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CRIMIC CR00829

Gene CG34174

CG34174

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:2214891..2214908	22D4	FBti0199634	79306

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCGACACGCCCAGTTTATGTATGTA	AAGGAATTGGACAGTGCTTTCCTGGG

Images

Construct Design Sequences

sgRNA sequence	AGGGGCTGCTTTCGACAAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTCAAGTGGCGTCCAGGTT	TTCCTACCGGCAACTTCCAT	TGTCGAAAGCAGCCCCTTTA	GTGGGCGACAAACGATATGC

 

Amplicon 5'

Amplicon 3'

ATTCAAGTGGCGTCCAGGTTAACATATTTTTGAATTTGGCTTTAGCAGTGATCTGATTGAAAATTCACAGGAAAATGAAGGATTTCTACACACAATTACCGGCACTTTTGACGAGCTTTACTTTTTTCTGAATGTGGTGTGTGAACATTGATTTGGTCGAAACATATCGATTATAAGCTTTTCAATTGTGTGTATTAAAGCAATTGATTCATGTAATCGATTAAGATTAAATGAAATAAATGTCGAAATGTCCATTCCCCATTTAAATAGCTATAGTTCTATGCTAACATTGAATATATTCACTTATCGATATTAGAAACCATTAGTGTTGATAAACGCGTTGAACGTATTTCCCAAAGAGGAGGTGTGCATACACTAGCTGAAGTAAACAATATTGTTTACAAAATGACCAAAGCGAATAAAACGCCGCAAAAAGCAAGTGAACCGAATATAAATGGTTCCTTCACACCAGTTCGATGTAAGTTGGCTCCAAAGACAAAAAAGGTTATTATTTCTTTGCATCGTTTCCAGATTTGGACAGCCAGCGCCTGCAATCGCCCACGAGTCACGATGCGAATTTAGCCACCTGCAAAACCAGATTGGAAACAATAGTCAAACAACTCCAGGATAACTACGCTAAATGGCAGCTGGCCCATCAGCGGGGCACCTCTATTTGCTATACTATCGAAGCCAAGAAGACCAAGTGTCTGGAGAAAAGCCAGGATGAAGGCAGCTCCCTTTACCCAGATGATCTCCTACTTCCCTGCAACAAGTTGGCCATCATAGCCTCTATTTTCGGGGATATCGCAAATAATACAAAGGAGATACTAAGACAACTGAGAGGCATATTAAAATTACCGGGATCTGCAGCGGATACGATATTCTACAGGTCCTGGAAGTTGCAGCAATTCGTGGTTTTCGCGAAGGAGCTATCCGAAAGATATGAGAAGGAGGCTCTGGTCAAAATGGAAGTTGCCGGTAGGAA

TGTCGAAAGCAGCCCCTTTAATACGTTTGAAAATTCAAAGAAGCACAGATTAATTTCTCAAATTCGATTTACCATCTTAAATATAATCATGTTACTTTTCTTATAGGTAACATTGCTCACTCGACTGAAAGAAGCCAGCTCATTGCGCACACAACTTTATGGGAATTTCCCGAGCACGTGGACTCCTATGTCCATCTGGGGTTTCTTCTCCTTGCCGAAGAAGTCAGTTTAAGGCAATAAATTAAACAATTTTTCAACTTAATACAACATATGGTTTCTATCAAATGTTCATGTTGGGCAGTAAAACTTCATTACAGCTTTAGATTAAGCGAATGCTTAAAGAAGTAATAAGTAAATTCTATAAACTTAGGTCCTATGAGAAAATCGGAATGTAACTATTAAATGCATCGTTGTGCGTTTTTTTTAAGTCTGAACGGAGATCCGCTCCAGTCGGGGAGCGCTGCGGAACTTGGTCCACCACCAGAACCAGGCGCACGAAAATCCCACAGCCAAGGTGGTGTAATACAAAATAGTAGCCAGGCTCCACTTCTTCTGCAATGCAAATTTGTATAAAGGAAAATAATTTCTTATTGCTTGCTATATTCACCTTCTTGTGACTCTCGTTGCCCTGCACTTTCTTCTGGCTCCTTGCGTTCTGGGCATTAGCTTCGATTTTCGCGAGATCCTTGGCAATCTCTTCGGTGATCTGATTCACGCTTTTTAGAGTTTCCTGGGCAAAGCAGCTGGCATATCGTTTGTCGCCCAC

 

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