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CRIMIC CR00815

Gene CG9646

CG9646

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:17049539..17049546	53F2	FBti0199625	79297

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACTGGCTAACACACAGGCCGAATTG	GATGTCGATAGGTGCAATAGTCGTCG

Images

Construct Design Sequences

sgRNA sequence	ACAAGCATACACATCCTTAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGAGTCTCTGCGAATGCTT	CCTTAGGGGTTGCCCTTTGT	ATGCTTGTGTGTGTGCCCTG	CCCTGGCATCGTACACCTTT

 

Amplicon 5'

Amplicon 3'

GGGAGTCTCTGCGAATGCTTCTATTTGAGCCACTGGCCACTCCAGAGGGCCAGCTGACGCGATCAATGCTTCGAGAGCTGAAGGAGCTGCAGGATGATATAAAAAATCTTCGGGAATCCGATGACGATGTGGTAAATCAATAAGATCTGCGAGTTTGCAGATTAAAACTGGTATTTGAATTCAATAAGCATTGTTTCCCATGTTTAACTAATGACTTATTCATGTGTATAGCATTCATCTTGTTGATTAAAAATTTGAAAAAAAAAGAGGATACAATATTAACTGAGCTAAATTTCAAATCAAATCAAACCGAAATTGTTAGGAATGTTCACGAACAATAGGAAATAATACTAGTTTGCATTACTTTTTAATGAAACTTTAAATCAATTTAAATTCGATAACTGATATAGAGCTTTAATCGAAGAGCCGCACGTTTGCTGTTATCGTTTAATTTACCGCTTTTGCAACACTACTGAGTTTATTTATTGTTATTTATCTTTTTTTTGCATTAAACAATAAAAGCTCAGCTAGACAAAAGAGTTAACCGTGGAATTTTCGACATTTCCGAATAAATAACACAAAGAGGCAGTGAAATTATGGGAACAGCTGATAACTGATAATACAGATGGGGTATGCACCGCGAATAAGGGATAAAACAACCCCCAAGTGGGAAAAAAACGAGGGTTTGCTGCTCAGTTGCGATTTGCATGTGCCGTGGAGATAAGGTGCTCCTCTGCACCGCTTGATTCTGCGACTGCAAAAAATTTCCAACCGTGGAGCAACAACAAATGCTGCAATATATACATCACTAGCCGCGTCCCCGCAACGTTGCCGCCACCCCCGCAAAGGCCGTAATGTGTTGGACGTTGTGAAATAGTAATACTCACCATTCTACATCGACATTCGTCATCCGGAAGCGCGGAGCCGTGAAAATCCCCCTTTAGGATGGCCCTCGCCAGTGCCGCCGCCGCCCTCTTGAAGGGTTCGCAGACGCCTCTGCAGCAGCTCCTGGAGGACATTAACTTCCAGCGCACCAAGGAGATGCGTCAGCTGCTCAAGGATGGTGGGTACAGCTCTAATCCGGACTCCGCAGCTCCCCGATTCGGTTCAAATAGCAGGGAAAATTTGTCTACATTTGCACTTTATCGATTTATCACAAAGGGCAACCCCTAAGG

ATGCTTGTGTGTGTGCCCTGCTGCGCTTGTGTTTGTGTGTTCGTGCCTTTTCCGCCCCACTTTTGGCCAACCTCTTTGTTTTCCTTTACATAAGCGGCTTTTACTTTTATCGGCCCAAATGGCAGTATGGGAAAACCGCAAACAGCTGTTCACCTGTCGGTCAACATCAGCATCCCCCAGTTTCCCACAAGTCCTCAAAATCGAAAAGATAGAGCCGAAGCTAAGGTTCCTAGAAATATGATTGCCAAAACGAAATTCTCAAATTTGAATATACATAGATAGTTAATTATTTTTATGATGCAATTGAAGTTTGGGATTTAATACGTTCCATTTATAGAAGCTTAGCAACGCTTTTGGTCATAATAAATCTATATAACAAATTATGTAATACACTTAGGCATTTGATTTCATAACATTGACGCTGAAGCTCAGAAAGTTAATATTCTTATTATATGTCTCTTAGATGGCGGTTTCGTTGTCCTACAGGGCACCACCTACTGGACGGACCTGTTTGTGCGTCATTTCCTCTTCCAGACAGAGCCCGTGCACAGCATCGACTCAGACGACTTGCTCTTCTTTGTGCGCAAAAAGCACGTGAAGAGCTCCTCTCGTCACATGCCAAAATTTGAGGTGAGCATCAGGAATTACCATAAAACACCCATTGAACATATTCTATTCTTTAAACAGACTGAGGTGGAGGTATTCCGCAAGGACTCGCGCAAACTGCCCATCGGTGATCCTGATGTGGACTGGGAGGAGACGGTTTATCTCAACATGGTCATTCATCAGTTCGATTACACGTTGACACTGGCCATTTGTACAAGGACGTCGCCAAAGGAGTTGCAAGTGCTGAGAAGACATTCGCAGAGGGTCTACGCAAGCCCCAGTCGTCGGAAGATGGACACCAAGGGCGAAGGCGAGGAGATTACGTATCCGCACATCTGCTTCATGGTCGATAATTTCGACGAGGTGTTCAGCGACATTTTGGTGCGCGACGGGGAGATGGTGTGCGTCGAACTGGTGGCTAACGACAAAGATGGAGCAGTGCAAGGAGTGATCTTTTTGGGGTCCATACGCTATGATGCCCTAAAAAAGGTGTACGATGCCAGGG

 

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