CR00808

CRIMIC CR00808

Gene CG9945

CG9945

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:20620374..20620374	57A7	FBti0199623	79296

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGCCTGATGTCGCTGGAATCCAAAA	CCGCCTCGGAAGCTACTGAAAAGAAA

Images

Construct Design Sequences

sgRNA sequence	GGAAACACAGAAATGGCCTTTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATTTACGATGGCGCCAAGG	AGAAATGGCCTTTGGTTTCAC	GCCATTTCTGTGTTTCCGTTGA	GTCCTTACGATGAAACGCGC

Amplicon 5'	Amplicon 3'
CATTTACGATGGCGCCAAGGGCACTTATCACCTGCTGAATCGCATCCGAGCCCGCGATGTTGAGTGGAGCATCATCGATGCGGATTTTAGTCCGAATGGCGAGCACTTCGCCTACAGCACCTGGTCCCGCTCATGTAGGTACTCCGCCCAATCTGTTTATCCACACTAAGTTGTTTTCCCCTTCAGTGTTCATCATGCCCGTGAATGGGGGGGAAGACGACTGCCAGTGGATCGATGTCAATGGACTGCCAAGCCATAGGTTGGCCATCTTCTCGCTGCGCTATTCGCCAACAGGCGACAAAATCATCGGGGGCAGCAACAATTCCACGGTCATCGTGACCGACATTCGCACAAGAAACACACAGATCCTGCGCACCCACCGCATGCCCGGCAAGGATGTTAACTCGGTGTGCTTCCTACACGATAAGGATCCAAATGTGATAATCGCCGGCTGTGACGACGGCCTGCTAAAGGTCTACGACCTGCGCACCACCTTCCGTTCACGGGACCTGTCCAAGTCTGTGGCATCCTTCATCGGCCACTACGATGGCATCACCTACATCGATTCGCGCAACGACGGCTACCACGTGCTGTCCAACTCCAAAGACCAAAGCATCAAAATCTGGGACATAAGGCAGCCGAGCAATATGCGTAATCGCAGTCGTTCTAGACAGCAAGTCGACCCCACCACATGGGACTACCGATGGAACCGCGTTCCGCGGGAATGTAAGCTCGTGCCCGTTTTATTCTTGGAAAATTTTTAACGCCCACCTATCTTGTCGGCAGTTTACAATCCGCATAAGCCACTGGAAGGAGACTCCAGCATAATGACCTACCGCGGTCACCGAGTTACCAAAACCCTGTTGCGGGCGAAGTTCTCACCCATGGAGCAGACAGGGCAACGCTACATTTACACAGGCTGTGCCACCGGACGTATTATAAGTGAGTTCACACCTTTATCTTGCAGTATTTCTCTGATTTTCTTTCACAAGGCTATCTAAAATACATTTTCTAGCCAGTAAGTTCGCCAAGTATTTTTAGAAATTCTCTATCCATCAAAAATTTGGTTGTGAAACCAAAGGCCATTTCT	GCCATTTCTGTGTTTCCGTTGATAAACATATTATGTTTTCAAAATAATCCAATTTTCTTACAGTTTATGATGTACTTACTGGTAAGATTCAAGAGGCTATTGAAGGACATAGGACTGTGATAAGAGATCTGGACTGGCATCCGGATCGCTCAGAAATCGTGTCTGGCTCGGTAAGCATCGAAATATGAGTAATATTCCTCTGAATAATAATGCACACTCAACTGCCTACGCAGTGGGACACCCATGTTCACCTGAACAACTTCAGCCGCAGCAACGCCAATCGTCCGGTAAAGCGATCACATAGCTCGGATCATGACAAAAAGCCCCTGAGAAGATCACGACGTTTGGCGAATCGAAATGTTACACCGGACTAGTTGTAAGGTTTAGAGTATAAGCTAGTAAGGTCCCCTTTGATATTACATTTCGTTACATTCATTCATAGTGGCTTGCCTAAGCGACGCACGAGAATATTCACCACAGTCGGGCCGGCTGGGATAATTGGTTCTATTAAATAAGACTGAAGAATATGTCACTCACTTGAAATGTATAGTTCAGTTTAATTAGTTAGCGTGTTATACAAAACAGTACATTAAAATGTAAACCATGAAGCTTACCAACTACCTACTTAAGTTGTTTATGTACAAAAACAATCATAATAAGAATCACGTTGAAATATATTTCGTTTGCTCGATCTAAATTCCTTGAAAGGCGACTCTCTTCTTGACCGCTCGTATGCTAGTTCTTTGCACCCTTACCGGATACTTGCTATGCTGTTTGCTTTTAAAATGCTCAACGGCTTTGTTTTTCCAAAGGTTGCGAATGCGCGTTTCATCGTAAGGAC

