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CRIMIC CR00816

Gene CG8399

CG8399

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16023900..16023900	52D14	FBti0199626	79298

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTACAATGGCTGCGGACAGAGCAA	CAACCTACAGGTAGGTGCTACTAAGC

Images

Construct Design Sequences

sgRNA sequence	ACTTCGGGGTCACATGAGAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTTCATGATTGCCGCATGG	CTTCGGGGTCACATGAGAGG	CATGTGACCCCGAAGTGTACA	AGTCGCTCAAGCTGTATCCG

 

Amplicon 5'

Amplicon 3'

CCTTCATGATTGCCGCATGGATTGGAACCACCTCGCTGGGCATCATCTTTGCGCGCTACTTCAAGCAGACGTGGGTGGGCAGCCAGAGCTGCGGCACGGATCAATGGTTCGCCTGGCATCGCCTCCTCATGGTCACCACCTGGTCGCTTACGGTGGCCGCTTACGTACTCATCTGGGTGGAGCTGAAGCAGGCTGTGTGGCATGCCCATTCGATAATCGGTCTGATCACGGTGATATTGTGCTTCATTCAACCCATTGGAGCTCTGTTCCGACCGGGACCCAATGACAAGAAGCGGCCATACTTCAACTGGGGTCATTGGCTGGGCGGTAACCTGGCCCATATTCTGGGAAGTGAGTTCAGCGGTCGTAGATCATCCAAATATATGGCTTATTCACGAATCTTCTTGCAGTTGTTACCATCTTCTTTTCTGTAAAACTACCAAAGGCCGAGCTGCCCGAGTGGATGGACTGGATCCTGGTCAGCTTCGTGGTGGTGCATGTACTCGTCCACCTGATATTCTCCGTAAGTAATTCATACTGCAGTGGTTGGGTGTTTCTCCCCTCTCATGTGACCCCGAAG

CATGTGACCCCGAAGTGTACAAAGATTAGACCCAGCATCGCCTGCCTGCCTGTACAGTGGTTCTAGTTGTGTCCCTAAACGTTCATTCATTCCATACTAATTCATCTGTCACCCATCATCCGCTCGAGAACTGGTGGCTATGTCCCTTAATGTGTAGATCGGCTTGTGTTTGAATCATTGGCAGATTGGCGGCATGGCCTCGGAGCGTCATCTCAGCCAGCGGGCGAACACTTTCCAAATGGGCGACATGTCCCATCATCAGCAGCACGCCATGCGGAATGGCATGAGCATGGAGCGGAAGATGGATGCCCCGGTAAGTAGTAGTAACCTATATTGTCCATATTCTTCATCCTGTAACTTCCCAATTTCAGTATGCGGGCATGCGCAAGGGATTGCTCGGAGTTTACGGCGTGGTGCTGATTCTCTTCGTGACGGTGCTGATCCTGTTGGTGGTGCTGGCGCCCATCGAACAATTCCTGGGAAAGTCCTAGGCCCCCTAATTCGGATGTCCTGTAACCTGTCCCCTTCCAATACTCGTGTCAGTTTTGTGTGCTCATTGTTTAACCCCTTCCGCCCACGGCTCTGCCCTGCCCACCACCCAAATTCCCATCCTTTTTCCATTCAGCTTTGTGTTGTTAAGTGTAATTAGGCGCATGTGTAACCTGGGGCGGAGCGTTCAGTACATGTTATATAATTTATTATTAATAAAATAAAACAAGCCAACATAAATGCAGTACTTTCTTTCTCTTCTTTGCTGCTATGCTCAATTTTAATCTGCTGCGCTACATGCATATATGTTTTACCGGTAGTATCTGGTGCTGGTGAGTGCTGATCGATCGGATTGCGTTGGATTATTCTATGTTTAGTATGGATTTGTATAAAACCTTGCAGCTAGAAATTATCATTATCTTTAGATATAGGTATATATGTATGTATGTGGTGTGTATGCTTTCGTTAAAGTGTTGCTGCTTGCTTAATTATAGTACGTAAATTATAGGTAGATCTACAATTTTCAGATATTAATGCAAAAACTAAACCCAATCGAACGAAACGGCCTTAAAGTAACAAGCGGAATCATCGCTCCTCCAGGATGACCGTTTCCTGCGGATACAGCTTGAGCGACT

 

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