTTATCCT	GCGCTGCTAGTGGAACATTG

 

Amplicon 5'

Amplicon 3'

ATCAGCACCATACGCTAGCCAACACTGGCTGCGCGCTACTATCGAAATATCGATAGCCTGGCATTGAGCACTTCTACGATATGGCAATGTTGCCGCAGCCAAAAAGACAATTGCAGAAAGTTTTTTTCACAACAAAAAGCCACCGAAAACGCTCCAATTAAGCAACAAATGCGTGCCAAAAGCGCAGCGAATCTCCACTGAGGCCACCGTGTGTGCGTGAGCGTGGCAAAGGTGCGAGCAGAGCAACCAAACATCACAGGAACACGGCCAGCAGCAGCAGCGGATCAGCAGAGGGGCGGGCAGAGGCAGAGGCGGTGGCAGAGGGAAAAGTCGGGCATTTTCATTGGATTCGTTTTGGTCCGATTCGATTGGATTGCGGTTACTCCAGCGGCGCCCAGGCGTTCCTCCTCTCCTCCTGTATTATGGACAAGAAATCCTCGCCGGTGCGACGACAAAAGCGGCAGGCGAAGGTGATCGAGGAGAACTTCTGGGACTGCAGCGTCTGCACATATAGAAACTCGGCGGAGGCCTTCAAGTGTCGCATGTGCGACGTGCGGAAAGGTAAGTGACCCCGATTTTGCTTCGTTTTCGCTTCCGAGTTCCAAGATTGAGCAGATTGCCAGCCATGTTCCGATTTTCGC

TCATGGATCGGTTTGTTTATCCTTCTACAAGGATACACACACATCGGTATTAACAATATTGGAATATATCTGGTTGAACTGTAGATAGTAATAGTCAAAAAAAGACTGTTGACTGTTGATTAGATGGCCAACGATGGTATCGATTTGCTTACGTAGAATAAATAAATTCACACTGCGCAGAAACCGGGAATACTTCTCGAAACGATAAACATGCTAATTGAAACAATTTTAAACTATTTATATACTATTTTTGGTGCGCTCAATAAGACTATACACCCTTGTTTTTTAGGAACCTCCACGCGAAAGCCACGCCTGAACTCAGCGCTAGTCGCCCAGCAGGCCGCCACTTTGCCAGGAGCCAGTGTCAACATGCCCAACGGGAAGTCCGCCTCCGGATCACGGCATGGCAGTGGGCACGACCGGCAGAGGCACAAGCGGTATCCGGCACGGTTGAAAAACGTGGACCGATCGACGGCACAGACGCGAGAGGTGACGGTCAACTCGGTGACCGTGTTCATCACAGAGTACAAGGCCAAGCCGGTGAGCAGCCGACGCGAATCCAGTGAGCAGAGCTTCAGCGAGAGCAACGACAGCCGGAGTTAGAGGAGGGATACAAGGCCTCCTCGCCCCCAGCTCCCCAATATTCTACGCCACGGTTCCCGCTCCCCTGTTACAAGTTGTAAATTCTAAGTGTTAGGCCCCTGGCGATAACAATTGTAAATCGAAATCCATTTAAGTTACCAAGTATTAGGCCACGTTTAAAGCCCCGAAAGCCAAGCCCCCACTAGTTCATGACTTCCAGCCCAGGCCAAGATGGCAAAGCAAAGCGAAGCGACGGCGAGTCGATTGCATTTACACGACGCTCTCCAAATCCCATTTTAAAATGCGCTAGACCCTGTAAATAGCGATGCATGAGTTCCCTGAATATCTCTGTCTCGTATCGTAATTTTAGTTGTAATTTAATTCTGCTACTCTAATTCCTAATTTATTGATGCAAAGGTAACTATGTATAACCGCCGTGTACATGTTCAACAAAGATATCATTTGTTTTGTAAGTGTCGAATTAAAATATGGTTTAACTAAGTGCAAGTTAATCTCAATGTTCCACTAGCAGCGC

 

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