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CRIMIC CR00841

Gene PAN2

CG8232

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:8928370..8928372	44F8	FBti0199639	79311

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACATTGACGTTCTTCAGGGTTTGGG	ATTGAACTGCGGCTGAGGCGTTTGTA

Images

Construct Design Sequences

sgRNA sequence	GTGGGTCAAGGGGTCGCTTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGACTGAGCACTTCCACTT	CGACCCCTTGACCCACTTAG	TTTGGGGCCAACGCTCAC	AGCGATGGCAGGAACTTCAA

 

Amplicon 5'

Amplicon 3'

CGGACTGAGCACTTCCACTTTTTCCGGTGTAGATGCGCACATGACCATAGCCTCCGAGGTGACGCCGCGCATTTTGGCCGGCTTCAGGTTACACATGACCACTACCAGACGGTTCTGCATCTCTTCCAGGGGCACGAACTTCACCAGACCGGACACCACGGTGCGTGGAGCCGCCTCGCCGCAGTCGATCTTCTCCAGATACAAGCTGTCGGCGTCGGGATGGCGACCCACCTCCACAATCTTGCCCACGCGTAAATCCAGGCGACCGACGTCGACGGGAGCCTCCGGAGCGGCGGCGGGCTTCTCGGCGGCCGGTTTCTTCTCCTTGGACTTCTTTTCCTTGGGCTCCTTGGCCGGCTTAGGAGCCGGGGCAGCGGGTGCAGCGGTAGCAGGTCCAGCCTCTGCCGGCATAACTACTGGGGCCGCACTGGTGCAGAAACCCCGGGCTCCAGGCACCGGAATCTGTTTCTTGCCATTTCGCAGCTCCAGCTGCACCAGCTGGGCCAAAGCCGCCTCAACTTCCTTGGCCAGTGCAGCGTTCTCCTTGATCAGCTCCTGCTTCTGGCGCTCCACAAGTTGCTGCTGGATGCCGGAAATCTGGGGGGCAGTAGGTTATCGAGTGTTCGCAGGCGCTGGCAGGCGGAAATACTTGCCTCGGCTTCAATGGAGTTGATCAGCGCCTCGGCGCGCTCGTTGTTGCTTGCAATCTGCTGCAGATCGGCCATTGTTCTTTTGTTTCTCGCGAATTTTGAGCAATTCTGGCCGTAATTTTTACATGCGGATCCCACAGTCGTGGCAGCTCTACGCAGCATTGCAGTGCTTCTTCTTGCCACGCACAGTGGCGTCGCGCAGCGTTGGACAGGTAGTTCGTTGGCGGAGAAGCGAGAGTATTTTTACATCAGCCCTGCCAGGGTCACTCTAATCGGTGCAACGCAAAAACGGCTGGCAGTTTTGAGCGAAATTTGTTAAAAAATAAACAATAAAAAGTCGCGTGGGCATCGGCGTGCGGCAGATGGATTACGTGTATTGCGGCACCGACCCCATCGGGGCCTCCGAAGACATACTAAGTGGGTCAAGGGGTCG

TTTGGGGCCAACGCTCACCCACACGCACACACACACGCAAGCCAGCTAAAGCTTTCTCCTTTGTTTTCTGTTTACTTTTTGAAAAGGTGTTTATGACGCGGGATCTGCGCCGGGAAACGGGCACTTTTCGCCCAGCTTCAATGGATTCAACATCGGCACCACCGATCCGGAATATGTGGAGCTTGTGCCCGTGCTTGCCGACGGCGGGGAGCACTTCGGCGTTTCCAGCGTGGCGTTCGACGACTACGAAGAGCTGCTGTGGATGGGCAACCAGGGCGTGAGTTCCTCGTCCAGCCTGCCAGGCTTACTAATCCTGAATCTTCGCTCCTTCCCGCAGGGCCACGTCACATCGTACTACACAAACTCCATGCAGAAGTACACATCCTTTCAGGTCCACGCCACCGACATTGTGCGCGACATCTCCACCCTGGACTCCGGAGTGCTGGCGCTCACGCAGACCTCGCTGCGTCATCAGATCCGCAGGGGTCTGCCCAAGTTTACCTTCAAGTAAGCCACCGCGCTGGCACAAAGATTCCCCGAAAGTGAGGTTTCAATTTCTTGCAGATCGAACAACATGAAGGAGATGGTGAGCATGCTGCAGCTATCACCGCATCGTCTGGTCATGGCCGGACTGCAGGACGAGCTTATCGACTTCGACCTGCGCACGCTCAAGGAAACCCGCATAGAGCATGTGGGTGCCGGCGGATGCACGGTGCTACGCAAGAATTCACGTTATCTGTTTGCCGGCGACCAGCTGGGAACGGTAACGTTGCGCGACCTGAACAGCCTGAGTGTTCAGCACACAATCAAGACGCACACCAACATCCTTTCGGACTTCAGTGTCCAGGGAAATCTGCTAATCTCTTGCGGCTACAGCGGAAGGCAAAATAACCTGGCCATCGACCGCTTCCTAATGGTCTACGATCTGCGCATGCTGCGCCTGATCGCGCCCATTCAAGTGATGATTGATCCGCAGATGTTGAAGTTCCTGCCATCGCT

 

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