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CRIMIC CR00812

Gene yki

CG4005

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24066317..24066352	60B7	FBti0199624	79628

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAATCTACGCAGTGGGAGGATCCCA	CTCCCCAGACGAGACTGCTCTTATTT

Images

Construct Design Sequences

sgRNA sequence	CAAAGATACACGGTAAGGGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAATCGCGTGCATTCTGGTC	GCAGAATCTGGGCTTAGCG	AAAGGCGAGGCATGGAGC	TTCGGGATTGGGTTGGAGTC

 

Amplicon 5'

Amplicon 3'

GAATCGCGTGCATTCTGGTCCAAATGCACGCCGCATTCTGGTTACTTGGTTACTTGGCTTGCACTTTACAATAGTCGGGGTATTCGGGTTTCGGCATGAGTCATCGCTGCTGACCACACCTTTCATCCACCTGAACTCTCTCTTCGGCGTTCGAAAGTCATCTGAATCAGATATCTTCTTTTCAATCAGTGCGTTCACTGATCGTCGGAAGCTTTAAAACCCAGAAACAAGGCGCCCAGTAATAATGATAAACACACAAATTCGTTTATGTAAAGAAATACTATAAAATTTGAAAAATATTTAATTTAGAATAATACAAAATATAAAACTTTTAGGTCTAATACAGATGTATACATTGAATTAATTAATTTTATACCATTCCAAATCGTCAGGTTTGTGGGAAGACGGCGGATTTACAATGGCCTGTGCGTCATTGAACAAGTTACTGCAAAGATTATCCTCAATCTGAAAGCGTAAAGTGGGCAAAGTGAGGTAGTGGCGTGGAACTCGGTTCGCCAACTCGGCGGTCGGCAACTGCGAATGCTTACCTGGAGGGAAGACACTAAGTTATCTGGGCAGTCGAGCACGCTAAGCCCAGATTCTGC

AAAGGCGAGGCATGGAGCGATAAGGAGTGTGGAGTGTTCGGCCTCTATCTCAAGATCAGTACTATACTATATAGTGAAAGCAGAGATGCCATGGGAATTACGTGATCGGCCCCCCGCCCACTGCGCACTCAATGTTATCTAGCGATATCGCTACGATATCTGTGTGTCTCGTTGTCTTACGCTAGTCGGGGTGGTGGAACGAGACTTACGCATTCTGGGATCATTCCATGAAGTCGTTCGATCAATGTGATTTATAAAGTAAAGATCTCCGGACTCGGTAACTGCCTGCTCCCAACCATCCGGCAGTGGACCCAAATTGTTAGCAATGGTCGATGTGGTAGTTTGTTTTGTAGTTTGCAAAACATCTATCGAACAAAAACAATGAATTACAAACCAAAGTAAATAAAAACAAATTAAAATATGTCTTCAACGTAATGCTGCAGTGTGTAAGCGGATGAGAACGGAGGGGCACTGGCGCCCAGCATGCAGCACATATCAAAATCAATTGGAGTATGCTCAAAGCAAATCCAATCGAGCTCCCACTTCCGAATGCCAAAATGATCAGACTCCAACCCAATCCCGAA

 

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