Amplicon 5'

Amplicon 3'

CATTTACGATGGCGCCAAGGGCACTTATCACCTGCTGAATCGCATCCGAGCCCGCGATGTTGAGTGGAGCATCATCGATGCGGATTTTAGTCCGAATGGCGAGCACTTCGCCTACAGCACCTGGTCCCGCTCATGTAGGTACTCCGCCCAATCTGTTTATCCACACTAAGTTGTTTTCCCCTTCAGTGTTCATCATGCCCGTGAATGGGGGGGAAGACGACTGCCAGTGGATCGATGTCAATGGACTGCCAAGCCATAGGTTGGCCATCTTCTCGCTGCGCTATTCGCCAACAGGCGACAAAATCATCGGGGGCAGCAACAATTCCACGGTCATCGTGACCGACATTCGCACAAGAAACACACAGATCCTGCGCACCCACCGCATGCCCGGCAAGGATGTTAACTCGGTGTGCTTCCTACACGATAAGGATCCAAATGTGATAATCGCCGGCTGTGACGACGGCCTGCTAAAGGTCTACGACCTGCGCACCACCTTCCGTTCACGGGACCTGTCCAAGTCTGTGGCATCCTTCATCGGCCACTACGATGGCATCACCTACATCGATTCGCGCAACGACGGCTACCACGTGCTGTCCAACTCCAAAGACCAAAGCATCAAAATCTGGGACATAAGGCAGCCGAGCAATATGCGTAATCGCAGTCGTTCTAGACAGCAAGTCGACCCCACCACATGGGACTACCGATGGAACCGCGTTCCGCGGGAATGTAAGCTCGTGCCCGTTTTATTCTTGGAAAATTTTTAACGCCCACCTATCTTGTCGGCAGTTTACAATCCGCATAAGCCACTGGAAGGAGACTCCAGCATAATGACCTACCGCGGTCACCGAGTTACCAAAACCCTGTTGCGGGCGAAGTTCTCACCCATGGAGCAGACAGGGCAACGCTACATTTACACAGGCTGTGCCACCGGACGTATTATAAGTGAGTTCACACCTTTATCTTGCAGTATTTCTCTGATTTTCTTTCACAAGGCTATCTAAAATACATTTTCTAGCCAGTAAGTTCGCCAAGTATTTTTAGAAATTCTCTATCCATCAAAAATTTGGTTGTGAAACCAAAGGCCATTTCT

GCCATTTCTGTGTTTCCGTTGATAAACATATTATGTTTTCAAAATAATCCAATTTTCTTACAGTTTATGATGTACTTACTGGTAAGATTCAAGAGGCTATTGAAGGACATAGGACTGTGATAAGAGATCTGGACTGGCATCCGGATCGCTCAGAAATCGTGTCTGGCTCGGTAAGCATCGAAATATGAGTAATATTCCTCTGAATAATAATGCACACTCAACTGCCTACGCAGTGGGACACCCATGTTCACCTGAACAACTTCAGCCGCAGCAACGCCAATCGTCCGGTAAAGCGATCACATAGCTCGGATCATGACAAAAAGCCCCTGAGAAGATCACGACGTTTGGCGAATCGAAATGTTACACCGGACTAGTTGTAAGGTTTAGAGTATAAGCTAGTAAGGTCCCCTTTGATATTACATTTCGTTACATTCATTCATAGTGGCTTGCCTAAGCGACGCACGAGAATATTCACCACAGTCGGGCCGGCTGGGATAATTGGTTCTATTAAATAAGACTGAAGAATATGTCACTCACTTGAAATGTATAGTTCAGTTTAATTAGTTAGCGTGTTATACAAAACAGTACATTAAAATGTAAACCATGAAGCTTACCAACTACCTACTTAAGTTGTTTATGTACAAAAACAATCATAATAAGAATCACGTTGAAATATATTTCGTTTGCTCGATCTAAATTCCTTGAAAGGCGACTCTCTTCTTGACCGCTCGTATGCTAGTTCTTTGCACCCTTACCGGATACTTGCTATGCTGTTTGCTTTTAAAATGCTCAACGGCTTTGTTTTTCCAAAGGTTGCGAATGCGCGTTTCATCGTAAGGAC

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