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CRIMIC CR00806

Gene levy

CG17280

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23548461..23548461	59E3	FBti0199622	79295

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATCTGCGTTGTGTCCCAGTTCGTCA	GGTGTCGTACAGCAACTATAACGACC

Images

Construct Design Sequences

sgRNA sequence	CACCAATCGATCTTGCCCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTACCTTTGCAGGCCGAGA	TCCACCAATCGATCTTGCCC	TGGTGGAGAAGGATTCGCTC	CCACCTAGACGATAGCGACG

 

Amplicon 5'

Amplicon 3'

CTTACCTTTGCAGGCCGAGATGAGAAAGTAGCCGGCGTCGCAGATGTCGCTGAAGTCCAGGTGGACCACGGGTCGCGTGTGGCCACTGCAGGTCAGCGGGATTTGTCGCAAGTTGTTGGCGGCCATGGCAGTGGGCTGCTAGTGGATTGGTGGACTGTGTGGTCCTTAATACTTGTGGTTTTCTCCGAGTGTAGCGACCGTTATGTCCGCTGGCGAACTGTGCACTTAGTGAATAACTGACGTTTTGTGGCTACTTTGTACTTGGATTGGTTGTTGCTCCTGGTTAACTGCGCTTCACTGCCCGTACGATAATGATTTTCCCGTTCGTTGCTTAGGTTCGAGATGCTGCGGTGGCTTTGCAGGGTGTGCAGTGTATTTACAATCTGATGCACTGAATTTTATAAACAAGAGGGGAAAATAATTACGCGACACGTTGTTGTTGTGGATGGACGATTGGTAGCCGACTCCAGCCTCGATAGTCCGCACACACAGTACCTACCTATCGTCAGCGGTGGGCGCGTGAGGCGATAACTTCTCGATTAGCTAGAGCTGTGTCACGGCAGCCAGGGGAGGATACAAAGGAAAAATGTCAAATTACTTATTTACAATTTGGCATAGAATTGTTTTATTTTGTTTTTTATTTATATTAAGCCAATTAACAGCTTTGTTGTACGTAATTTAGAAATCACGGCCAACCACTATCGATATAATTTAGCGAACGAGTCACGACATTATGGATGTAGCCCATCCCTATTTACTTGTCAAAACAGCAAATTTTCATTCGGGCCAAGAATCGACTGAAAATATGTCCGCTATTCTAAACCACGCAATTCGCCGCCAATTCGGCGCTTCTGCCGCCCGGAATATGTCCGGCACCGCCGCCGTGGCCGGCGAGCACTCTGGTAAGTGACCGTCCAGCGTCCCAATTGCCAAATTCATCCCCTTTTGTAAGGTTAAGGTTATGTAATTTACCATTGGGCAAGATCGATTGGTGGA

TGGTGGAGAAGGATTCGCTCTTATAACACATCCTTTCATGCTCATCTCATCTCTCATCCTTCAGGTGGCTACAAGGTGTGGAAGCGCCTGTCCTTCTTCGTGGCCGTGCCCGCCGTGGGACTGTGCATGCTGAACGCCTACCTGAAGCACCAGGAGGAGCACGACAAGCCCCGCCAGGAGTTCGTCAAGTACGACTACCTGCGCCGCCGCGAGAAGCGCTTCCCCTGGGGTGAGGGCCAGAAGAGCCTGTTCCACAACCCCCACGTTAACGCCCTGCCCGACGGCTACGAGCACTAGGTGGCCACCGTCTCCGGTTCCCTCTGCGGGATGTATTCGTTGTTTGGTAGTGATAGACGTTAATGTAAACTGAACTTTACGCGGAAATACACAGAAAACACATATACCCAAACTCCAGTTGGCGCTTCAATAAAGATCAAATTAGAGCGAGGAGAGATCCCATAAAGACCATTTCAATGAGCGACAGCGGAATTCTGGTGAGGAGTTTATTTAAAAGGCCTTCGGTTAAAGGAAAAATTAAAAGTTACAATTAAATAGAATTTAAATTTAAGCATAAGCACGGATGCACTTTTCGCATGCAATATATTCAACTACTATCATTATTTTGCGGATCGGCGATTCTTAAGGTTGCGTTCCAGTTTGTGATGAGGCTTGCCTTCGTTCTTTCCTGTCTTCTGAGTGATTCTTTGAGCATCCCGACTGCGAGTTTGGCGCACGGATTGCTGGGCATCCTTGGGAGGATTGGTTTTACGCTGCTTTGGAGGCGGCGGCGTGAAGGATTCGTCGCTATCGTCTAGGTGG

 

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