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CRIMIC CR00820

Gene CG12325

CG12325

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:10826765..10826767	47C1	FBti0199628	79300

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTGGTTCTTGGGACAAGACCGTCAA	AGTGACAATACCCAGAGTCGAAAGGG

Images

Construct Design Sequences

sgRNA sequence	CCAAGGTAAGAGCTGGGGCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGGAGAGTGCATACTGGCG	AACCAAGGTAAGAGCTGGGG	ATAACCCCTTCCAATACCGC	AAGACAAGGCGCGTACATCT

 

Amplicon 5'

Amplicon 3'

ATGGAGAGTGCATACTGGCGGCTGGAAAGTCTGCAAACATATGCATTTACCATGTTAGGGAGGCAATACTTTTGAAGAAATTTGAGATCACACAAAACCACAGCTTAGATGGCCTTAATGTGAGTAATCAAAGAAATTTTGTACAAATATCCTTGTATTCACTGTATATTTTGTTCACAGGACTTTATCAGTCGAAAACATTTAAGTGAATTTGGCAACATGGCTCTTGTGGAAGAACGAGAAGAGCTGGAGGGCGGCAGGGTGGCTATTCGATTGCCGGGCGTACAACGGGGAGACATGTCATCCAGGAGATTCCAGCAAGAGGTTAGGGTGTTCTCCGTAAAGTTCTCACCCACAGGCCAGGCATTTGCGGCTGCTGGCACTGAAGGACTATGCATATACGCCTTAGATAAGGGTAGGCTCTACTAGTATTTATTATGATTACCATATATATATTGACTCTACGCATTTCTGTTCAGGTGTTGTCTTCGATCCCTTTGATCTCTCGCTGGAAGTTACGCCGAAAGCGGTCCACGAGTCCCTCAAGCAGCAGAATTACACCAAGGCGTTGGTCATGTCCCTCAAGCTAAACGAGCCCAATTTGATTGCCCTAGTGCTGGAGCGAGTGCCGTATAAGGACGGTGAGTAAAATGCTAAATCGCCTGCTGCATTCAGGGGCCAGTCCTTGTCAGTTTTGGCAACCTTCATCGCTGCCGCGACTAAAAATAGCGGCGACACACTCCGCCGTAGTGGCGGAGTGGTTCGCCACTTGCCCTGACTCCGTTCTCCGATCGAATGTGCAAATTTGCACTTTTGCTGTGTTAATAAACAGTTTCAAGGTCCTCGCCCCAGCTCTTACCTTGGTT

ATAACCCCTTCCAATACCGCTTGCAAACGAACTATAATGGTAGTACATGCAAATGGCTTGCCGTAAACAGCTTTGGCAGCACGTGTCTGTATTGCATGCATATTGAGTCCTTGCTCCTTTCGCTTTTCCAGTCGAACTGGTTTGTGCGGATCTGTCGCCGGAGTTTGCCCAGCGACTGTTGCAACAATTGGCCCGTCAGCTCCAGTCCACGCCACACATAGAGTTCTATCTGCAATGGAGCTGCTGTCTGCTGACCAAACACGGAAACCAAGATGGAGTCTTTCAGCACACGGGCTTGCTGGCACTGCACGAGGTTCTTTCGAGGAAATACGAAATGCTTAACAAAATGTAAGTATACTTTTTTATATTGTGTAAAAGGAGATTTCACTAAGTTCTTTATATATGCAGCTGCGACTACAACAAGTACACGTTGAAAGTACTCCTGGATAGAGCTGATAAGTTGGAGCAGAAAAACGAGGGCAAACCCAATGCGACGGAGGAAGATAGTGATGAGGAAATGCTACTTATCAGGTCGAAGGACGACGAAAATGGACAGGCGCAGTTTAGCGATGAGGAGGAGAGCGACAGCGGATCCGAAGAAGAGATGGCTTGAATCGGAGCATTGCCAACTATTTCCTTGTTATCTAAATCGACGCATACTTTGATTTGATAGATGTACGCGCCTTGTCTT

 

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