CR00839

CRIMIC CR00839

Gene clos

CG42600

Information

Donor Location Cytology FBti Stock number
pM37-p12R:9599197..959921345F6FBti020070380680

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TATGAAAAGCGGGTCCTAAGTCGCGA TACGGCCACATAGTCGGTGTAGTCAA

Images

Construct Design Sequences

sgRNA sequence CATATGATGCCCGGTTTACCGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAGTTCCTGGCTGCTGATCC CCGGGTCTACTGAATCCTGTG ATGCGTGGAAGGTGATTCGG GCCGTCAATCCGCTGATCTA
 
Amplicon 5' Amplicon 3'
AAGTTCCTGGCTGCTGATCCGAAGGTACATTCCACGCAGATGTGTTCCCGAAAAGTATTGCACTGTCCGTATCCCCGTTTCCGGGGAAATCTCATAGATGGGTGATCCTTCACGGGCCTGGCTTACGGGCTCTGTTAGATTGTAGCTCACTGCTACGTAGCCACGCCCAGCGAAGGCCAGGCAATCCAGAGCCACTGCCTTAGGTGTTTCCAACTCCACGAGGAGCTGGTAGTCCTGCTCAGCTGGACGCCAGGTATAGACGGCAAAGTGACGCACTCCTGCGGCATTTTTATGCAAGGCCGTAGCGTACTTGACCGTGGGCAGTTGGAGGATGCAAATGTCAAGGGGAAAAGACACCGGCACGGAGGCCACTCGTTCTAGTCCATCGAAACTAAAGCTAGCTGGAAGAGGCATAAAGTGTTTTAGACAATAAGTTGCACAAACACTAATCACAAAAGATTCCTCTTCAGCTTTTTGTACTTTCATCTTTAGGATAGAGCCGTAGGTAATCCGTCGCCGCTGACTAAACCAAACAATTCTTTCTAACTCTTCGTTAGCCATGGCCGATGAGGAGCTGCAAACCTACCGTCGCTGCGTTGGCCTGCAGCTGGAGGAGCTGGAGCTGCTGAGCTCCATATACTGCGCGCCCGGAGAGCTGCATATGCTCGATGCCGGCGTAGTGGCTGATTTCAATGAGTTTCTCAAGGATGATAAGACAAAACCCGCTACTAGCCTTCTATCCCATTTGGAGTATGTGGTCAAATTGACTCTACCAGGCAAACAGTGCGTGGAAGTGCGAGTGGAACTGCCGCACCTATATCCGCTTTTGGAGCAGGCACGCGTCAGTGTACACACTACGCTACTGGGAAAATCCAAGGAGCAGCGCTTAAAGAATGATTTGGAGCAGTATCATGGTGAAAGGCGGGAGGAGGACGCCGAGCCGTACATCTTCCAGCTGCTGAGCTGGCTGCAGGACCGCATCGAGGATCTGTTAAAACGACCAGCTTCCGAGTTTGAAGTGCAGCAGGTAGCATCATCAGACCCGCAACAGCCACCAGCTACGGAACTTCAGAGAATTTGGATTTATTCGCATCACATCAAATCCAGTGCCAAGCGGCAGGAGCTGATTCGGCAGGCCCGACAGTTGGAGCTCACAGGATTCAGTAGACCCGG ATGCGTGGAAGGTGATTCGGCAAATGTACAGGAATTCTGGCGCACCATCAAGGCCCTGCGCTGGCAGAAGATCAGCGTGGTGCGGACGGAGCCTCGGCAGCGCAAGCGGGGATTCGAGGACTTCAGCGAACAGCTTTTTAATGCCGAAGAAGGCGTCATGAACATGGGCCAGTTCATTCGATTCCTCGAAGCCCACGGATTTGGCTACATGAAGTCGGAATTGTTTGGTTTAGCCTGAGCACTGGATTTCCCATCAAAATTTAACATGTTAATAAAGTTTGTTATATCATTTCAAACCTTGGTCGAAAAACTGAGGCACAGCAGTCGCCCGTTTGTGGAGCATCGATTCCTGATCTGAGTTCAGGAAGTGTGTGGGATAGGATTCCCGGAAGTTTACTTCAAAACTGATCACGCGGGCGATCACAGCCGCAAAGATCAACAAATTGCAACAGATTTGATACATATATTTATTTTGTGTAAACTATACAAAACGAAACATTCAAACTGTAACACGTGCACTGGTTGCAAAATAACTTTTACTACCGGCCATGATATTTTACGGTTCTTAAAGCTTTATTTAGTATGATTGTCATATGTTTGGTACCCTATATCATAGTGGGTGTATTAGAGCAGAGAGAAGTTCGTATTTTGGTACATTGGCTTTTAAATGTATTATTTGTTTCTCTTAAATTTCTTTTATATAAATCCGTCTGCCTCGATTTCTGATACAAATTCATTTTCAACTATCTTCGTTGACTTGGCCGATTGTTGTGCCGACGACATATCCTTGCTTATGTCTTTTTCTAAATTTCCTGCCTTTTGTGCTGCCTCTGAGGTTCGTTTTCCATTCTTCATAAAGGCACAGTAGCAGCAGTGGTCTGGTGATCGAGAAAACTTCTTCATTTGGGTGGCGTCTCGCCATCGGCGAACCCAAGAAATTTGGTAGTAGGCCCTTCTAAAGTTCTCATTGTTGAAGCCATAGATCAGCGGATTGACGGC
 
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