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CRIMIC CR00845

Gene Sema1b

CG6446

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:17677086..17677086	54D5	FBti0199642	79314

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAATGAGCTCCAAGAGCAGACCTTC	GATCCGCTCTCCACAATCGGACTAAT

Images

Construct Design Sequences

sgRNA sequence	CTTGGGATATGATTGTCGGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAACAAAGTCCGGCTGGTTG	TGGGATATGATTGTCGGCGG	ATCCCAAGGTTGCCTGTGTG	TTGCTTTCCGGTCTTGTTGC

 

Amplicon 5'

Amplicon 3'

CAACAAAGTCCGGCTGGTTGAGTTGCTTGAGATCATATTGTTCGGTACGCAGGTTCTCCCTGTAGATGAGAGGATCTCCACCCGAAAAGTCCGCCACGGTGGCGCTGTACAAATGCCCGTCGGCGAAGGCGTACGTGCTATTGTGGGCGGGGCTGTAGGGGCACAGGCCCTGGGCCTCCACATCTCGGCTGACTTCGTAGCGCATGGCATGGGCGTGACCAGCAGAGCCGGCCTCCTCGGATGACACTTCCACCGGCGTGTAGTGACGACAGCGCGGCTTATACGAATTGGTGCCGCAGAGCAGCACCTCGCCATTGGGACGCAGTGCATAGACGCGAAGGTAGTTATGGCAATCCCACTCGTGCTTCCCCTTTAGGGCACACAGCTCCCGGTCCGCATCCGTGCTGTGCCACTCCAGACGCGCAATCTCCTTCAGGCCATTTAGGCTCACATTGTAGATGACGTCCCTGCAAATCGAGATAGAGAACAATTGAATAAACAAATGCGAGAATTAATACGCCGATGGCGTGTGCCTGCCCAGAATGTGAAATGGAATGGAAGCCAGCCAAGTACGGTGACCAAAAATAAACGACAAAAAACCCGTGAGTTGAGAGCTTCGTGACAGGAGCATACAAATGTTTGACTGAACAGTGCCGGTTAAAGTAATAGTTTTGAATAAGAACTATTGATAGATGTGTAAGCAAAGTACCTTACTTTCTTTGGCTCAAATACATTTGTTAATACAGCCTTAGCCTTAAGCAACGATTTTGCGACATTTCATTTTATTTAAGTAATCAAAAGCTTAAAAAGTAAACACTTGTTAGCAAGTTTTACAATTAATAAACAACGTGTTTTTTCATTAGCGACAGCCCAGGGTATTATTATTTCTTCACTTTGCATTAGAGCTTTTTCAAGAGCACACTACTATTATTTTGACCGCATCTGTTTTTACGCCCCGCCGACAATCATATCCCA

ATCCCAAGGTTGCCTGTGTGCAAATAATTAGAACTTGTACACAATTGTATAATTCAGCGTTTTAAGTGGCTAATTATTGTTGCTGCTAATTTGTTGCTATGTTCTTTATGGCACCAGCACGTCTGGGGCGCAAGAAAAATATGATAGATCGAAAGCTGGTTTGCATTTGTCGGTCCATTTCAGATCGCCGATAGCTGATTATTAGTCACCCAAATGGTAGTTTGCGTGGCTCTCATGTAAATGGTAATTATAAAATGTACATTATCGAAAAACCTATGCATTTAATATTTTTCAGTTAATAAATGTATATACCAGACTTCGTTGAAACGTGATAAAAAAAATAATGCCTTCTGACTTAACTTTGTTCATGAGCAATATAATTATATGGATGAAACTGAGCAAGTGTTATTTATTCCAGACCTTTGTATCTCGCCAATGCCAAATCACTTCGACTTCCCGGAAAGGGAATGAGAAATGCTAGATTTGCACTTGCCACTAATGAAAAACGAATACCAAGCCAAGCGGCCAATAAATCGGTAGAGTAAAGTAATGCCAAATGGCGAGCTAATTATTCCGAATCGGACGCTGTATAATTGTGTTTTTATAAATATTTAAACGTGTTCGTGCCAAGTTTATTTGCGCACAGGTAGCTTGCTCGGTAGCGGGAACTTTTTTATTGCTTGCCAGAAATCAAAGGGATCGTAAAAGAATGTTAAAGAAATTCACTCACTTGGCACCAACTAGAACAAACTCATCGTTGTGGTCCAAAATCTTGAAATAGTCCGTCGAGTTGCCTATAAAATGGGCTAGGACTTTGTCTTGTTCTGCAAAGAAAGAAAGAAGCAAAGTGAAATGTTATTGACTGCTGGGATTATATAACTTGTGCGTTTACTCGTGTTTTTTTTTTTTTTTTTTTTGAGATCTTGCACAATTTCATTTTAATTTCTGTTTAACTCGATTCAGAGGAAATGCTAGCGCTTGTTGCTGCTGGCATTGCGAAAATTCCGCCTGATAAGCAGCTTGGAAAATCGGAGCTTAGCTTGAACAGCCTCGCTTTTGTCATTGATAAACCAGCAGCAACAAGACCGGAAAGCAA

 

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