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CRIMIC CR00818

Gene CG13096

CG13096

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:8510496..8510496	29D3	FBti0199627	79299

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAGACCAAGAAACTGGCCACAGACG	CATCATCATCGTCTTCCTCGTCATCG

Images

Construct Design Sequences

sgRNA sequence	CCTGCTAATGAGTGGGTTCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGGAGCTGCTTGATCACCA	GAGTGGGTTCCAGGAAGACA	ACCCACTCATTAGCAGGCAG	CTGTCTAAGCCTGCCAGTCC

 

Amplicon 5'

Amplicon 3'

CTGGAGCTGCTTGATCACCAGCAGAATGTTCTCAGCCAACTGCTCGGCGGTGATTTCGTGGTTGCCCACCGGAACGGCAATTAGATCTCCCTTTGACAACTGCCTGAATGCGGTGCGGGTGAGGGCGCGGGTCACCTCCTGTGGCAGCTTATCGTTGTCCTTGCTCAGACGCAGTGAGTGGAGCACGTTGCGGGGCTTCTGGGTGTTCTACAAATGGATAAGGCAGTGGTATTGCAAATTTAGAAAATAGTTAATAAATTCTATTAAAGTTCTTCCTAGATTTCTATAAAATCCGCGTAATTCATATGGCTGAATAAAAATACAGTCTCCCGATTTGAAAAATGTTTTACAATTTGGAAGAGAATAACATTTAATATGGCTGGATTAATCTCATTTCATCATAAAACATTGCGAAGGATACCCTGATTCAGAATATATATGAATACCTTAAAAAAATACATACGAATGTTCGCGACCTTCCCACAAGATCTATTTGCGGACAAAAGAAAGATTTGTCTTCCTGGAACCCACTC

ACCCACTCATTAGCAGGCAGAATTTCTTACCTTGCCCAAAAAGGCAGTAGCCTGTCCTGACAAACGACCGTCGCACAACAGGTAGTCGTAGCTGTTGAGGAACTTGCGCTTGGCCTCAAAGGAACCCATCTCGTTGCGCAGCTGGTTGAATGGAACGACGGTTAGGCGCTGTTTCACACCAGCCTCTCGCAACATATCCTCGTAGTGTTGCTTGGTGGGATCATAGTCAAACTTGGCTCCACGCTGCAAATCTGGCACGATGAGAGCCACATCGTCGTCCTTGCCCACCAACGAATGCTTCAGATTCAACTTGACCATGCGCTTTGGGCAGCTGGGGATCTTGTAGCTGCAAACTTGCAAAACATAGCGGTAATCAGAGAAGATCGAGGTGTTCTTCTTCTTTTCCACCTCCTCGGAGACAACAGACTTCAATGCCGCGCACACCTTCGTCACATTATTTTCATTGACAATCTCGTGGAACCGTTTCTCGTCAAAGGCCTTCAATTCGAAGGTCAGCTCAACTGGTTTTTTAGACTTGGCAGGCTCTTTGTTGACAGCGTTGCCTTTGGCCTGACCAGGCTTGGACTTGCTGAGGGCCTTCTTGGATTTGGGTGGACTGGCAGGCTTAGACAG

 

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