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CRIMIC CR00832

Gene CG10338

CG10338

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:18696293..18696305	37A1	FBti0199635	79307

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACGCAGTAATAAGGCCAACTCCTCC	GGATCGAAGGAGGACACTATGTTCTC

Images

Construct Design Sequences

sgRNA sequence	CTATAGGCACTAAGAAACTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATTTCACGCAACGCTGACA	TCTTAGTGCCTATAGCTGCTGC	CACCATTGGTACTGTTCAAGCT	GAAAAACTCCTCCAGGGCGA

 

Amplicon 5'

Amplicon 3'

CATTTCACGCAACGCTGACAAACTGTAATTTTGCAGCGTTTAAATCAAATATTTAGTATGACCGAATCCGGCTTGATGCAAATAATTCACCACCTCCAGCATTCACCGCGAAACGCACCCACTGCACAGCTGACTGATAACACAATCGGAAGGGGAGAACTTCATTTTGGGGTATATTTATTTTGGGCCACAATTAGTTGCTAGCATTTGATTGAAGTAGCTCCAGCTCCATTGGCTTGTTTGTAAAATGCCCTAGAGTTCGCTTTCCAACGCCGCGTTCATTGATTCGTTCGAGTTCAGTTGCGCTAGTCGCTAGTTCGATGGCTTTACGCGGAATCCCCGCCTGAGATAGACAGCCAATCTAACTAACTAAGAAGCGCTGGTTGTGGGTGCTAGTGGAGCCAGGTGGGTGGTGCTTTACTGCTGGAGTCTCTGAAAGCCAGACAACCCAGCCAGTCCCGGATAGGCCATAAGTCATAATTTCCAGACGGCGCACAGCTACCAAAGAGCCAGGCACGCACAGCAAAGATGAAAATCCATTTTCGGGAACAGTACGGAAGAAAGGGGGAGGAAATCCTCTATGTGACGCCAGCAGGTGAGCAAATCGATCAAACCAACAAGCCAAAAACCTTGCAGGCGAAAATTCAGTAGCCATACAGCAGCAGCTATAGGCACTAAGA

CACCATTGGTACTGTTCAAGCTTCAATTTCTATTAATTATTAATCTTGTTTATTATTATTATTATTTTTGTTTGGAATTATGGCGTTTTACTTGCAATACCCTATATTTTTTATAGCATATCGACTTTTCAGACAAAATTTTGTTCAATGCCATCGTTCTCTTTGCTTTGTTATTTATTTATTTAGGTAAATGTGTATAAATATATTCTGAAACGATTAGGTTGGCGGTTAATTCATTAATTAATTTGTATTTCCAACTCCAGATCAGAGCAACATTGTGCGTGTGCCGCTGCGTGGGGATAAGCTGGTCATCCAGGAGAAGTTCCTGCTGTTTCGCGTGCTGCAGAAGGTCTTCCTGCCCAAGGGCTATCCGGATAGTGTCAGCGAGGACTATGCAGCATACCAGATCTGGGACACGGCCCAGGCCTTCTGCAGCACCATCTGCGGCACCCTGTGCACACACGCCATCTTGAAGGGCATCGGCGTGGGCAGCGAGAACATCAATGCCTTTTCCGCAACGGCGACTTGGATCTTGAAGGAGGGCAGTGGTCACGTGGGTCGCATTGTGTTCGCATGGTGGCAGGGCTCCCAATTGGATGTGGACTCAAAGAAATGGCGCCTGCGTGCGGATTTTCTGAACGATCTGGCCATGGGCATCGAGATCTACGTGCTTCCCAAGTATCCGCATTTCAGCACCCAAATCCTGTGCTGCTCGACCCTGCTGAAGGCGATTGTGGGCGTTGCCGGAGGTGCCACTAGATCGGCATTGACCCAGCATCATGCCTTGAGGGGCAACCTGGCCGATGTGGCATCCAAGGACAGTTCACAGGAGACGTGCGTGAATCTGGTGGCCTCCTTTGTGGGCCTGTATCTCCTCTCGCTGATCAAGAGTCAGGCGGTCCTGTATACCATATTCTACGTGGTGGTCAGCCTGCATCTGTATGCCAATCTGAAAGCAGTACGCGCAGTATGCTTGCGAAGCTTCAACGAGTCGCGCTATTTGATCGCCCTGGAGGAGTTTTTC

 

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