CR00828
CRIMIC CR00828
CG10413
Information
PCR Verification
PCR check relative to sgRNA sequence
HA-L |
HA-R |
Forward |
Reverse |
OK |
OK |
GTTATCCGACCCCAGATAGCGCATTA |
GGCATTCAGCCCTGTATAGTCGAGAA |
Images
Construct Design Sequences
sgRNA sequence |
GGATTCGTTACGCTCTGCTTTGG |
sgRNA2 sequence |
|
Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
ACGTGTGTCCAATTCCACCA |
CGGATTCGTTACGCTCTGCT |
GAGCGTAACGAATCCGCCTA |
GGCGACGGATATGTGGAGTT |
Amplicon 5' |
Amplicon 3' |
ACGTGTGTCCAATTCCACCAAAGTAAACCCTGACCAAATATTTATAAAAAGCCCGATTTGATCCCAAGATAGACTAGATATCAGTATCACATTTAAATTGATAAGACCAACAGGGCTTTTCCTCAATGAGCAATAGCTACACAGCAAAAAAGGTAATTTCATACTATATCACATAATCATTTAAAGTTTGTTAAAATATGTAAGGTCTATGAAAAATGTAGCAGTAGTAGCCCTAATTTTTTGTATACTATTCAATTTTTTAAATACAATATTTTTAAAGAGTGCAACAAAATCTTATCAATACATGGAAACATAAGACTTTTCCCCGATGTACTAAAAGATATCTTACCGTGTTATTGGTGGCGCCCACAAATAGAAAACTAAAGTAAGTGGCGAACAGGCAAACGGTGACGGTGGCCAAAATAATCACTGACGTCTTGGCGAACAGAGCGGCGCCCACCAGGCAAACCAGCAGTTGCAGGGTGTTTATCATGCTGCTGGTAAGGAAACGCCACCAATCCCCATCCGGTAGGTGGGAGTCCCCGCTCACAAAATGGCCACGCGGTCCGAAGTTATCCATGATGCCCTCAGTGCAGCCGGATATGGCCATTGCAGATCCCACCACATTGGCAAAGAAAAAGAGGGTTCCGATCGAGCCACCAAACTCCAGTCCCAGCGTTCGACTAATCATGACTGCAAAAAAGTGTGATAAAATAAATGATTTAATGAATGTAAACGAGAAAAACCAAAGCAGAGCGTAACGAATCCG |
GAGCGTAACGAATCCGCCTATTTTCGAAGTATTTCATAGTCAGAGATACTAAATAAGTAATATCGATTACCATATAAAATATTTGACAAGTCGTTGGCTTAAAAACATGCAGTTTTGCATGCATTTTTATTGTTTGTAAGACATACAAAATATATTAAAATGTAACTTTGTGGTTACTTTCAATAAATGTACATATGTATATACAAATGTATATAGATTAGAAAGTAATTTAATTATTGAAAACAAAGTAACAGATTAAATAATAAAATATGTATATATGAGAATTAAAAAGTATTTACGCGCTAATTAGTTAGTTACTCACAATAAACTCCCCCTCCCTCGATGGCTCCATTGGTGGAAATGGCGCAGACCGAAGCCACCGTGAAGAGCAGAATTCCGTAGGCGATGAGGAACTGCAGCAGGGTTACATACAGGCCCGCATTTCCGACGATGTACCCTGAACAAAGAGAGTTAAATAGTGGAAAAGACCGAGATCAACGGACCATCACAACAAGTGTTTTGTGACGTCATCGCCGCGCACTTACCGACTCGAATGAAGACCAAAGCACTAAACATGGACAAAGCCACTGGACTAAAAACTCCGGCAAAGGTACCGAGCGTACGGCCACTACGATCCGGGTCCTGCATTCCGAACTCCACATATCCGTCGCC |
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