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CRIMIC CR00827

Gene mRpL13

CG10603

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:18858568..18858568	37B1	FBti0199632	79304

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAAGACGAGGGAGACAACAAATCCG	GTAGTTTGTGTGAAGTGCACAAGCCG

Images

Construct Design Sequences

sgRNA sequence	GCGCAACTGAATGACGGTTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCAGGGTTGTCGAGGATC	GCGCAACTGAATGACGGTTC	GTCATTCAGTTGCGCTCCAA	CACTGTCTGCCAGCTGAGAA

 

Amplicon 5'

Amplicon 3'

TTGCAGGGTTGTCGAGGATCGCAAAGTGTTAGACACAACAATCTATTAATTATGAAATAAATTAAGCAGCTTGCCAAACTAAATACGTTATTTCCAGTGCCAAGTTACTTTCTTTAAATTCTTGCTATTCTGACATAAATTGTAAAGTCTATAAAAATTAACCTGAAGCTTAGCTGCCTGGCATCCCTTTTGCAGTATTGCCAGCCCAAAAAAATCTCACGCTTTTAGATTAGTGAAGTCGCCGCCGTTATACCATGTAATAAACAATAAAATCACATAAAATTTGGCTATAGAAAATAAGAATTGAAAAAAAAAAATTAAAACTTCAATAAGCTTTTTCCATATTTTGTATTTATCTTAATTACAATGATTTCCTATCAGCATATCAACGCAAGATATAATCCTTGGGATAATCCATAATGTTGGGGAAGTTCTCCAGCGTTTCTTTGTCGATATGATCCAGACGCTGGGGAATAGAGCGCGGCGTGCGGATTTGATTCGTGACGTTTTGCAGGATTTCCTCGGGCACCTGATCGTCGGCGAACAAGTGCAGTCTTTGCATGGTGTGCCTGCGCTGCAGATTTCCGCGCATCGAGTTGTACACGGCCTTCTTCATCACCATCGTGGGATCCTTCTCGTGCAGCTGCCACGCCAGGGTCCACGAAGCGCCACCAGGATAGCCGGTGTGGTGGAAGTAAACCCTCTTCACCCACTCGTCACCGGGCAAAGCGATTTCCCGCGTGTTGATCAGCACCACATGATCCCCGCAGTCATCTGGAAAATTACATGTACGGTTTTTATTGGATGGCCGTTGGGGATTACTTGCTTAACCTAAAAGTACAATAATCACTGGTCAAAAATAAGGTATGAAATACCTGAACCGTCATTCAGTTGCGC

GTCATTCAGTTGCGCTCCAAATAGTAGTTTTAGTCTTAGCAGAGGAAGTAACTATATATAATCGGCGACCATTTAGTAGATCCTTATGAGTATGAGGATTTTTAATTAAACATATTTGTTTAAGGATTAAACAAACAAGGATTATTACCGAATTCATACTACTATTTGAATCGCAGGCTGAATGGTCAAATAATTACCGTTTTTGGATTAAAATTTATTTGTTTTCATTTTATACTTGCAGCAGCAACAACAACAACAACAACAACAACTAAGAAAACACGCCGGTGATGTAATGACATCGAAATACCCCAAAAAAGGGCTTAAATCAAACGCTGTCGAGATCGAAACTAAACAAAAACTGCGCCAAAGGATAACACTTACTCATTGGGTGGTAAATGGGCTTTTGGAGGCCCAATAAATGCGTTTTAACCAGTTTTGCCGACTCGAATGGATTCTGCCAGGTGCAATCGTAAATGTGCCAAGTGCGCGCGAACGTTGCCCATTGCTAAATCCGGAGGAAATTTAATGATTACCTATTGCACATTGAGGGTAGTGAGCTTACGCACCTGCACACGCTTCGCAATGGACATTTTTTGAGATTTTTAATTAAACAATTTAAATAAAAATAATTTCTCAGCTGGCAGACAGTG

 

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