CR00823

CRIMIC CR00823

Gene Asph

CG8421

Information

Donor Location Cytology FBti Stock number
pM37-p12R:16111434..1611143452E5FBti020069980677

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TGAAATAGCGGTGCCTTTACTGCCCA CCTCATCCTCATCCTCATCATCTTCC

Images

Construct Design Sequences

sgRNA sequence ATAAGACGGCGCCGGTTGGGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAAACGCGGTAAGTCCCTGT AAGCTGATAAGACGGCGCC GCGCCGTCTTATCAGCTTGT GCACATCGTGACCATCATGC
 
Amplicon 5' Amplicon 3'
AAAACGCGGTAAGTCCCTGTTACGGTACTTTTACTTACAATCATACTCGAATGGACCGTTATTTGGTGTGAAAAACGGTTTTGTCTTTTCGCCGCCGCCGCGTAGCAAGCAGTTCATTTCTTTTATTAGCATAATAATATTGTCGAGTGCCTTTAAAAATAACTTTGCCCTATTATATAAAATTATACATCTCGCCTGTTTTCTGTCGCTATGCGTGTGCCGATCGAGCAGGAAAATATATATGCATGACATGTGTGCGGGCGAGGAAGGCGGGAGGTAGACCATAAAGTGGGTATTTCGATCGGTAGCCAAAGAAAAAGGGCATTGAATCGGTGTGCAAAAATCGAGAAAAATCAGAGAGAGTGGTTTTCCCTGATTTATTTATCTTTTTCCCAAGGCAAACAGATGTTTCGTTGTCCCTGGGGTATCCTCTTATTGCTATCTCTCTCTGCCACTTTTTCTCTCTATTGCTCTCTTCTCTGCGTTATCTTTATCTCATTGGAGAATGGAGAGCTCCGTCACCTTCTCGGCAAATTAATTCCAATGCAACAGAGATCGAGATTTACATACACAGATACTACAATAGATACCAGATAATGATGCTTCCGTAAAGATTCTGGGGCCATTACACCCACGGCACCGAAATAAGTAGCCACGATGGTGCATATATGTGTAAAGCCATTTAATTTCACTTGGCCAACTGCCCAAGAGCATGACTGGGCGGCTTCTTTGTTTATCGTTGCGCATATTTAGGCATAAACAATTCCCCCCAACCGGCGCCGTCTTATCAGCTT GCGCCGTCTTATCAGCTTGTAATGCCGGTATATGTCTCATTGTAACTAACTTTTCGTCTAGATGATCGCAAGTCCGAGGGCGATGTGACTGTTCTGCGTCAGTCCAGTTTTCAAGGTAGAGAAATAGATACCCCCCTATATGTATTTTTCCCAACACACCCCGGAATGCTTTGCCAAATTAATTGAATGTCTCGCTCAGTTACTAAAACCAAGTAAAACACTCGAAGGTGGATTCAATTAAACTGCTTGTAATCACATGTGTTTTTTTATTATTCACTATCATGCAAATGTCGGATCTCAGATGACGACGAATCTTCGCACGGCGTTCACATTACAAAGATGGGAAACGAAGATCTCCACCTGCATGTGCACCACGATCACGGAACTGGCGGTCACTGGTGCGCCAAGATTATATTCTTCGCCCTGATGGCAGTGCTCCTCGGTTTGGTGGGTCTGATCATCATGGAGAATCGCGGACTGGAGGATCGTAAGTTATAGAATTCTTAGAAATTTGAATTGTACTGAATACCTGTTTTATTTTAGTGGACACTCCTCTGTCGGAGTCTAGGTTCTCGAAAGTTTTTGATGGCTGGGTGGACGAACATCGAGATGAGCATGATGGTCACGATGTGC
 
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