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CRIMIC CR00842

Gene ena

CG15112

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:19158083..19158083	56C1	FBti0199640	79312

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGGGTTTTCCACTCAGCTGCACAG	TGTTATTCTGGTTGGTCTGCGAGGTG

Images

Construct Design Sequences

sgRNA sequence	AATCCCATCGGTTTCAGGACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCTGCTGCGAACTACTGA	CCCATCGGTTTCAGGACAGG	ACCGATGGGATTAGAGGGGA	CGATGGCACCCACTTCTTCT

 

Amplicon 5'

Amplicon 3'

TTGCTGCTGCGAACTACTGAAATAATTTTAGTTGCATTAAAATTGTTTAAAAGAGTACAACACTTTTACAAAAGCAATTCTTTTGCTCACTCCGACTTCCTATAATTATTTTCGAATATGTAGTATCCAATTTATGAGATATAAATCGATTCGATTGTAAAAAACAGAAATTTAAAAAAACTGATTGACCGCTATTGTTTCGCAGTTTCTCATAGTGTGCAGAAAAAAAAAACTTCACTTTCGTTGCCACGAATTTCGATGCAAATCAAAGCGGACAGGCTCATAAGACTTAGCTCCAAACTGTCGGTGGTTATATAAGGCCCTGAAACGGCGCACAGCTGTGCCAGCCGCCAGAGTGCGACTCGGATTCGGATTGAGATTCGTCCGTCTGTCCAGCCGTCCGTACCGCCGATCTGCGCACTCCATTTAAGTGCAAAACTACTTGCGGCTTTTGGCCGGGCCAAGTCTGTAGCGAGAATACTGCCGACTCGACTCGTTTGGCGGCTGCTCTCCGTCATTATCTCTATGTGTCCCCCAGTAACACACAACTCGGAGCAACTTGGAGCAACTTGGAGCTGCTCAGGCAACTTGGAGCGGTGCAGGCTAGCAAAAACAAAAAAAGGGGCAGAGAAAAAAAGAAAAAAAAAAAGAACGGAGCTATAGGATAGAAAGAACACCACATAGCCAAGTTGAGCAAAAGGCAAGGCAACTCGTCATCGCCTTTGCATTTTTCCAATGAAGGCGTTCATTTGTCGCTGGCCATGTTTACTTCACTGGCTGACTTTAATTGTCAACTCAACTGAGTGTCCCGGGGGGAGAAAATGCTAAAGGTTGAATGCAGCATATACACGTTCATATTAGAACTTTCATTAGAATCATTTAATCGGTCCGTGCGACTCGAGACGATCATCTGGCTGGATGACAGGTGTGCGGGCATACAAAGAAATGCATTGCACTTCCTGTCCTGAAACCGATGGG

ACCGATGGGATTAGAGGGGAGTCGAGGGTATACATAGATCAAAGCCCTTGCTCACTTGAGGCGTGTGAAAAGTCTGGAAGATCACGGTGCAGTAAAGTGCAGTTGAATGAGTTCTTCAAGGGGCAAATTGTAGAGCCGATCTAATGATCTGAAAACGACTGCTCATTGCGACATTCTTTCTGGGAAGTCTTAATCGAGTCTAAGAAGTTTTTTTTATTATGACGGAAAATCGATTTCTATGAATATATGTAGAAGCTTTCCATAGAACAATATATACTTTTTACTTCCAATCCCACGAAAGCCATCAATAAATTTGTATCTCTTTTATTGAACTCGTTTAGTGCAAATAATAAAGTTGCCAAATTTGACTAATTTGTTAGCACAACTTCCGTTTCGTTTCCCACGATCTTTTAAGACTTTAAAAGAGTTAACATCATAAAATGGAATTTTTGTACATACATATATGTTTCTCGCACATTTTCATATCTTTTCTTCTCAAGGCAAGGGCTATTCCCTATAATAAATTTTCAGCTCGAACTTCTTGAAGTGTGTTAAGTCCTCATCATTTCCATACACATTCAAGGTTCCATTCCATACCATTTAAGTCAGCCAACTAACCATAATATTCACACTTATTTCTTGCAGTGAGCAGAGTATTATCGGGGCGCGGGCCTCTGTGATGGTGTACGATGACAACCAGAAGAAGTGGGTGCCATCG

 

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