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CRIMIC CR00826

Gene Kua

CG10723

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:20101527..20101535	38C1	FBti0199631	79303

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AAAGCAGCAAGAGAGCGACATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCCATCGCGTTTATCAACC	GCGACATGGAAATATGCAAACT	CTGCTTTACCTCAGCAATGGG	GGCCACGAATATGGAGCAGA

 

Amplicon 5'

Amplicon 3'

GCCCATCGCGTTTATCAACCTACCCAAAAAAAAAAGGAGCTAGATAGCCTTCACAACAATTTAAATACCAATTTGCCTAATTAAGGCAACTCTAGACCGCGCCTCCGCCCGTGACAACTCAACTCAACTTAGCTTCTGGTGGTCCCCGCAGACCAGGCGTAAGTCCAAAATATTGCGTGACCAGTCGTTTGGGTGCTAAAAGGGGTGGGGATTCGGTGCAGCTGGAGGTAATGACATCAACGAACGTCGCAACATGCTGGGATCCCTGACCAGAGGTCATCGGTTGGAGCTACGCGACGGTGTGTCCCTTGGTTGCGCTCCAGCCAAGGCGGTTTGGTTTCGAAAACATTTTAATGATTTAAATATATAGATATACAATATATATTTGATGCCCACGTGGCTGGCCTCGCAGTCATCAATTATGTAGGGGCGACGAATTGATCCAGATGCCATTATTCACAGGCAATCAAATCCTGTGGCCTGACATTGAATTCAGCCATTGCATTGAATTCAATCAGTCAGGAACGGTCATCAAGGCCTCCCAGTTGTTTTCTCCGCTCGACTCCTGTTCTAAACTCCTTCATCCGCAGACGATGGCGGCCAAGAGCGATATGGATATTCTGGCCAACTCCATGTACGAGGACGATCCCAATGGCAACCTAGCGCCCGCCACCACCAAGGATAAGGAATCAGTCAAGCCGGAGGAGCTGAAGGTCACCCCCGCTGGCACCAAAGCAACATCCCCCCCCAACTCGGCGACCTTAGTTTCGACTTCTCCGCGATGGGGTCCGCAGAATAAGGGCGCCCAGGAACTAGCCTTGTTGTACAGTCCAGGTGAGCAGTTCCCACGGATTATAAGCTGTTATGAGAAAGTTACCAGAAGGTGAACTAGAAAGTTTCTTGTCCGATAATTACAAAAGAAGCAAACTGCTTTAAGATAATGTCCGATTATATATCAATCAAATACTTGCCTTGAATGTCCTATACAACATATATGTATGTACATAAAGAGCGCACTTGTATATTATTACTTGTATATCTCACGTCTTTTGCCTAAGCAGTGGGTATACGATAGCGAGGCAATCATATATATTGGCTTCTGCAAAAAGTATACAACAGTCTGTACGTTAAGAGTGCAATCTCTGAAATCTCAGTTTGCATATTTCCATGTCGC

CTGCTTTACCTCAGCAATGGGTATATGACAGTCGAGACACTAGATTACATTAGAAAATCCTGGGGTTTTATTTTATTTAAAATTTTATTTAAACAGGAAAACGTGCCCAGGAAATCATTTGCGTCTACACCTGCATTGGCCTTATGATCATTAACCTAGCCCTGATTGTTAGACACTTGCGATTGGAGCGGATTAGTGTCGCCTTTCTGTCCGCCCTGTGCGGCATTATAACGGCCGACTTTGCTTCCGGCTTGGTTCACTGGGCGGCAGACACCTGGGGATCGGTGGACATACCGATGATTGGAAAGGTAAGCCAAATGTACATCGAGAATCTAATGTGATAAATGATACAAGACATATATTTTGAACTCTTCTAGAACTTCCTGCGACCCTTTCGCGAGCATCATCTGGATCCCACATCCATAACGCGACACGATTTCATTGAGACAAATGGCGACAACTTTATGGTTGGCATACCAATTCTCGGCTACTTGGCCCACTATTTTTACATCAGGACGCCCAGCGAGATACAGCAACACTTCGGCTGGATAGCCTATGTGTTCCTCTGCTCCATATTCGTGGCC

 

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