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CRIMIC CR00821

Gene Socs36E

CG15154

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:18141324..18141329	36E6	FBti0199629	79301

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCACCTTAGCAAGTTCTCAGCACG	GTCCTTGTAGTGCTCCAAAAGTCCTG

Images

Construct Design Sequences

sgRNA sequence	GGTCGGATTCTCTTCTATATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTGAACTTGTGACCGCTCT	AGAGAATCCGACCTAATCTCATC	TATTGGTCAGCTGTGCACAG	GGCGTGGTGACAAATCGAAC

 

Amplicon 5'

Amplicon 3'

CCTGAACTTGTGACCGCTCTGCTCGATGCGAGCGTGCAGTGACCTGCCGTACTTCCGGAATGTGACCGAGAAGAGGAACTCCTCCTGGGCGGAGTCGCGCAGCAGGAACGTGCCCTCCGGCTTGCCCTCGAGCAGGTGCTCCGCCTCGTAGCGGTCCATCTTGCCCCAGTAGAAGCTGCTGTTCGTGATCTTCTCGAGATCGGGAACCAGGCAGTGCATGAAGTCGATTTGCGAGTGCACGGTCATGTTGCGGGCGGCTCCGATTCCGGCATGGCCAGCCCCTCCGGCGGGGGCGACCGTGGCCAGGGGACGTGGCCGGGGCGGAGTCTGGGGCGACTGGGGGGCGGGCTCGGTGACCACAGTTACGCGGCCGCCATTGGGCGACTCGACCACGAAGCCCGGCGATGTGATCCGAATGATGAGCTGATGATGGTGATGGCTGGCGTCCGGAACGGCGACGGAATTTCGTATCCTTTGGCTCTGCATCCGGCGCTGTGAGCCACCACTCGATGTGCCCAGCGTGGGCGGTGTGGAGCGGCGGTCAGCTGTCCGGGATTTCCTCGGAACCATCGCCACCACCGCAGCGGCATCCTTTTTATTGCTCACGGAACTGCTGCTGCTCGGCAGCATATCCCTTAATCCTCGGATGGCGTCGCGGCACTTGCACTTGCAGCATCGTCGCTTCGAGGATGTGGATGTGGACACGGATGTGGATGCGGATCCGGAGGCGGAGGATGTTGCTGCTCCCATTGAAAGTGCTTTACTGCTGCGACTGCGGCAGCAACTGTTGCTATTATTGCTTCTGGAAACTGTTTTTTCGGAGAACAAAATTTATTATTAATTTGATTCACTTTAAATTTGATGAGATTAGGTCGGATTCTCT

TATTGGTCAGCTGTGCACAGCGAAAAATCCCTTGATCATACTATACATTTCGTCGAATTCATTGAACTGAACAGAAAGTGGCATTTCCAACGTTTCACTTTCATGAAATTTTTTGGTGAGTAATTCGAAATTATTATGGTTCTGTTCTGAATTCTAGGCAGTAGTTAGATTGTTTTGATTAGCTTTTACTCACTTTAAAAAGTTCATGATGTCACTACTTGATTGGATTTCATCACTTGGTTAAATTTTTGAAATCACACAACTGAATCTTAATTGCTGACAAATTGGATTTAAATATTATTCACTTATTATATGTTTTGATTCGTTAAAAAAAAAAATCGTGCCGATTTTTCGCTGTGCACTTTCCAAATTGAGAAGAGAGTTCGCTCGCTCTCACACTCCGACTTTTGCCCACCTGGCTTGCTGCTGTGTTGCGTTCCCGCCGACAACATATTGCTGACTGGCAGCGACTCGAGCACTTCGCAATACTCGACGACTTCGGCCTCGTGCTGCTGCTGCTGCTGCTGCTGGTGGTGGTGGTGGTGGTGGTGGATGTTGCTGCGGCTGTTGCTGTTGCTGCGACGCCGACGCAACAGCGACTCATCCAGGTCGTCATCGTCGTCGGCTTCCAAGGGGCAGTTGGCGTTGCCGCCATGGTTGTCGTGCAGTTGTAACGTACTTATCGCAGCGCCGACGCTTCCCGCTAAACCGTTGTTATTCACGGGCTGTCGTTCGGTTTTGGCCGCTTCCGGCGAACTGCCAGCAGTTCGATTTGTCACCACGCC

 

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