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CRIMIC CR00836

Gene Thor

CG8846

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:3479113..3479134	23F3	FBti0200701	83196

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AATTCTGCGGAACACCTCTTGACTCC	CCACTATTTGCACTATCTCGTGCGGT

Images

Construct Design Sequences

sgRNA sequence	TGGTCTGCGTTGCATCAGAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAACAGCCAACGGTGAACAC	TTCCCCACTGATAAGCCAGC	CAACGCAGACCACCTTGACT	TGTTGGACCACAAGGGATCG

 

Amplicon 5'

Amplicon 3'

GAACAGCCAACGGTGAACACATAGCAGCCACACAAGCTCTATAGCTGATACAAGCAACGAAATACAAACAACGCAGTTTGTGTAAACAATCAAATTGTCGCAGCCATATCGAGTGTGCTTACACGTCCAGCGGAAAGTTTTCGAAACCAATCCAATCAATCAGCTAAGATGTCCGCTTCACCCACCGCCCGTCAAGCCATCACCCAGGCCCTGCCCATGATCACCAGGAAGGTTGTCATCTCGGATCCGATCCAGATGCCCGAGGTGTACTCCTCGACGCCCGGCGGAACCCTCTACTCCACCACTCCTGGAGGTAAGTTTCGAAAATTGGAAGCTGAGACTACATCGAGTATTATTAAGTTCCTTCCGGAAAGTGGAGTGATTTTCTTATCATTAGATATGATATCAAGATGAAATTAATCAACATATTTCGAAAACATAAACAAGTATACCGTTTCTTTCCTTTGCTCAAACAACAAACAAGCTTTTGTTTGAGTTGTGATACATTATTTTTGTAAAACTCACTCACCATTTTTTGGCTGATGAAACTGGGCCATCTCGCCGAGTCAGTCAGTTGGAAGATAATCCCGAGCACATGTTCCACAGTTCGTGACTCAGAGTAGCGCGGCAAAGAAGCGTTTACTCAGCTGGCTTATCAGTGGGGAA

CAACGCAGACCACCTTGACTAATAAAAATTTCCGCTTTCCTATAATTCCAGGCACCAAACTTATCTACGAGCGGGCTTTCATGAAGAATCTCCGTGGCTCCCCATTGAGCCAAACTCCGCCGTCCAACGTGCCCAGTTGCTTGCTGAGGGGAACTCCGCGTACTCCCTTCCGCAAGTGCGTGCCCGTCCCCACGGAACTGATCAAGCAGACCAAGTCGCTGAAGATTGAGGACCAGGAACAGTTCCAACTGGATCTGTAGGGGGTGTGGCGTGTACACCGCCATGCAGCAACTGCCAAATCCAACTAGTTCTTAGATGCACCCACTAAAACCGATCTTATAAGCTTAGTGTACCCATTAACTAGATGCTAAGTTCTTAGTTCTTCCACCTTGTTTGTTCTCTCGGTCATATTGACCCCGTTACCTTGTAAAATCGTAGATCTTAAGTAAAATGCAATAAAAAAAAGGCACACATTATTTAAAAGAGAACCACACCTTCCATTGGGATTCAGTAGCTGCCAATTTACAATTATAAATTTTAATTTATGTTTGAGAAATTCAGTGCCAAATTACTTTCTTTATCGGCAATTCAACCTTGGCAGGCCACGCGTTATGTGATAGAACACTTTCCACTTCACCTTGAGTCAGTTTCGGTTTAGTAGTATTTTCGGCAGTTTGTTGAACTGTTTGAACTGTTTACGAAGAAATTTAGCGAAAGGGGGCTTTTTGGGGGGCGGTACACATGTTAGACAGCTGGAAATACCGGCCGGCTTTTTAGTTAGCCAGTCAGTCAGTGGCCCCAGCAGCGCGTGTCCCACTTATTGCAAAGATATGCATACATATTTATAATGTATCCAAAAACCGGCAAACAAACGCCACAAAAAAATAGATCTTTAAGAAACTGCGCAGTTGGGAAACGACCTCGCCTTTGGCCCCCGCCCCGCCCTTTACCATTTTTCTTATATATACAAAAGTAGTGTGCTGAGCACATGTTCGTTCAGATAGACCGATCCCTTGTGGTCCAACA

 

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