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CRIMIC CR00819

Gene rho-7

CG8972

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:11987936..11987943	48D5	FBti0200698	80676

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ACGTGCTTAATGGTGTTAAGGCTCGC	ATTCAGTAGACCAATGCGCTTTGCCC

Images

Construct Design Sequences

sgRNA sequence	TTGCGAAATTACGTACTTCTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCTGAGGTAGACCGCTAAG	ACGTACTTCTAGGCAGTATGC	GCAAGATCTGGGCGTTGTG	ATCCGGAGTAACCCCACGTA

 

Amplicon 5'

Amplicon 3'

CGCTGAGGTAGACCGCTAAGAATTGCTCTTTACCCAACGATACAGCCGCAGCGTTGGCAAAGCTGTGCATCACGTACATATTGGCGAAAAGGTGCATAGCCGAGTAATGGCTGAATGTGGACAGGAACATGGGCCAGCAGACGACTTCTGCGAGAAGGGTATCAAACACAATTAGTTAGGGGGGTTGTAAAAAACAAATATCATAAAATACTACAAAGTAAAAAATAGTTTATAAGTCCAAAACTTTTTGAACTTAACATTTGAACATTTGTTATTCAGGTAAAACCAAAAGTTTACATAAAAAAGATGGAAAATAACAAGCTGACATAACTAGAAGAAATTCGACCACTTTCTTATATAACCATAAATAACATGGATTTCCGGTCGACTACTATTCGTCTAATACTTTTCACGTGTAATGTATTTAATTATTTTTTTAGGCGATTTTAATAATTGAATGCATCTTATCTGATACATTGCGATAATGGCCAAAAAATGCATAACCAAGATAACTTTATACTTCTATTTTTTGTGATTAAAAAGGCAATCAAATCCATTTTGGCATACTGCCTAGAAGTACGT

GCAAGATCTGGGCGTTGTGATATCACTTTTTCGGTTTCATATTGGATTTTAGCACTTACTCGCCGCTGGATTGGATGTGAAGTAGGTAATCATTGTGGATTTCAGAGCGGGCACCCGCCACATGGCGAAGGCCACCAAATTGCAGAGTAAGATAGGAGCAAACATCTTGTCGCCGGGTGTCAGTGAGTCCCAGTGCCGCCGGATGTCTTGTTTGATCTGTGTCCAGTAATCCCTGTCCGCCAGCGAACGACTCTGCCACCAACCGAATCTCGCCTGGCGAGCCTTTTCTAGGATTAGGCTACGTGTGTTCTCGTACTCCAGGATGGTGGCACCCGCAAAGCAGCCGACCGTAAACTACAAGCGTGAATCAGGGACTATATGAGATCCCCGTACAAGTGCTGGTCATGATTAAATCTTACTGCTCCCGTGAAGGCCACCGCCTTGATCACATTGTTCACCGGCACCGGGGTGTTCTGCTCCGCTGCCGCAGGCTCCCCAGTCGTCGGCTTCAGGTTGCTGCTCTGTTTTCGGTTGCTGTGAATCTGCCGACATGACCTCGCCGCCGGATGACCAGAGTTTATCCGCAGGATTGGCACATTCACATTAGCATGACATCTGCGGCTGTAAGTTTACGTGGGGTTACTCCGGAT

 

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