• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00835

Gene CG6686

CG6686

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:11987626..11987639	33B5	FBti0199638	79310

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCAGCTTGATCTTCACACACAAAGG	TTACATACCTTACGCATCGCCTCCTC

Images

Construct Design Sequences

sgRNA sequence	CAAGGGCGATTGCCGTAGGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCCTTTCACGTTCTCTGCC	GATCCGGCGGAAAAGCGATA	CAATCGCCCTTGAGCCTGG	CCTTTAGGCCGTTGAGTCGT

 

Amplicon 5'

Amplicon 3'

TCCCTTTCACGTTCTCTGCCAGATCGCTTGCGGGCGTCCCGTTCCCGTTCGATCTCACGCTCTCGCCTAGACTCATATTCGGCATGCTTGGAGATTTGGGGTGGTGGAGGAGCTGTGAGCTCCCGTACACGTTCGCGTTCCCGCTCCCTCTCGCGCTCCCTAAGGCGTTCTTTCTCGCGTTCTCTGATACGTTCCTTCTCACGCTCTCTCTCGCGCTCCTCCCGCTCCCTGAGACGCTCCTTCTCACGCTCCCGTTCGCGCTCCTTTTCCCGATCCCGATCCCTCTCCCGCTCACGATCTCGTTCTCGCTCCCTTTCTCTTAGGAGTTCACGATCGCGTCCAGAATCCCGCTCCTTGTCACGATTGGACGATTCTCTGGCGTCCCTCAACTTGGCCTGCGCCTGCACCTGCGCCTGTACCGGCACCTCCACGCAGTCGGAACCTGGAAATAGGGAACACCGGTTAGAACTGTCTAATCATCACAGCTGGCGAATCGTTTCAACCACATCTCCGCTTTCCCACTCACAATCTGCATCAGCAGCGGGATACCTGAAAAAAAGGAGAAACATGGAGAAACGCAACGGTGGTTTTAGTACGTTCGGAAATGGTAAAGTGCAGCACCAAGACAATTGCCGGGCAATGGGCGGTCCGTCAATCAGAATATCGCTTTTCCGCCGGATC

CAATCGCCCTTGAGCCTGGCCTGCACTTTGTGTGTGAAATATAGGGCAAACACTTACTATCCGAGTCCGATTCCTCTAGGGAGATGACCTCGTTCGCGCGCTCGCGCTCCTTTTGCTTGTGGTGCTTGTGGCGGTGCTCCTTGTGCTTCTTGTGCTTGTGGTGTCTGTGCCGCGACGAGGAGTCGTCGGTGAGATCCAGTGTGACATCCTCGTCCTCTGTGACGCGCCGCGGTCGCTCCGACGATCGCGAGCGATGACGGTTCTTCTTGTCCTTCTTGTGTTTCTTTCCCGACGACGAGGAACCCATATTTATTATTATTTTCACAACAAAATACAGAAAATTTCACAAGAAAAAGACGCCGGTGTGACCGCAGTTCGGGAAGCGGAAATATACCAGGTGTGCCACGGTAAAATTAAATGCCAATCACAGTGATGCGCGCACAAAGCCACCGAAATTGTTTAAGATATTAAAACTGAATGTAATTATTAAATTATCACGCAATGTAGTACTTCGGAACTTTTTATTTGTTAAAAGGACCTTTTTATTTAGAATTCGTTTTGTTAAAATTACCTTTTTGAATGACTAAACTTACACCGCCTACTCTTCTTCTTATTTTTTGTTTTGCCATTCACCACGAACTTAATAACAACTTAACACGCGGAGATCTCCACATAAACAATGGAAATTAAAGTACGTAAACAAATTATTTGCGACCTAAAACTTATTTCATTTTGATCCCCGTAGAAGTCCAAGAAATCAAAGAACGACAAGAAGTCCAAGGCGCCCAAGGAGTCGTCCGTTTCGCTCAAACTGAACGCTTTGCATCGCAAGCAAAAGGAAGTCGCCCGCGTGCTCACTTTAAAACAAGAAATATTACTGAAATCGGGTGTATCCTATCTGGAATATTATGAGATTCTGGCAGAAATCGAACGACTCAACGGCCTAAAGG

 

---

Search Again